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Tatjana Kundakovi *, Marina Milenkovi , Aleksandra Topi , Tatjana Stanojkovi , Zorica Jurani and Branislava Lakuši
Abstract: Cytotoxicity and antimicrobial activity of cyclohexane, dichlormethane and methanol extracts of Teucrium scordium subspec. scordioides was studied. Cyclohexane and dichlormethane extracts of T. scordium possessed high citotoxicity against MDA-MB- 361 cells (IC50=130.33±0.1 g/ml and IC50=189.89±3.99 g/ml, respectively). Dichlormethane extract was more effective against MDA-MB-453 cell line (IC50=130.33 ± 0.1 g/ml). The methanol extract of T. scordium possessed no cytotoxicity against breast cancer cell lines, MDA-MB-361 and MDA-MB-453. Herb extracts of T. scordium have shown weak antibacterial activity on Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli and Bacillus subtilis with no activity against Staphylococcus aureus, S. epidermidis, Micrococcus luteus, Enterococcus faecalis, and Candida albicans.[...] Read More.
Keywords: Teucrium scordium subsp. scordioides, cytotoxicity, antimicrobial activity.
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Isibor, J. O., Samuel, S. O.*, Nwaham, C. I., Amanre I. N., Igbinovia, O. and Akhile, A. O.
Abstract: Bacterial and other pathogens often infect the female genital tract causing disease in most women, sometimes with severe complications. A number of vaginal infections present with few or no symptoms and yet could still be transmissible to sexual partners of infected persons. Poor socio- economic status, lack of diagnostic facilities and shortage of effective treatment all contribute to the high incidence of sexually transmitted and reproductive tract infections. In this study we investigated mixed bacterial and Candida albicans infections in antenatal and gynecology attendees in Irrua Specialist Teaching Hospital Irrua, Edo State, Nigeria. Seventy-five high vaginal swab specimens were collected from female patients (18- 49 years) attending ante-natal and gynecology clinics of the hospital. Specimens were collected from symptomatic and asymptomatic patients and were analyzed using standard microbiological methods. Wet preparations were examined microscopically for presence of white blood cells (WBC) which suggested an infection, and yeast cells. The swabs were inoculated on MacConkey, Blood, and Chocolate agars, incubated at 37°C for 24 h, as well as Sabouraud Dextrose agar, incubated at room temperature for a few days. Of the seventy-five specimens analyzed, 56 (74.7%) isolates were from symptomatic patients while 25 (44.6%) were from asymptomatic patients. C. albicans was the most isolated pathogen with 35 (47.7%) isolates, followed by Staphylococcus aureus with 25 (29.8%), E. coli, 11 (13.1%), Klebsiella spp, 5 (6.0%) Enterococcus faecalis, 4(4.8%), Proteus spp 3 (3.6%) and Pseudomonas aeruginosa, 1(1.2%). This analysis revealed that candidiasis caused by C. albicans and infection with S. aureus is still the major health problems among females in this locality. The need for regular check- up at the clinics as well as personal hygiene is highly recommended in order to forestall avoidable infections.[...] Read More.
Keywords: Bacteria, Candida albicans, infection, antenatal, gynecology, attendees, Irrua, Nigeria.
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Mohammad Kargar * Akram Najafi and Keivan Zandi and Zahra Hashemizadeh
Abstract: Rotaviruses are recognized as the most common causes of severe gastroenteritis and death among children worldwide. The aim of this study was to evaluate the disease burden of rotavirus gastroenteritis and the prevalence of different G genotypes of rotaviruses circulating in children aged[...] Read More.
Keywords: Human rotaviruses, diarrhea, genotypes, children, surveillance.
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Mohammad Taghi Akhi, * Tahere Pirzade, Behruz Naghili, and Mortaza Gojazade
Abstract: The aim of this study was to determine antibiotic susceptibility of Clostridium difficile isolated from different sources. Two hundred stool samples of staff, patients (at admission to the wards and the same patients after seven days of hospitalization), and 135 samples of hospital environment were collected. Three standard methods including direct plating onto cycloserine-cefoxitin fructose agar, alcohol shock and enrichment culture with 0.1% sodium taurocholate were used to isolate C. difficile. All C. difficile isolates identified by biochemical tests and were tested by disk diffusion agar using 15 antibiotic disks. MIC of isolates was determined for vancomycin and metronidazol by Etest. Seventy C. difficile were isolated from different sources. No resistant isolates to vancomicin and metronidazol were detected by disk diffusion or Etest. The rate of recovery by 0.1% sodium taurocholate enrichment method and alcohol shock was significantly higher than those by CCFA (pv 0.02, pv 0.04).[...] Read More.
Keywords: Clostridium difficile, antibiotic resistance, vancomycin, metronidazol.
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Jethwani Urmi N.*, Mulla Summaiya A., Shah Latika N. and Panwala Tanvi R.
Abstract: Clindamycin is commonly used in treatment of erythromycin resistant Staphylococcus aureus causing skin and soft tissue infections. In vitro routine tests for clindamycin susceptibility may fail to detect inducible clindamycin resistance due to ‘erm’ genes resulting in treatment failure thus necessitating the need to detect such resistance by a rapid method. In the era of automation, vitek-2 system provides a panel for detection of inducible clindamycin resistance with conjunction of other antimicrobial susceptibility testing. The present study evaluated the performance of a vitek-2 card for detection of inducible clindamycin resistance in a tertiary care hospital. Non-duplicate clinical isolates of hundred S. aureus were obtained from various clinical samples. Antimicrobial susceptibility testing was carried out, including detection of clindamycin resistance and methicillin resistance pattern by vitek-2 identification and antimicrobial susceptibility testing (ID/AST) system by using AST-GP67 card. The results were compared to those of D-test as per CLSI guidelines on erythromycin resistant isolates. EPI INFO software, Licensed by CDC Atlanta was used for analysis of data. The sensitivity and specificity for the vitek-2 card was 95.4 and 100%, respectively in comparism to disk approximation test (D-test),. The performance of vitek-2 card was 100% specific and rapid for detection of inducible clindamycin resistance with other antimicrobial susceptibility results.[...] Read More.
Keywords: Automated system, constitutive macrolide-lincosamide-streptogramin B (MLS B) phenotype, inducible MLSB phenotype, methicillin resistance Staphylococcus aureus (MRSA), msrA gene (MS phenotype).
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ADON Marie Paulette*, OUATTARA Allassane and GOURENE Germain
Abstract: Phytoplankton species composition and seasonal changes were investigated in the shallow reservoir of Adzopé. Taxonomic composition, diversity and abundance of phytoplankton were studied at 4 stations from May 2008 to February 2009, in relation to physical and chemical factors (temperature, conductivity, dissolved oxygen, transparency, pH, nutrients) and climatic factor (rainfall). The phytoplankton comprised 144 taxa, mainly Chlorophyta (29.45% of taxa), Euglenophyta (29.45%) Bacillariophyta (23.97%) and Cyanobacteria (10.27%). Phytoplankton density values were greatest during the transition season (short rainy season), lower during the dry seasons, and extremely lower during the long rainy season (mean value 356 105 cells/l, 44 105 cells/l and 35 105 cells/l respectively). Abundance was dominated by Anabaena constricta Szafer (Geitler) and Microcystis aeruginosa (Kütz.) Kütz. The diversity index varied between 2.1 bits/cells in long rainy season and 4.6 bits/cells in short dry season. The redundancy analysis (RDA) demonstrated a separation between the long rainy season and the other seasons due to the influence of the flood pulse.[...] Read More.
Keywords: Phytoplankton, shallow reservoir, species dominance, West Africa.
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Essack, S. Y. and Connolly, C.
Abstract: Nationally-devised standard treatment guidelines (STGs) for nosocomial infections were evaluated in the context of antibiotic resistance within the public health care system in Kwazulu-Natal. A multi-centre surveillance study instituted in 3 hospitals at 3 progressive levels of health care (district, regional and tertiary) collected consecutive, non-repetitive isolates commonly implicated in nosocomial infections as cited by the STGs, viz., Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa and Acinetobacter spp. Isolates were subjected to susceptibility testing against antibiotics recommended in the treatment guidelines as empirical treatment for nosocomial infections using the Kirby Bauer disc diffusion method advocated by the CLSI. Percentage susceptibility across (1) bacterial species, (2) antibiotics and (3) hospital levels was compared. Susceptibility to antibiotics recommended in the treatment guidelines and hence potentially successful empiric therapy ranged from 5 to 95% with multi-resistance evident in all isolates. Statistically significant differences in overall susceptibility were observed (1) across bacterial species, (2) within 2 of the 3 bacterial species for different antibiotics and; (3) across hospital levels for 2 antibiotics with p values[...] Read More.
Keywords: Treatment guidelines, nosocomial infections, antibiotic resistance.
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Full Length Research Paper
Abstract: Present investigation was the first attempt which deals with the IN VIVO and IN VITRO comparative study of protein level in ACACIA NILOTICA L., a nitrogen fixing tree. Protein was investigated in callus, seed, leaf and stem by means of SDS-PAGE. For obtaining the IN VITRO explant, the seeds were cultured on ½ MS medium under the IN VITRO condition. The cotyledonary nodal explants were taken from the IN VITRO seedlings and cultured in the MS medium supplemented in combination of 2, 4-D (0.4 mg/l) with BAP (0.25 mg/l). Though some differences were observed in the protein contents of IN VIVO and IN VITRO samples, the data proved that protein content in callus was higher than the seed following leaf and stem. In this study it was found that A. NILOTICA contained several protein bands of molecular weight 54.3, 44.1, 42.7, 40.1, 35.6, 31.2, 28.6, 24.7 and 19.5 kDa. These results indicate that the intensity of protein bands was high in IN VITRO sample compared to IN VIVO samples.[...] Read More.
Keywords: SDS-PAGE, in vitro, in vivo
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Full Length Research Paper
Abstract: Diphtheria is an acute bacterial disease caused by oxygenic strains of Corynebacteria. Fatality rate of this disease, between 5 and 10%. In children under 5 years and adults, the fatality rate may be as much as 20%. Outbreaks, although very rare, still occur worldwide, even in developed nations. Diphtheria toxin (DT) is the major virulence factor for these organisms. The aim of this study was cloning and expression diphtheria toxin gene to produce recombinant protein and application in next investigations. The bacterial DNA was extracted and amplification of diphtheria toxin gene was carried out with specific primers. This gene was cloned in pTZ57R/T vector and sub cloned into pETDuet-1 expression vector then recombinant plasmid was transformed into BL21 of Escherichia coli strain and induced by IPTG. Diphtheria toxin gene was amplified successfully and cloned in pTZ57R. Recombinant plasmid was digested by restriction enzymes and released fragment (diphtheria toxin gene) sub cloned in pETDuet-1 expression vector and expressed protein was analysed in serological assay. In this study, the diphtheria toxin gene was cloned in pETDuet-1 expression vector and confirmed by sequencing and restriction analysis then recombinant plasmid was transformed in BL21 expression cell.[...] Read More.
Keywords: Diphteria, Corynebacterium diphtheriae toxin gene, recombinant protein, pETDuet-1.
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Xiao-Ling Ma, Fei-Hu Chen*, Xin Zhou, Wen-Jiao Chang and Yuan-Yuan Dai
Abstract: The aim of this study is to investigate the genetic backgrounds of methicillin susceptible Staphylococcus aureus (MSSA), methicillin-resistant Staphylococcus aureus (MRSA) and heterogeneous vancomycin-intermediate Staphylococcus aureus (hVISA) isolates isolated from clinical specimens of the patients with verified infections in a Chinese teaching hospital. Macro E test (MET) was used to detect hVISA and multilocus sequence typing (MLST) was used to determine the STs of the selected isolates. The genotypes of SCCmec were determined by multiplex polymerase chain reaction (PCR) in MRSA isolates. Panton-valentine leucocidin (PVL) genes were also detected by PCR. Among 273 S. aureus isolates, hospital-acquired methicillin-resistant Staphylococcus aureus (HA-MRSA), community-associated methicillin-resistant S. aureus (CA-MRSA), hospital-acquired methicillin susceptible Staphylococcus aureus (HA-MSSA) and community-associated methicillin susceptible Staphylococcus aureus (CA-MSSA) isolates accounted for 55.6, 1.5, 36.3, and 6.6%, respectively. Nine isolates were confirmed as hVISA by MET. Among 60 HA-MRSA isolates, ST239-MRSA-III was the most prevalent clone accounting for 51.7%, followed by ST5-MRSA-II clone. Fifty percentage and 22.2% of CA-MSSA isolates were found to be ST121 and ST88. ST239-MRSA-III was the predominant clone in hVISA isolates. However, no predominant ST type was found in HA-MSSA isolates. Of 9 PVL-positive strains, ST88 was the most prevalent ST (50.0%; 4/8), followed by ST121 (33.3%; 3/9), ST5 (4.5%; 1/22) and ST239 (2.6%; 1/39). In conclusion, ST239- III was the major pandemic clone in hVISA and HA-MRSA and spread in China. ST5- emerging rapidly in China had remained stable viability.[...] Read More.
Keywords: Staphylococcus aureus, heterogeneous vancomycin-intermediate Staphylococcus aureus, molecular characteristic.
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