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Freitas, A. C.*, Silva M. A. R., Jesus, A. L. S., Mariz, F. C., Cordeiro, M. N., Albuquerque, B. M. F. and Batista, M. V. A
Abstract: Bovine papillomaviruses (BPV) are double-stranded DNA viruses that infect the cutaneous and mucosal epithelia inducing hyperplasic lesions in cattle. BPV is the etiologic agent of the papillomatosis and neoplasia of the upper gastrointestinal tract and urinary bladder. The benign and malignant tumors caused by BPV are emergent diseases important for beef and dairy cattle in the world. Although BPV associated tumors have veterinary and agricultural relevance, they have also been studied as a relevant model of human papillomavirus (HPV). Recent studies in BPV biology have shown a great diversity of BPV types and new putative BPV types infecting and co-infecting the herd in several parts of the world. This review will briefly summarize the genomes and structure of BPV and the bovine papillomatosis; will describe in greater detail the genotypic diversity, BPV cross-species infection, relevant aspects of BPV and co-infection and its possible routes of transmission. These new approaches about BPV may be very useful to understand the oncogenic potential of the virus, the relationship between virus and co-factors, and the development of anti-viral vaccines.[...] Read More.
Keywords: Bovine papillomavirus, co-infection, virus transmission, BPV diversity.
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Halit Özkaya, Abdullah Bariş Akcan* and Gökhan Aydemir
Abstract: Mastoiditis is an infection of mastoid process, the portion of the temporal bone of the skull which is behind the ear and contains open air-containing spaces. With possible extra cranial and intracranial complications, acute mastoiditis is the leading complication of acute otitis media (AOM). The goal of this review was to assess the clinical features, pathogens, complications and management of acute and chronic mastoiditis. Retrospective systematic review of studies and articles on acute and chronic mastoiditis between 1983 and 2010 in pubmed, include clinical, epidemiological, microbiological, treatment and outcome data. In 10 studies, the average age was 16, ranging from 6 months to 70 years, with 55% from 0 to 5 years. Most common symptom was otalgia (84 %), 58% of patients had history of past AOM and 61% were under antibiotic therapy during admission. 74% presented with retroauricular swelling and erythema, 58% had a displaced pinna. In general, the prevalence of organisms causing mastoiditis varies greatly between studies, among countries and according to the age of the patient. Reported pathogens were as follows: Streptococcus pneumoniae, most frequently isolated pathogen in acute mastoiditis, prevalence of approximately 25% Group A beta-hemolytic streptococci, Staphylococcus aureus, Moraxella catarrhalis, Haemophilus influenzae, Pseudomonas aeruginosa, Mycobacterium species, Aspergillus fumigatus and other fungi, Nocardia asteroides. Extension of the infectious process beyond the mastoid system can lead to a variety of intracranial and extracranial complications, including meningitis, cerebral abscess, epidural, subdural and intraparenchymal abscesses, vascular thrombosis, osteomyelitis, and abscesses deep within the neck. Permanent damage of the ear leads to hearing loss, vertigo and sometimes facial weakness. The Cochrane review found that antibiotic treatment of otitis media may play an important role in reducing the risk of mastoiditis in populations where it is more common. Despite the use of antibiotics, acute mastoiditis still remains a threat to patients with AOM, especially children under 5 years of age. On the basis of the clinical features and imaging findings, the disease is managed conservatively with intravenously administered antibiotics or treated with mastoidectomy and drainage plus antibiotic therapy. Great care is required by clinicians to make an early diagnosis in order to promote adequate management and prevent complications.[...] Read More.
Keywords: Mastoiditis, otitis media, childhood.
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Renzo F. Martino *, Roberto C. Davicino , María A. Mattar , Yolanda A. Casali , Silvia G. Correa and Blas Micalizzi
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Simphiwe P. Buthelezi, Ademola O. Olaniran* and Balakrishna Pillay
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Sang Phil Shin, Jee Eun Han, Dennis K. Gomez, Ji Hyung Kim, Casiano H. Choresca Jr., Jin Woo Jun and Se Chang Park*
Abstract: The “indo-pacific seahorses”, Hippocampus kuda which had been reared in one of the private commercial aquaria for exhibition were sent to the laboratory for diagnosis. Feces and intestinal contents from 5 seahorses were examined microscopically and revealed numerous scuticociliates. We identified the species of scuticociliate by light (wet mount) and scanning electron microscopy, PCR (polymerase chain reaction) and sequencing. This paper reports the first identification of scuticociliate Philasterides dicentrarchi from seahorse in Korea.[...] Read More.
Keywords: Indo-pacific seahorse, Philasterides dicentrarchi, scanning electron microscopy, polymerase chain reaction, sequencing.
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Monireh Rahimkhani *, Hossein Khavari-Daneshvar and Sara Jamali
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Abdulrahman Ali Mohammed Zabad* , Sultan Ayesh Mohammed Saghir , Anwar Kassem AlMadhagi , Khaled Abdulkarim Al-Moyed
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Hisham N Altayb*, Mohamed A M Siddig, Nagwa M El Amin, Ahmed Ibrahim Hashim Maowia M. Mukhtar
Abstract: This was a cross sectional study conducted to detect and characterize CTX-M genes among extended spectrum β-lactamases (ESBLs) producing Enterobacteriaceae isolated from different regions in Sudan. A total of 305 of Enterobacteriaceae clinical isolates were collected from different regions in Sudan. ESBLs production was initially screened by cefotaxime, cefepime and Ceftazidime, then confirmed by disk combination method and PCR. DNA sequencing was done to differentiate betwen blaCTX-M genotypes. Escherichia coli was the most predominant isolate (58%), followed by Klebsiella pneumoniae (26.6%), Citrobacter freundii (3.6%), Enterobacter species (6.2%) and Proteus species (5.6%). ESBLs were detected by disk combination method in 128/305 (42%) of the tested isolates; Khartoum State 23/36 (64%), Gizera State 54/100 (54%), Sinnar State 49/92 (53%) and White Nile State 2/77 (2.6%). Three quarters of the ESBLs producers (96/128) were positive for blaCTX-M genes by PCR. The blaCTX-M-15 gene was the most predominant gene 18/23 (78.3%), followed by blaCTX-M-14 3/23(13%), blaCTX-M-27 1/23 (4.3%) and blaCTX-M-98 1/23 (4.3%). There was a transition mutation (substitution of A with G at position 25) in the blaCTX-M gene (ID: KP309815), that affected protein structure. In conclusion blaCTX-M-15 was the most commonly encountered gene and widely spread in different Sudanese regions. Keywords: blaCTX-M; Beta-Lactamases Genes; Enterobacteriaceae; Sudan[...] Read More.
Keywords: blaCTX-M; Beta-Lactamases Genes; Enterobacteriaceae; Sudan
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Full Length Research Paper
Abstract: The aim of this study was to investigate Epstein-Barr virus (EBV) serology and EBV DNA in the patients with bone marrow transplantation. In the current study, 128 patients with bone marrow transplantation (BMT) were included in Hematology-Oncology Department of Medical Faculty, Erciyes University between June 2005 and October 2009. In order to evaluate EBV serology and DNA, 357 samples obtained from the patients were studied with enzyme-linked immunosorbent assay and real-time polymerase chain reaction (PCR). The components of EBV serology were anti-Ebstein-Barr nuclear antigen (EBNA)-1 IgG, anti- viral capsid antigen (VCA) IgG and IgM. EBV seropositivity was found in 98.4% of the samples. EBV DNA positivity was detected in 13.3% of the patients. High viral load was only seen in one patient among all the EBV DNA positivity. No patient showed any symptoms regarding EBV virus and admitted with post-transplant lymphoproliferative disease. In conclusion, for the diagnosis and follow up of EBV infections in patients with BMT, detection of EBV DNA by PCR method is also beneficial in addition to serological tests regarding EBV.[...] Read More.
Keywords: Epstein-Barr virus, serological markers, real-time PCR, bone marrow transplant recipients.
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