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Full Length Research Paper
Abstract: The objective of the study was to evaluate mesophyle, psychrotroph and somatic cell counts (SCC), and presence of Staphylococcus aureus in raw milk produced in 42 dairy farms of the State of São Paulo. Farms were classified according to milk yield in low (< 400 L/day, n = 17), intermediate (400 - 1,000 L/day, n = 8) and high milk production (> 1,000 L/day, n = 17). Mesophylic bacteria counts were lower (p < 0.05) in high production farms, with no differences (p > 0.05) in psychrotrophic and somatic cell counts between the three categories. S. aureus was more frequent (p < 0.05) in the milk of individual cows in intermediate production farms. Percentage of samples that did not comply with SCC tolerance limits in grade B milk ranged from 29.4 (low production) to 52.9% (high production). Results indicate that the greatest difficulties for dairy farms to comply with milk quality parameters are related to the adoption of hygiene practices during milking and to failures in milk conservation.[...] Read More.
Keywords: Milk quality, mesophyles, psychrotrophs, somatic cell counts (SCC), Staphylococcus aureus.
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Elendu C. Onwuchekwa*, Uzochukwu G. Ekeleme, Oliver Onu-Osi, Okonudo, I. Diana
Abstract: The prevalence of typhoid fever in referral hospitals in Umuahia and Aba, Abia State, Nigeria was studied using standard microbiological techniques. A total of 500 subjects within the age range of 1-70 years were enrolled. A total of 229 subjects were infected with a prevalence rate of 45.8%. The males had the highest prevalence rate of 50.9%, while females had a prevalence rate of 41.9%. In Aba, 125 (50.0%) subjects were positive, while 104 (41.6%) were recorded in Umuahia. The age group of 21-30 years had the highest rate of 63.3%. Traders were most affected (62.5%).  The stool cultures from males had the highest prevalence of 24.8% in ABSUTH, Aba while females had the lowest of 16.0% in FMC, Umuahia. Hence, health care personnel should not totally depend on widal test alone for the diagnosis of enteric fever but should use other diagnostic methods to differentiate Salmonella infection from other related infections.[...] Read More.
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S. Saraswathi*, V. Vasanthabharathi, V. Kalaiselvi and S. Jayalakshmi
Abstract: A bacterium producing agarase was isolated from the vellar estuary and potential strain was identified as Bacillus subtilis. The ideal parameters was found to be of pH 8, temperature of 40°C and 3% of NaCl concentration for optimal growth and agarase activity. Agar as carbon source and yeast extract as nitrogen source was found to be suitable for optimum growth and agarase activity. The activity of enzyme obtained from the cell free filtrate was 15.2 U/L and the activity of the partially purified enzyme was 5.3 U/L and the purified enzyme activity was found to be 2.3 U/L. The stability of the partially purified enzyme on pH profile was found to be pH 8 and thermostability was found to be up to 40°C. The purified enzyme was determined to be homogeneous on the basis of SDS PAGE and had a molecular weight of 20 KDa.[...] Read More.
Keywords: Agarase, Bacillus subtilis, optimization, stability, SDS PAGE.
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Siriporn Okonogi*, Waranee Prakatthagomol, Chadarat Ampasavate and Srikanjana Klayraung
Abstract: The aim of this study was to explore the killing kinetics and bactericidal mechanism of action of A. galanga against food borne bacteria in order to promote this plant as a source of natural preservative. The comparison on antibacterial power was firstly done among its essential oil and the crude extracts obtained from various extracting solvents. The essential oil showed the extremely strongest antibacterial activity and more effective killing activity against Gram negative than Gram positive food borne bacteria. The kinetic time of the oil for complete bactericidal action against E. coli was 40 min whereas that of gentamicin was 120 min. The scanning electron microscopy (SEM) of bacterial morphology after exposed to the oil showed bacterial membrane destruction. It was concluded that the essential oil is the highest potential part of A. galanga against food borne bacteria. Its killing kinetics against Gram negative bacteria was extremely faster than a broad spectrum antibiotic gentamicin. Its mechanism of bactericidal action was along with the bacterial membrane disruption and malfunction leading to cell death.[...] Read More.
Keywords: Alpinia galanga, antibacterial, mechanism of action, essential oil, killing kinetics.
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Payam Haghighi-Khoshkhoo*, Gita Akbariazad, Masood Roohi, Javad Inanlo, Mehran Masoumi and Pedram Sami-Yousefi
Abstract: This survey was carried out to determine the seroprevalence of Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) infections in commercial layer farms in Centernorth of Iran. A total of 2000 serum samples were collected from 40 commercial layer flocks (50 samples/ flock) mostly > 40-week-old. Sera tested by serum plate agglutination (SPA) method using commercial MG antigen and MS antigen. Positive reactions retested by SPA on 1:8 dilution and the flocks with more than 10% positive reactions considered positive serologically. The results showed that 4 of 40 (10%) flocks were MG positive and the rest (90%) were negative. About MS, 17 of 40 (42.5%) flocks were positive and 33 of 40 (57.5%) were negative. Sera tested for MG were: 125 of 2000 (6.25%) positive, 1821 of 2000 (91.05%) negative and 54 of 2000 (2.7%) suspect. Sera for MS were: 371 of 2000 (18.55%) positive, 1617 of 2000 (80.85%) negative and 12 of 2000 (0.6%) suspect. Statistical analysis showed a positive correlation between MS positive flocks and strain (LSL flocks compared to Hy-Line flocks) and capacity of the farms significantly (P[...] Read More.
Keywords: Mycoplasma gallisepticum, Mycoplasma synoviae, seroprevalence, serum plate agglutination test.
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Shahina Mumtaz, Jawad Ahmed*, Liaqat Ali and Hamid Hussain
Abstract: Infections caused by ESBL producing members of the enterobacteriaceae have rapidly increased all over the world. ESBL increase the possibility of failure of empiric antimicrobial regimens. The aim of this study was to determine the prevalence of ESBL in bacterial isolates and to look into the options for treating infections caused by these organisms. A total of 4,150 isolates of enterobacteriaceae were studied. ESBL producer isolates were 371 (8.94%) out of which 281 (75.7%) were recovered from admitted patients while 90 (24.3%) were recovered from outdoor patients. ESBL detection was carried out according to Clinical Laboratory and Standard Institute (CLSI) criteria. Majority of the ESBL producing isolates were obtained from urine 282 (76.0%), followed by swabs 69 (18.6%) fluids 12 (3.2%) blood 06 (1.7%) and sputum 02 (0.5%). The ESBL phenotype was detected in 322 (89.5%) of the isolates of E. coli, 20 (5.4%) of Klebsiella spp. 14 (3.8%) Enterobacter and 05 (1.3%) Citrobacter spp. Carbapenems was the drug of choice for serious infection with ESBL – producing organisms in Peshawar. These should not be administered as empiric therapy, because their over use can result in significant resistance in future.[...] Read More.
Keywords: Extended spectrum beta lactamases (ESBL), cephalosporins, Escherichia coli, phenotype screening.
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Nevin Sanlier*, Ayhan Dağdeviren, Bülent Çelik, Saniye Bilici and Aktolkin Abubakirova
Abstract: Complete and balanced nutrition with reliable food consists of the basis of health and protective health services. Therefore, the current study was carried out to determine the knowledge of food safety level and purchasing behavior of 668 consumers living both in Turkey (n=348) and in Kazakhstan (n=320) and to compare the results. Volunteered consumers for the research were given a face to face interview between March and September 2010. It was found that the knowledge of purchasing behavior (14.43±2.56) of food safety (20.82±4.20) of the consumers living in Turkey was higher compared to those living in Kazakhstan (11.84±2.92, 14.74±3.86 respectively) and that the difference between the two countries was statistically significant (p[...] Read More.
Keywords: Purchasing behavior, knowledge of food safety, consumer, Turkey, Kazakhstan.
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S. M. E. Rahman, Joong Hyun Park and Deog-Hwan Oh*
Abstract: The study was designed to investigate bactericidal and fungicidal actions of salicid (pH: 6.7, ORP: 760 mV, residual chlorine of 2 to 5 ppm) on hospital infections. Four of the most common opportunistic pathogens (Klebsiella pneumoniae, Staphylococcus aureus, Rhizopus oryzae, and Aspergillus fumigatus) were used for this study. Cultures were inoculated in 9 mL of salicid and incubated for 0.5, 1, 3, 5, 7 and 10 min at room temperature (23 ± 2°C). A dipping method was followed for this study. Untreated pathogens were treated as control. Compared to the untreated control, a reduction of 1.10 to 6.08 log10 CFU/mL for aforementioned pathogens were recorded as the result of treatment with 2 and 5 ppm salicid, respectively. The highest bactericidal effect was found with S. aureus for 5 ppm salicid with 0.5 min immerse time. Salicid treatment with 0.5 min dipping and 5 ppm residual chlorine also reduced K. pneumoniae, R. oryzae, and A. fumigatus by 5.00, 3.50 and 2.63 log10 CFU/mL, respectively. Our findings showed that in each pathogen, efficacy of salicid decreased significantly (p < 0.05) with increased dipping time, from 0.5 to 10 min and there was significant difference (p < 0.05) observed between 2 and 5 ppm salicid treatment in reducing pathogens. The results indicate that salicid may be a useful disinfectant for hospital infections, but its clinical application has still to be evaluated.[...] Read More.
Keywords: Salicid, bactericidal and fungicidal effect, hospital infections.
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Oladunmoye, M. K.* and Kehinde, F. Y.
Abstract: Ethnobotanical survey of plants used to treat some common viral diseases, such as chicken pox, poliomyelitis, influenza, hepatitis, measles and jaundice was carried out in Ekiti, Ondo, Osun and Oyo States in South Western Nigeria. Questionnaires were distributed among the participants and oral discussions were employed. Two hundred and eight (208) data were collected and tabulated. The Yoruba names, botanical names, methods of preparation and mode of administration of the plants were considered. The research work showed that members of the family Annonaceae (10.3%) were most frequently used, followed by members of Leguminosae (9.9%), and Zingiberaceae (7.9%), for treating chickenpox and measles infections, although all other plants play prominent roles in peoples` health care. Also, knowledge of medicinal herbs was being left in the hands of the elders between the age range of 51-70 years and 71-80 years. The leaves of the plants (45.5%) were mostly used in treating viral infections, followed by the use of stem bark (13.5%). All other parts were less commonly used to treat viral diseases. Mono-prescription was rare in the data collected. Oral application had the highest mode of administration (83.7%), while the combination of both oral and external applications (12.5%) closely followed. Decoction (90.4%) was the most frequently used method of anti- viral herbal preparation, while concoction (0.9%) was least used. Herb sellers (72.6%) constituted the major source of information about the use of anti-viral medicinal herbs, followed by traders, civil servants and herbalists with 17.31, 6.25 and 3.85 respectively. Many plants in the studied area may be a good source of lead molecules needed in viral chemotherapy after extraction of the bioactive components as well as removal of toxic residue following toxicological studies.[...] Read More.
Keywords: Leguminosae, mono-prescription, chickenpox, decoction.
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文章
Yogyata Marothi* and Binita Singh
Abstract: This study was conducted to determine the prevalence of intestinal parasites in hospitals at Ujjain. A total of 5990 samples were collected in five years: 3580 from Ujjain Charitable Trust Hospital (Urban population) and 2410 from R. D. Gardi Medical College (Rural population). Overall prevalence rate of intestinal parasite was 21.4%. Entamoeba histolytica (10.5%) was the commonest protozoa followed by Giardia lamblia (3.9%). Among the helminths, Ascaris lumbricoides (2.8%) was the commonest. Multiple infections were seen in 70 samples. There was difference in prevalence between urban (20.2%) and rural (23.1%) population. Females (27.4%) were more affected than males (18.2%) and age group 0 to 10 years old had the highest rate of infection. The results indicate that there is a need to implement control measures in form of regular deworming, health education and provision of safe water supply.[...] Read More.
Keywords: Intestinal parasites, prevalence, rural, urban, Ujjain.
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