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Mei Li, Hao-Jie Dai, Zhen-Chang Wang*, Zhen-Gang Wang, Li Cui, Qing-Gang Xu and Jing Li
Abstract: To assess diagnostic effectiveness of Sjögren’s syndrome (SS), salivary gland scintigraphy (SGS), magnetic resonance (MR) and diffusion-weighted imaging (DWI) were performed in 54 patients (primary SS, secondary SS and connective tissue disease (CTD) but without SS). Results showed that SGS and diffusion-weighted magnetic resonance imaging (MRI) provided high-resolution images of the parotid glands that enabled apparent diffusion coefficient (ADC) measurements. After overall analysis, all of the oral activity indices, the maximum accumulation (MA) and uptake rate (UR) of the parotid gland clearly decreased as clinical severity progressed, and statistically significant differences were observed. Our technique may prove helpful in diagnosing the progression of SS beyond its early stages.[...] Read More.
Keywords: Diffusion-weighted imaging, salivary gland scintigraphy, Sjögren’s syndrome, receiver operating characteristic curve.
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Agbaje M.*, Davies R., Oyekunle M. A., Ojo O. E., Fasina F. O. and Akinduti P. A.
Abstract: Reports of heavy mortality in three commercial layer poultry farms and two meat-type turkey farms in Ogun State were received separately between March and December, 2008 at the Veterinary Teaching Hospital (VTH), University of Agriculture, Abeokuta, Nigeria. We investigated the cause of mortality, isolated and serotyped the aetiological agent, and carried out antimicrobial susceptibility testing. All five isolates serotyped were Salmonella Gallinarum and showed identical patterns of resistance and susceptibility to all antimicrobials used. The occurrence of ciprofloxacin resistance in all five isolates is of importance since fluoroquinolone resistance has implications for both veterinary and human therapy and the abuse of such medications in poultry could result in the emergence of resistant zoonotic organisms.[...] Read More.
Keywords: Occurrence, transmission pattern, Salmonella gallinarum poultry farms.
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Rihua Xu, Shimin Ma, Yang Wang, Lisha Liu and Pinglan Li*
Abstract: The exopolysaccharides (EPS) producing ability was evaluated by phenol -sulphuric acid method and the Lactobacillus HCT was the highest yield EPS-producing strain, which was isolated from feces of Bama centenarian in Guangxi of China. It was identified as Lactobacillus paracasei HCT with carbohydrate assimilation profiling and 16S rRNA gene sequencing. L. paracasei HCT was found to be a novel EPS producing strain. Preliminary one factor tests were employed to obtain the favorable conditions for EPS yield and microorganism growth in a chemically defined medium and found that carbon nitrogen ratio (C/N ratio), cultivation time and temperature had the most significant influences. Box–Behnken experimental design and response surface methodology (RSM) were adopted to further study the interactive effects of these three variables on EPS yield and cells growth. The optimal culture conditions for EPS production were: C/N ratio 9.090, cultivation time 60.67 h and temperature 29.2°C. In these conditions, the maximum EPS yield was 39.0736 mg/ml which was 4 times more than the original yield. But the optimal conditions for cell growth were: C/N ratio 8.643, 58.75 h and 31.9°C, logarithm value of viable cell count reached 7.921. These predicted values were also verified by validation experiments.[...] Read More.
Keywords: Lactobacillus paracasei, exopolysaccharides, chemically defined medium, Box-Behnken design.
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Arvind Kumar, Poonam Bhargava and Lal Chand Rai*
Abstract: Six phosphate solubilizing bacteria (PSB) were isolated from paddy fields of Eastern Uttar Pradesh, India harboring low available phosphorus. Taxonomic delineation employing morphological, biochemical, 16S rRNA gene sequences and phylogenetic affiliations suggests that they are members of Enterobacter and Exiguobacterium genera. Of the six isolates, Enterobacter sp. LCR1 and LCR2 exhibited high level (568 - 642 g/ml) of phosphate solubilization in NBRIP liquid medium. Exiguobacterium sp. LCR4 and LCR5 showed increased phosphate solubilization efficiency under alkaline pH while Enterobacter sp. LCR3 remained unaffected. At high salt and temperature, Enterobacter sp. LCR1 and LCR2 produced 1.6 fold soluble phosphorus in comparison with earlier studies. Thus, these isolates may be useful for the development of potential bio-inoculants for soils having alkaline pH, high salt, temperature and insoluble phosphorus.[...] Read More.
Keywords: Phosphate solubilizing bacteria, phylogenetic analysis, Enterobacter spp., Exiguobacterium spp., 16S rRNA gene sequencing.
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Desmond M. Ncango, Chantel W. Swart, Carolina H. Pohl, Pieter W. J. Van Wyk and Johan L. F. Kock*
Abstract: We investigated the effects of anti-inflammatory and anti-mitochondrial compounds on spore dispersal in the pathogens, Aspergillus fumigatus and Rhizopus oryzae. When acetylsalicylic acid (ASA) and other non steroidal anti- inflammatory drugs (NSAIDs) were added to bio-assays of A. fumigatus and R. oryzae, spore-releasing structures were targeted first at lower concentrations. Similar results were obtained when oxygen was limited. These spore-releasing structures contained increased levels of mitochondrion activity compared to hyphae. We concluded that increased mitochondrion activity is necessary for dispersal of A. fumigatus and R. oryzae.[...] Read More.
Keywords: Aspergillus fumigatus, bio-assay, mitochondrion activity, non steroidal anti-inflammatory drugs, Rhizopus oryzae, spore-releasing structure.
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Ava Behrooozi, Mohammad Rahbar * and Jalil Vand Yousefi
Abstract: Urinary tract infections (UTIs) are one of the most common infectious diseases diagnosed in communities and hospitalized patients. The aim of this study was to determine frequency of occurrence and antimicrobial susceptibility patterns of uropathogens in Milad hospital of Tehran, Iran. In a prospective study from March to June 2009, a total of 11308 urine sample from patients admitted in Milad hospital of Tehran were analyzed. All specimens were inoculated on routine culture media. Bacterial isolates were identified by conventional bacteriological methods. Susceptibility testing was performed by standard methods as recommended by clinical laboratory standard institute. 11308 urine samples were cultured and 1020 pathogen were isolated. Escherchia coli with 620 (60.78%) isolates was the most common causative agent of UTI followed by Klebsiella pneumoniae with 115 (11.27%) isolates. Among gram positive Cocci Enterococcus spp with 110 (10.78%) isolates and Staphylococcus aureus with 81 (7.94%) isolates were predominant organisms. Of 1020 patients, 227 (22.25%) were male and 793 (77.74%) were female. Of 1020 patients, 224 (21.96%) of patients were hospitalized and 796 (78.03%) were outpatients. Of 224 hospitalized patients, 85% of isolates of E. coli were resistant to ampicillin, while this figure was 90% for K. pneumoniae. Resistant to other antibiotics were also prevalent. Nitrofurantoin was the most effective antibiotics against E. coli and Enterococcus spp. In conclusion, our study revealed that bacterial resistance in uropathogens in our hospital continues to be a great problem and needs drug resistance surveillance periodically.[...] Read More.
Keywords: Urinary tract infections, drug resistance.
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文章
S. A. Balogun* and O. E. Fagade
Abstract: The study reports the diversity of emulsifying bacteria in produce water obtained from Bonny Oil Terminal, Niger Delta, Nigeria. Physico–chemical analysis of produce water showed a high chloride ion content of 650.0 mg/L. Total aerobic mesophilic bacterial count was 8.6 x 106 CFU/mL while the oil utilizing bacterial count was 1.32 x 106 CFU/mL. The predominant bacteria genera were Bacillus, Pseudomonas, Serratia and Klebsiella. The ratio of the surface active agent producers in the mesophilic bacteria was 0.34%, while 2.2% was recorded among the oil utilizing bacteria. The highest emulsification and de-emulsification indices of 65 and 50% recorded respectively were for Pseudomonas mallei. The result obtained was discussed in relation to the use of the emulsifying bacteria in microbial enhanced oil recovery (MEOR) in the Niger Delta, Nigeria.[...] Read More.
Keywords: Emulsifying bacteria, emulsification-index, surface-active agents, biosurfactants, MEOR, crude oil, Niger-Delta.
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A. B. M. S. Hossain* and A. R. Fazliny
Abstract: The world is facing problems of fossil fuels depletion. The problem of using fossil fuels can have an environmental impact due to the oxidation of CO2, SO2 and NOX emission. In order to overcome those problems, there is an alternative fuel that can replace fossil fuel such as bioethanol and biodiesel. Bioethanol can be derived from biomass with many different varieties of feedstocks such as corn, sugarcane, wood and fruits wastes that are renewable and sustainable resources, which are easily accessible and reliable and can help to clean the environment from the wastes. The experiment was conducted to prove that, bioethanol can be produced from rotten pineapples waste through the biochemical reaction called fermentation by commercial yeast, Saccharomyces cerevisiae which is suitable for running petrol engine. The influencing parameters that affect the production of bioethanol from pineapples wastes were optimized. The effects of pH, temperatures, fermentation period, substrate concentration with and without water, components of pineapples from rotten and fresh ones were investigated. From the results, the optimal yield of bioethanol in the parameters such as pH, temperatures, fermentation period, substrate concentrations was found to be 8.7% having pH 4 at 30°C using 3 g/l. The result for viscosity was found under American Society for Testing Materials (ASTM) standard in different concentration of yeast. The anhydrous ethanol was analyzed and it was found that, there was no dangerous element in it’s acceptability as a transportation fuel based on ASTM standard. The elements that were mostly contained in the samples of ethanol production from pineapples wastes were Fe, Cu, Sn, Mn, Ag, Mo, Zn, P, Ca, Mg, Si, Na, B and V, but there was no significant difference among all elements. Key word: Bioethanol, waste pineapple, yeast, temperature, viscosity.[...] Read More.
Keywords: Bioethanol, waste pineapple, yeast, temperature, viscosity.
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文章
Gnagna Diagne-Leye*, Teresa Almaraz-Lopez, Maïmouna Sy-Ndir, Gérard Mascarell, Amadou Tidiane Ba and Christophe Roux,
Abstract: We revisited the systematic position of the fungus Moesziomyces penicillariae, the causal agent of smut of pearl millet, by using morphological characters, germination pattern of teliospores and molecular analysis of ribosomal sequences. Samples of smutted ears of pearl millet were harvested in Senegal (West Africa). Compared to the description of Moesziomyces genus sensus Vánky, our samples presented morphological differences: i) presence of a columella-like structure in sori; ii) surface ornamentations of teliospores; iii) teliospore germination similar to Ustilago and Sporisorium ones. We investigated the systematic position of our samples by aligning their Internal Transcribed Spacer (ITS) sequences of the ribosomal regions with 47 sequences from Ustilaginaceae. The resulting tree rooted with Tolyposporium junci allowed the separation of five groups among which, they are, two Ustilago and two Sporisorium. An independent clade is formed by Tranzscheliella williamsii and Tranzscheliella hypodytes species including Ustilago sparti. Moesziomyces species used in this analysis form a monophylletic group located in Ustilago 2 group, which include different Ustilago and Sporisorium species but also Pseudozyma antartica. Our results indicate the necessity to amend the Moesziomyces genus as the morphological and molecular data confirm that they are included in the Ustilago-Sporisorium complex.[...] Read More.
Keywords: Pearl millet smut, Moesziomyces penicillariae, Ustilaginaceae.
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文章
Nizami Duran*, Yunus Dogramaci, Burcin Ozer, Cemil Demir and Aydiner Kalaci
Abstract: This study was aimed at investigating: (i) three adhesin genes (clf A, fnb A and cna) in Staphylococus aureus strains, (ii) the presence of slime (ica A and ica D genes) in both Staphylococus epidermidis and S. aureus strains isolated from surgical wounds. The slime and adhesin genes were detected by multiplex PCR. The ica A/ica D positivity rates were determined as 66.2% (104/157) in a total of 157 staphylococcal strains. While the occurance rate of slime genes was 69.6% (48/69) among the S. epidermidis, this ratio was 63.6% (56/88) among the S.aureus isolates. No statistically significant difference was found between S. epidermidis and S. aureus isolates in terms of the presence of slime genes (p > 0.05). Among the 88 S. aureus strains, almost all of the strains were positive for fnb A gene (97.7%). The cna and clf A positivity rates were detected in 69 (78.4%) and 45 (51.1%) isolates, respectively. The ica A and ica D genes responsible for slime production have been found to have high prevalence. Also, the frequency of adhesin genes was determined at a high rate in S. aureus strains isolated from surgical wounds. Molecular identification of virulent staphylococcal strains may help in management in clinical decision making.[...] Read More.
Keywords: Adhesins, slime, Staphylococus aureus, Staphylococus epidermidis, wound infection.
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