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Rabbani Syed,*, Giri Prasad, Farha Deeba, Rani D. , Kaiser Jamil, and Ali A. Alshatwi
Abstract: Nosocomial infections are one of the occupational biohazards that affect the health of individuals with or without predisposing factors. Staphylococcus aureus is associated with significantly higher mortality and is associated with community-acquired serious nosocomial infections because strains generally show multiple drug resistance, which limits treatment possibilities. A total of 1800 patients in the state of Andhra Pradesh were screened for the presence of Staphylococcus species and were tested for antibiotic resistance. The results indicated that among ten antibiotics used in the present study, Amikacin and Azithromycin should be the drug of choice to treat S. aureus infection. It was observed that the resistance of most of the antibiotics tested showed increased resistance with increasing age. These results suggest that clinicians should consider age as an important factor while prescribing these antibiotics.[...] Read More.
Keywords: Nosocomial, antibiotic resistance, Staphylococcus aureus.
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J. Lin* and C. Harichund
Abstract: Thirteen metal-tolerant bacteria capable of producing metal- removing bioflocculants were isolated from an industrial effluent sample. Pseudomonas sp. was found to be the pre-dominant species among the isolates (8 out of 13), followed by Herbaspirillium spp. (4) and one Paenibacillus sp. The flocculating activity of bioflocculants produced by these microorganisms was assayed using the kaolin clay. The heavy-metal-removal efficiency was determined using atomic absorption spectrometer before and after mixing the bioflocculant with the heavy metal solutions. Bioflocculants exhibit different flocculating abilities of removing kaolin clay in the presence of different heavy metals. Bioflocculants produced by Pseudomonas sp. CH9 possessed the highest flocculating activity (1.8) compared to the remaining bioflocculants. The flocculating activities of CH11 and CH13 increased to 0.95 and 0.87 in the presence of Pb2+ and to 0.89 and 0.98 in the presence of Hg2+ respectively from 0.015 in the presence of Ca2+ in the standard kaolin clay assay. Up to 90% of Pb2+ was removed by Pseudomonas sp. CH8 bioflocculants. Seventy-eight percent of Hg2+ and 66% of Cd2+ was removed by Pseudomonas sp. CH6 and Herbaspirillium sp. CH13 bioflocculants respectively. Most of the bioflocculants demonstrated a higher percentage of heavy-metal removal at low concentrations. This study demonstrates that microbial bioflocculants have potential to be used as an alternative bioremedial tool for industrial effluents and wastewater treatments which are co-contaminated with heavy metals.[...] Read More.
Keywords: Bioflocculant; heavy metals, Pseudomonas sp., Herbaspirillium sp., Paenibacillus sp., Industrial effluent.
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S. J. Shaibu*, H. M. Kazeem, U. S. Abdullahi and M. Y. Fatihu
Abstract: Dermatophilus congolensis is the causative agent of dermatophilosis an economically important disease of livestock, and also an agent of zoonotic importance. The disease has been reported worldwide, with a wide host range which includes domestic, wild and aquatic animals. This study was therefore undertaken to characterize isolates of the organism from cattle, sheep and goats in Nigeria. All the isolates, except two sheep isolates fermented glucose and sucrose. The whole cell protein profiles of the isolates were similar at about 62 and 20 KDa, but different at other levels. The polymerase chain reaction (PCR) of the partial 16SrRNA gene amplified all the isolates, but not other organisms included. The multiple sequence alignment of the PCR amplicons sequences showed an identity of between 98.5 and 100% across all the isolates. There was also a sequence similarity of between 99.2 and 99.76% between the isolates and the partial sequence of the type strain of D. congolensis DSM 44180T in the Genbank. Based on these techniques it may be concluded that all the isolates are the same with minor differences which were not enough to speciate them.[...] Read More.
Keywords: Dermatophilus congolensis, phenotypic, genotypic, sds-page, polymerase chain reaction, sequencing, multiple sequence analysis.
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Nesibu Awol, Gelagay Ayelet, Shiferaw Jenberie*, Esayas Gelaye, Tesfaye Sisay and Haileleul Nigussie
Abstract: This study was carried out with the aim of identifying bacterial species involved in lung lesions of camels slaughtered between October 2009 and April 2010 at Addis Ababa abattoir enterprise, Ethiopia. All camels were originated from Borana and Kereyu pastoral areas. A total of 387 lungs were inspected during the study period, of which 300 (77.5%) possessed gross pulmonary lesions. Of which 72 lungs with lesions were processed for bacteriology and bacterial growth was observed from 50 of the pneumonic lung samples. A total of 54 bacterial species were isolated and identified. These included coagulase negative staphylococci (21.1%), Streptococcus species (19.3%), Escherichia coli (17.5%), Francisella tularensis (5.3%), Flavobacterium species (5.3%), Rhodococcus equi (5.3%), Bordetella bronchoseptica (3.5%), Aeromonas hydrophila (3.5%), Neisseria species (3.5%), Streptococcus agalactia (1.8%), Staphylococcus aureus (1.8%), Pasteurella trehalosi (1.8%), Pasteurella anatipestifer (1.8%), Pseudomonas aeruginosa (1.8%), Micrococcus species (1.8%) and Mycobacterium species (5.3%). These pathogens could induce respiratory diseases under stressful conditions or predispose camels to other opportunistic infections.[...] Read More.
Keywords: Bacteria, camel, pulmonary lesion, Ethiopia.
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Eligius F. Lyamuya, Sabrina John Moyo *, Ewaldo V. Komba and Martin Haule
Abstract: Urinary tract infections (UTIs) occur frequently among women with diabetes. The present study aimed at determining prevalence and risk factors of bacteriuria in diabetic women and antimicrobial resistance pattern of the isolates at Muhimbili National Hospital (MNH), Dar es Salaam. Three hundred diabetic women attending clinic at MNH from June to November 2010 were included in the study. Demographic and clinical information were collected using a structured questionnaire. Urine specimens were collected for urinalysis, microscopy, culture and antimicrobial susceptibility testing. Significant, asymptomatic and symptomatic bacteriuria was found in 13.7% (41/300), 13.4% (31/231), and 14.5% (10/69) diabetic women, respectively. The isolated pathogens were Escherichia coli (39.0%), Klebsiella pneumoniae (22.0%), coagulase negative Staphylococci (14.65) and Proteus spp. (12.2%). Both Gram positive and negative bacteria showed high rate of resistance towards co-trimoxazole (55.6% and 50.0%, respectively). Gram negative bacteria showed high rate of resistance to ampicillin (62.55%), penicillin (53.1%) and moderate resistance to cefotaxime (18.8%). Advanced age and glycosuria were significantly associated with bacteriuria (P < 0.05). E. coli was the commonest aetiological agent for both symptomatic and asymptomatic bacteriuria among diabetic women, especially those with advanced age and glycosuria. Most uropathogens were resistant to co-trimoxazole, ampicillin and ciprofloxacin.[...] Read More.
Keywords: Diabetes mellitus, bacteriuria, antimicrobial resistance.
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Asli Akpinar, Oktay Yerlikaya* and Sevda Kiliç
Abstract: The aim of this study was to determine the inhibitive effect of 25 Lactobacillus delbruecki ssp. bulgaricus and 16 Streptococcus thermophilus strains isolated from 30 different homemade yoghurts on several pathogen and contaminant bacteria. The antibiotic resistance of these bacteria was also determined. All of Lactobacillus bulgaricus strains exhibited antimicrobial activity against Escherichia coli, whereas all of S. thermophilus strains exhibited the same activity against Klebsiella pneumoniae. None of L. bulgaricus strains were resistant to the polymixin-B, only the OL4 strain has shown resistance to bacitracin. While some strains of S. thermophilus like C6 and SL4 exhibited resistance to novobiocin, SY72, M3, C1M, and F1M were shown to optochin. ET6 and SY73 strains were found to be resistant in both novobiocin and optochin.[...] Read More.
Keywords: Antibiotic resistance, antimicrobial activity, homemade yoghurt, lactic acid bacteria, Lactobacillus delbrueckii ssp. bulgaricus, Streptococcus thermophilus.
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Masniari Poeloengan
Abstract: Red ginger (Zingiber officinale Roscoe) a well known herb that was widely used as a remedy for various ailments in traditional medicine, belonged to the Zingiberaceae family. The red ginger had antibacterial properties. In the face of increasing bacterial resistance to various antibiotics and continuous efforts to look for new and safer antibacterial substances, the objective of this study was to assess the red ginger’s antibacterial potentials for treating-mastitis. Mastitis was a common milking cows’ disease that caused a tremendous economic loss to dairy farms. Several bacteria that is, Staphylococcus aureus, Staphylococcus epidermidis, and Streptococcus agalactiae were responsible for this loss. To achieve this objective 3 x 4 factorial experiment was implemented. Three bacterial isolates that is, S. aureus, S. epidermidis, and S. agalactiae were tested with the following each concentration of the red ginger extracts that is, 50, 25, 12.50, and 6.25%. The findings were as followed: (1) S. epidermidis was most affected by the red ginger extract, followed by S. aureus and S. agalactiae, (2) the higher the concentration of the red ginger extracts, the higher the bacterial growth inhibition effect, and (3) the growth inhibition effects of the red ginger extracts on S. aureus, S. epidermidis, and S. agalactiae isolates were highly significantly different at < 0.0001. Therefore, the red ginger the traditional remedy was effective in controlling the three mastitis causing bacteria’s growth.[...] Read More.
Keywords: Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus agalactiae, red ginger, traditional medicine, mastitis.
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文章
C. Alagesaboopathi
Abstract: The present study deals with the antimicrobial activity of the aqueous, acetone and petroleum ether extracts of the leaves, stem and root of Andrographis ovata Clarke, Aristolochia indica L., Eclipta prostrata L. and Gloriosa superba L., using agar diffusion method against human pathogens, such as Escherichia coli, Proteus vulgaris, Psudeomonas aeruginosa and Klebsiella pneumoniae. In the present investigation, all the extracts were found to be effective against four human bacterial species, E. coli, P. vulgaris, P. aeruginosa and K. pneumoniae, sensitive to all the plant extracts. The study suggests that the extract of the plant parts possesses potential broad spectrum antimicrobial activity. The antimicrobial activity of acetone extracts was found to be higher than that of distilled water extracts. However, the root extract showed more inhibitory effect than the stem and leaf extracts.[...] Read More.
Keywords: Medicinal plants, antimicrobial activity, plant extracts, growth inhibition, disc diffusion method.
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Ozlem Aycan Kaya, Hakan Oguzturk, Muhammet Gokhan Turtay, U. Nilgun Daldal
Abstract: Antigen screening were conducted to stool samples from 60 patients admitted to our emergency department with diarrhea complaint between June 2009 and October 2009 by the methods of direct microscopic examination, trichrome staining, ELISA (Enzyme-linked immunosorbent assay), respectively. As a result of examination of total 60 samples with direct microscopic, trichrome staining and ELISA method, it was detected positive in 7(11.3%), 6(9.7%) and 8(12.9%) samples, respectively. The presence of Entamoeba histolytica has been accepted exactly in the samples in which ELISA test results were positive and necessary treatment of patients has been started immediately. Due to precise pathogen protozoan discrimination has not been performed with the direct microscopic examination, it was emphasized that unnecessary drug therapy would be prevented as a result of detection of presence of E. histolytica specific antigen by ELISA in the samples sent to the laboratory with the diagnosis of amoebiasis by concerned physician.[...] Read More.
Keywords: Entamoeba histolytica, diarrhea, diagnosis.
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Ihtisham Bukhari*, Mukhtiar Hassan, Fida M. Abbasi, Ghulam Mujtaba, Nasir Mahmood, Noshin, Anees Fatima, Muhammad Afzal, Mujaddad Ur Rehman, Farzana Perveen and M. Tariq Khan
Abstract: The importance of medicinal plants in traditional healthcare practices provides opportunity to new areas of research. However, information on the uses of plants for medicine is lacking from many interior areas of District Mansehra. Keeping this in view, the present study was initiated. This study attempts to shade lights on the comparative pharmacological efficacy of Berberis lycium (medicinal plant) and drug penicillin G. The pharmacological activities of both the B. lycium and penicillin G were determined by disc diffusion method with incubation period of 24 to 48 h at 37°C. It was observed that, B. lycium showed excellent activity against the tested microorganism, Escherichia coli and Proteus (80 to 100%) and good activities against the tested microorganisms pseudomonas and staphylococcus (60 to 70%). While penicillin G showed excellent activity against all tested microorganisms. It is recommended that, B. lycium can be used for the treatment of diseases caused by tested microorganisms because of its excellent and good activity. It is also observed that, B. lycium do not have any adverse effect on those people who have this in long practice.[...] Read More.
Keywords: Berberis lycium, penicillin G, disc diffusion, pharmacological activity.
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