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Tai Ma, Wei Shi, Jun Cheng, Jian-Kang Zhang, Li-Fen Hu, Ying Ye, and Jia-Bin Li,*
Abstract: Chromobacterium violaceum, Gram-negative Bacillus, is a common inhabitant of soil and stagnant water found in tropical and subtropical regions of the world. It is a rare cause of severe, often fatal, human disease. In the report, 3 cases of patients infected with C. violaceum were described in Anhui Province, China. Routine and bacteriological investigations were carried out to establish the aetiological diagnosis. Moreover, the patients were treated with appropriate antimicrobial agents and auxiliary therapy. To our knowledge, a total of 42 cases have been reported previously from Chinese mainland in the recent 20 years, with a review of the literatures.[...] Read More.
Keywords: Chromobacterium violaceum, infection, China.
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Shaukat Abro, Xiaohong Tian*, Donghai You, Yuling Ba, Meng Li, and Faqi Wu
Abstract: A laboratory study was conducted to investigate efficacy of locally produced microbial inoculants with different concentrations on microbial respiration from soils with and without addition of wheat straw incubated at 20 and 25°C temperatures for 73 days. The results indicated that the soil microbial community structure and activities were obviously influenced by microbial inoculants. In general, whereas all inoculants levels were successfully established to different extents on the straw, none significantly improved decomposition in either soils or straw amended treatments. Additions of microbial inoculants expedite the decomposition and CO2 evolution process. CO2-C emission was regularly determined and results revealed microbial inoculants had little (10%) while straw addition significantly increased decomposition rates and cumulative CO2-C by 39% in straw amended than controls. Straw addition into soil had significant relationships (p < 0.05) with cumulative amounts of C; soil organic C, microbial biomass nitrogen. There was highly significant relationship between CO2-C emission and incubation period (R2=0.94) . In conclusion, microbial inoculants and straw incorporation will enhance CO2-C evolution, soil carbon and microbial biomass, thus improving soil quality.[...] Read More.
Keywords: Microbial inoculants, soil CO2-C evolution, decomposition microbial biomass, organic carbon.
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H. Zuridah,*, Sufiyan Hadi M, L. K. Teh and A. H. Zed Zakari
Abstract: Rotavirus and recently norovirus have been described as important and most common cause of acute gastroenteritis in children. The mode of transmission is fecal-oral. Diagnosis of both of these viruses can be made by rapid antigen detection of the viruses in stool specimens and strains can be further characterized by enzyme immunoassay or reverse transcriptase polymerase chain reaction. Stool specimens collected from various hospitals in Malaysia were examined for norovirus by both immunochromatography and reverse-transcription polymerase chain reaction techniques. Rotavirus antigen was screened by a commercially available latex agglutination test kit. Altogether, 168 stool samples were collected for both norovirus and rotavirus screening. Out of these, 77/168 (45.8%) were examined for rotavirus with 17/77 (22%) rotavirus antigen positive. Due to very small amount of fecal materials obtained in some cases, only 151/168 (89.8%) were sufficient for norovirus and 14/151 (9.3%) were positive for norovirus genogroup II (GII). This study highlights that rotavirus remains the main agent for acute gastroenteritis and identification for emerging norovirus among the children is becoming important for proper patient management.[...] Read More.
Keywords: Norovirus, rotavirus, immunochromatography, latex agglutination, RT-PCR, phylogenetic analysis.
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M. Monajjemi*, F. Naderi, F. Jadidi and F. Mollaamin
Abstract: The polarized continuum model (PCM) model has been used to optimize 2,66-trimethyl-1,3-cyclohexadiene-1-carboxaldehyde (safranal) in aqueous phase, methanol and carbon tetra chloride at B3LYP/6-31G** level of theory and the solvent effect has been studied. The principle of maximum hardness has been tested by calculating chemical hardness and chemical potential at B3LYP/6-31G** level of theory to predict the order of stability of the structure in solutions. The maximum IR intensity is related to the C4=C5 stretching vibrations. Also, the vibrational free energy, heat capacity, entropy, thermal energy and zero point vibrational energy obtained from the calculated frequencies and these are compared in different media.[...] Read More.
Keywords: Polarized continuum model (PCM), functional theory methods (DFT), solvent, IR, safranal.
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Farah Nadia Omar, Nor’Aini Abdul Rahman*, Halimatun Saadiah Hafid, Tabassum Mumtaz, Phang Lai Yee and Mohd Ali Hassan
Abstract: Polyhydroxybutyrate (PHB) was produced by Cupriavidus necator CCGUG 52238 using organic acids from fermented kitchen waste. HPLC and nuclear magnetic resonance (NMR) analyses revealed that the acid comprised mainly of lactic and acetic acids. In shake flask culture, the lactic acid concentration above 10 g/L inhibited both cell growth and polyhydroxybutyrate (PHB) production. The PHB production by the strain was achieved at the highest PHB content of 52.79% in batch fermentation using the kitchen-waste derived organic acids. The PHB yield and productivity were 0.38 g/g and 0.065 g/L/h, respectively. In fed-batch culture, about 4-fold increase in PHB productivity (0.242 g/L/h) was achieved by applying intermittent feeding strategy.[...] Read More.
Keywords: Cupriavidus necator CCGUG 52238, kitchen waste, organic acids, polyhydroxybutyrate (PHB).
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Full Length Research Paper
Abstract: Microbial degradation of Tapis crude oil contaminated soil by ACINETOBACTER BAUMANNII T30C was conducted to evaluate the efficiency of the selected potential hydrocarbon degrader in stimulating bioremediation of crude oil-contaminated soil with different treatment units in microcosms with 2.5 kg soil. Previously, A. BAUMANNII T30C was isolated from a Tapis crude oil-contaminated soil of oil refinery plant, Terengganu, Malaysia. The reduction of residual hydrocarbons in the soil was observed for a period of about 35 days. The study showed that amendment of nutrients was needed for stimulating the growth of A. BAUMANNII T30C and indigenous microorganism in assisting the degradation of residual hydrocarbons in the soil.[...] Read More.
Keywords: Bioremediation, bioaugmentation, aliphatic hydrocarbons, amendment of nutrients.
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R. M. Coopoosamy* and K. K. Naidoo
Abstract: Tetradenia riparia has been used by traditional healers for treatment against various ailments including wound healing and skin sores. The scientific validation of the use of T. riparia against selected strains of Gram positive and Gram negative bacteria as well as selected fungal strains has substantiated the use by traditional healers. The relatively high inhibitory concentration for both bacterial and fungal strains further indicated the high value of this plant species in the medicinal world and could aid in treatment of secondary infections, such as, mouth sores, pelvic or vaginal sores in individuals who are HIV/AIDS infected.[...] Read More.
Keywords: Tetradenia riparia, iboza, antimicrobial, traditional medicines.
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Tatjana Kundakovi *, Marina Milenkovi , Aleksandra Topi , Tatjana Stanojkovi , Zorica Jurani and Branislava Lakuši
Abstract: Cytotoxicity and antimicrobial activity of cyclohexane, dichlormethane and methanol extracts of Teucrium scordium subspec. scordioides was studied. Cyclohexane and dichlormethane extracts of T. scordium possessed high citotoxicity against MDA-MB- 361 cells (IC50=130.33±0.1 g/ml and IC50=189.89±3.99 g/ml, respectively). Dichlormethane extract was more effective against MDA-MB-453 cell line (IC50=130.33 ± 0.1 g/ml). The methanol extract of T. scordium possessed no cytotoxicity against breast cancer cell lines, MDA-MB-361 and MDA-MB-453. Herb extracts of T. scordium have shown weak antibacterial activity on Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli and Bacillus subtilis with no activity against Staphylococcus aureus, S. epidermidis, Micrococcus luteus, Enterococcus faecalis, and Candida albicans.[...] Read More.
Keywords: Teucrium scordium subsp. scordioides, cytotoxicity, antimicrobial activity.
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Isibor, J. O., Samuel, S. O.*, Nwaham, C. I., Amanre I. N., Igbinovia, O. and Akhile, A. O.
Abstract: Bacterial and other pathogens often infect the female genital tract causing disease in most women, sometimes with severe complications. A number of vaginal infections present with few or no symptoms and yet could still be transmissible to sexual partners of infected persons. Poor socio- economic status, lack of diagnostic facilities and shortage of effective treatment all contribute to the high incidence of sexually transmitted and reproductive tract infections. In this study we investigated mixed bacterial and Candida albicans infections in antenatal and gynecology attendees in Irrua Specialist Teaching Hospital Irrua, Edo State, Nigeria. Seventy-five high vaginal swab specimens were collected from female patients (18- 49 years) attending ante-natal and gynecology clinics of the hospital. Specimens were collected from symptomatic and asymptomatic patients and were analyzed using standard microbiological methods. Wet preparations were examined microscopically for presence of white blood cells (WBC) which suggested an infection, and yeast cells. The swabs were inoculated on MacConkey, Blood, and Chocolate agars, incubated at 37°C for 24 h, as well as Sabouraud Dextrose agar, incubated at room temperature for a few days. Of the seventy-five specimens analyzed, 56 (74.7%) isolates were from symptomatic patients while 25 (44.6%) were from asymptomatic patients. C. albicans was the most isolated pathogen with 35 (47.7%) isolates, followed by Staphylococcus aureus with 25 (29.8%), E. coli, 11 (13.1%), Klebsiella spp, 5 (6.0%) Enterococcus faecalis, 4(4.8%), Proteus spp 3 (3.6%) and Pseudomonas aeruginosa, 1(1.2%). This analysis revealed that candidiasis caused by C. albicans and infection with S. aureus is still the major health problems among females in this locality. The need for regular check- up at the clinics as well as personal hygiene is highly recommended in order to forestall avoidable infections.[...] Read More.
Keywords: Bacteria, Candida albicans, infection, antenatal, gynecology, attendees, Irrua, Nigeria.
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文章
Mohammad Kargar * Akram Najafi and Keivan Zandi and Zahra Hashemizadeh
Abstract: Rotaviruses are recognized as the most common causes of severe gastroenteritis and death among children worldwide. The aim of this study was to evaluate the disease burden of rotavirus gastroenteritis and the prevalence of different G genotypes of rotaviruses circulating in children aged[...] Read More.
Keywords: Human rotaviruses, diarrhea, genotypes, children, surveillance.
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