MOSP - Macao Scientific Publishers

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Does borna disease virus (BDV) have a role in the etiopathogenesis of schizophrenia?

Karakose A. R., Yuksel P., Turan N., Ziver T. , Sariba S., Nihat Alpay, Balcioglu I., Aslan M., Helps C. R., Yilmaz H. and Kocazeybek B.*

Abstract: In human, certain serological and molecular trials estimated an association between psychiatric diseases, particularly schizophrenia, mood disorders and borna disease virus (BDV). The purpose of this study was to investigate whether BDV has association with etiopathogenesis of schizophrenia. A total of 207 patients who were diagnosed with residual schizophrenia and 51 patient control grop with 134 healthy control group were included in the study. RNA of borna disease virus (BDV p24 and p40) in the peripheral blood was investigated by using real-time RT-PCR. A highly significant difference was detected between patient and healthy control group cases in terms of BDV IgG seropositivity (p[...] Read More.

Keywords: Borna disease virus, schizophrenia, ELISA.

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A recombinant E0 gene of bovine viral diarrhea virus protects against challenge with bovine viral diarrhea virus of sika deer

Yugang Gao, Yajun Wang, Rui Du, Quankai Wang, Pengju Zhang*, Shuzhi Wang and Lianxue Zhang*

Abstract: Eukaryotic expression plasmid (PVAX 1/E0) of E0 gene was constructed from an isolated strain of bovine viral diarrhea virus (BVDV) from sika deer and transfected into the BHK-21 cells to detect E0 gene transcription expression level and protein expression level. It was found that E0 gene could express in BHK-21 cells by RT-PCR and ELESA results. The humoral and cellular immune response levels were detected in rabbits immunized with PAVX1/E0 and inactivated vaccine by ELESA technology, which reached the peak after 42 days immunization and PVAX1/E0 immune rabbits could produce specific higher cells immune responses level in comparison to inactivated vaccine group. This is the first report demonstrating and “immunogenicity” of BVDV E0 gene on “sika deer”, indicating a new vaccine target for BVDV.[...] Read More.

Keywords: Sika deer, bovine viral diarrhea virus, E0 gene, eukaryotic expression, immune response.

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Production of recombinant cellulase enzyme from Pleurotus ostreatus (Jacq.) P. Kumm. (type NRRL-0366)

Ayman S. Daba, Ghada A. Youssef*, Sanaa S. Kabeil and Elsayed E. Hafez

Abstract: Successful utilization of Pleurotus ostreatus (Jacq.) P. Kumm. (type NRRL-0366) mushroom as a type of edible locally isolated mushroom in Egypt at the Mushroom Research Center (Mubarak City for Scientific Research and Technology Applications), to produce extensive hydrolyzing cellulase complex enzymes. This hydrolysis was approached in submerged culture supplemented with avicel PH101 as a substrate for endo-,exoglucanase production. The avicel concentration 6% yielded the maximum enzyme activities (2.46, 1.80 U/ml) for both endo-and exoglucanase activities on basal medium at 27°C, initial pH value of 5.5 for 12 days on rotary shaker (180 rpm) incubation period. Cellulase enzyme was amplified using specific PCR and the amplicone was cloned using TOPO TA cloning vector. The cellulolytic activity of the recombinant protein was examined and high activity was obtained compared with the standard ones. The avicel was used as a sole carbon source in the fermentation medium and the results revealed that, avicel induced the cellulolytic activity of the examined organism compared with those grown on medium deficient of avicel.[...] Read More.

Keywords: Pleurotus ostreatus, mushroom, avicel, recombinant cellulolytic enzymes.

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Screening and induction of laccase activity in fungal species and its application in dye decolorization

Vaidyanathan Vinoth Kumar, Selvaraj Dinesh Kirupha, Premkumar Periyaraman and Subramanian Sivanesan*

Abstract: A great deal of works has been carried out assessing dye-decolorization capabilities of fungi, but only few species were investigated. In this present study, ten fungal species were screened for laccase activity by indicator plate method, of which five species were found to be laccase-positive. Laccase activity varied during growth and maximal laccase activity was observed during the 9th day, except for Agaricus bisporus. Pleurotus ostreatus gave the highest laccase activities, showing average value around 570 U/L, which were higher than other strains. Addition of 1 mM copper sulphate induced the laccase production efficiently by 60 to 80%, while veratryl and benzyl alcohol induced laccase production in all the laccase positive species except for A. bisporus. Constitutive expression of laccase was observed in F. solani in the presence of copper sulphate. In addition, the extracellular laccase from the P. ostreatus could decolorize reactive dyes, which suggests the potential application of laccase in textile effluent treatment.[...] Read More.

Keywords: Dyes, decolorization, inducer, laccase, Pleurotus, textile.

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Variability in antimicrobial activity of latex from two varieties of Ficus carica

Houda Lazreg Aref*, Bel Hadj Salah Karima, Abdelwaheb Fekih, Rachid Chemli, Massoud Mars, Mahjoub Aouni, Jean Pierre Chaumon and Khaled Said

Abstract: Fig latex has been a typical component in the health -promoting Mediterranean diet for millennia. To study its potential constituents, two varieties differing in color of fruit [Bidhi Bither: (BB); Kahli Bither: (KB)] and their growth area were analyzed for their organic extracts against sixteen microbes. The capacity of extracts was evaluated based on the inhibition zone, using the disc-diffusion assay, minimal inhibition concentration (MIC) for bacteria and yeasts, the method of calculating the inhibition percentage (I%) and Agar punched wells method for fungi, respectively. Extracts of (BB1) variety from Chott Meriam showed the highest antimicrobial activities against the studied microorganisms when compared to (KB) variety and those from Mahdia. Ethyl acetate extracts had inhibition effect on the multiplication of Enterococcus faecalis, Citobacter freundii, Pseudomonas aeruginosa, Proteus mirabilis and Escherchia coli. For the opportunist pathogenic yeasts, ethyl acetate and chloroformic fractions showed a very strong inhibition (100%) and an MIC of 0.082 and 1.25 µg/ml for (BB) and (KB), respectively, while ethanolic fraction had a total inhibition against Candida albicans, Candida glabrata, Candida krusei and Candida parapsilosis. Microsporum canis was strongly inhibited with ethanolic extract (75%) and totally with ethyl acetate extract at a concentration of 500 µg/ml.[...] Read More.

Keywords: Ficus carica, latex, organic extracts, fungi, yeasts, bacteria, antimicrobial activity.

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Effect of some culture substrates (date-palm peat, cocopeat and perlite) on some growing indices and nutrient elements uptake in greenhouse tomato

Ahmad Mohammadi Ghehsareh*, Hassan Borji and Mehrdad Jafarpour

Abstract: In recent years, a wide range of soilless culture techniques have been developed and commercially introduced for intensive production of horticultural crops, particularly in greenhouses. Reasons for replacing soils as growing media arise from plant protection problems with soil-borne pathogens and environmental regulations against groundwater pollution with nitrate and pesticides. The aim of this study was to compare some growing indexes of greenhouse tomato that were cultivated in some substrates, such as perlite, date-palm peat and coco peat. The research was conducted in a completely randomized design with 6 replications. The treatments were coco peat + perlite (v/v=50%)‚ date-palm peat + perlite (v/v=50%)‚ perlite (100%) and date-palm peat(100%). Papadopolus formula was used for nutrient solution during plant growth with fertigation method. Also temperature, humidity and irrigation rate was constant for all treatments. Comparison of means showed that the media had no significant effect on concentration of nutrient elements in fruit such as N, P, K and yield in all treatments. Minimum and maximum amount of fruits yield was in date-palm peat and perlite treatments respectively. That had no significant difference with other treatments. Higher amount of total soluble solids (TSS) related to coco peat + perlite treatment that has not any significant difference with date-palm peat + perlite, perlite and date-palm peat treatments. Also, culture substrates had no significant difference on the amount of ascorbic acid. The results showed that date-palm peat is an appropriate media for soilless culture with suitable physical and chemical properties, availability and low cost. Therefore, it can be a new substrate that is introduced for replacing other media. Key word: Soilless, substrate, date-palm peat, total soluble solids, ascorbic acid.[...] Read More.

Keywords: Soilless, substrate, date-palm peat, total soluble solids, ascorbic acid.

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Antibacterial activity of ZnO nanoparticle on gram-positive and gram-negative bacteria

Zarrindokht Emami-Karvani* and Pegah Chehrazi

Abstract: The aim of the present study is to determine the antimicrobial activity of ZnO nanoparticles against Gram-negative and Gram-positive bacteria. Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were used as test microorganisms. The effects of particle size and concentration on the antibacterial activity of ZnO nanoparticles was studied using bacteriological tests such as disc and well diffusion agar methods, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). These tests were performed in nutrient broth and nutrient agar following standard methods. In addition, the effect of different concentrations of ZnO nanoparticles on the growth of E. coli and S. aureus was measured with respect of time. The minimum inhibitory concentration was determined using seven different concentrations of ZnO nanoparticles including 16, 8, 4, 2, 1 and 0.5 mg/ml. The MIC value for E. coli and S. aureus was 1 and 0.5 mg/ml, respectively. The results showed that ZnO nanoparticles have antibacterial inhibition zone of 29 and 19 mm at the concentration of 10 mg/ml against E. coli and S. aureus, respectively. Gram-negative bacteria seemed to be more resistant to ZnO nanoparticles than Gram-positive bacteria. It was found that the antibacterial activity of ZnO nanoparticles increased with decreasing particle size and increasing powder concentration. The antibacterial effect of ZnO nanoparticles was time dependent and takes effect gradually. ZnO bulk powder showed no significant antibacterial activity. Key word: ZnO nanoparticle, Escherichia coli, Staphylococcus aureus, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC).[...] Read More.

Keywords: ZnO nanoparticle, Escherichia coli, Staphylococcus aureus, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC).

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Biodegradation of 2,2,6,6-Tetramethyl-4-piperidone by three soil bacteria strains and their isolation and identification

Q. F. Li*, L. F. Wang, C. J. Hao, G. M. Zhao and J. H. Wang

Abstract: In order to isolate and characterize 2,2,6,6-Tetramethyl-4-piperidone (TMPD)- degrading bacteria and to select the optimal degrading conditions, different bacterial types which can degrade TMPD were isolated from soil samples. Three strains (LF-1, LF-2 and LF-3) showed strong biodegradation ability by UV-visible spectrophotometer analysis. Morphological and biochemical analyses, as well as 16S rRNA gene sequence comparisons of three strains were further carried out. They were identified as Klebsiella oxytoca, Staphylococcus pasteur and Bacillus flexus, respectively. K. oxytoca, S. pasteur and B. flexus had degrading capabilities for TMPD in mineral salt medium of 80.9, 69.8 and 64.9%, respectively, during 24 days of incubation. Maximum degrading rates were obtained by an initial concentration of the TMPD of 200 mg/L, an incubation temperature of 30°C, and constant aeration (180 rev/min). In conclusion, K. oxytoca, S. pasteur and B. flexus show their potentials in TMPD detoxifying and in protecting the TMPD contaminated environment.[...] Read More.

Keywords: 2,2,6,6-Tetramethyl-4-piperidone (TMPD), biodegradation, Klebsiella oxytoca, Staphylococcus pasteur, Bacillus flexus.

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Potential of Beauveria bassiana as a biological control agent against two important and destructive stored pestsâ€™ C. maculatus and S. granarius adults

Full Length Research Paper

Abstract: This research was carried out to evaluate the suppressive efficacy of entomopathogenic fungi Beauveria bassiana against adults of Callosobruchus maculatus (F.) and Sitophilus granarius (L.) on stored grains in darkness (27±2°C and 65±5% RH) . The insects were inoculated by following immersion in five aqueous suspensions with concentrations determined with preliminary tests and compared with untreated wheat and cowpea as control. Probit analysis showed that the lowest LT 50 values in suspensions with highest concentrations (2.3 × 107 conidia ml-1) were 6.63 and 10.45 days for C. maculatus and S. granarius, respectively. On the other hand, the LC50 values on day 9 post-treatment were 3.17 × 106 and 6.08 × 107 con.ml-1 for C. maculatus and S. granarius, respectively. Comparison of Lc50, LT 50 values and mortalities indicated that in both assays, B. bassiana was consistently more virulent for C. maculatus than S. granarius. The overall results showed that these two pests were controlled successfully with entomopathogenic fungi Beauveria bassiana.[...] Read More.

Keywords: Callosobruchus maculatus, Beauveria bassiana, Sitophilus granarius, stored grain.

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Optimization of bacteriocin production by Lactobacillus plantarum YJG, isolated from the mucosa of the gut of healthy chickens

Bing Han, Zhanqiao Yu, Baosheng Liu, Qingshan Ma and Rijun Zhang*

Abstract: The objective was to develop an optimal, albeit low-cost medium for bacteriocin production, thereby facilitating industrial production. Soybean meal and peptide, two low-cost nitrogen sources often applied in industrial fermentation, were used to replace their expensive counterparts in De Man Rogosa and Sharpe (MRS). Two factors were first chosen from the 11 considered in the Plankett-Burman (PB) design. Then, the path of steepest ascent and central composite design (CCD) were used to approach the optimum region of the response and determine the maximum activity of the bacteriocin. Optimal concentration of glucose (36.3 g/l) and NaCl (1.41 g/l) stimulated the production of bacteriocins. And the optimal equation was then verified by 50 L fermentor. Under optimized conditions, Lactobacillus plantarum YJG produced a 1.4 fold higher production of bacteriocin than the common MRS, with 40.6% cost savings relative to non-optimized conditions.[...] Read More.

Keywords: Lactobacillus plantarum YJG, bacteriocin, response surface methodology, central composite design, optimization.

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