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Camila O. Vilela, Geferson Fischer*, Clarissa C. de Castro, Cristina F. Nunes, Silvia O. Hübner, Margarida B. Raffi, Simone E. Salles, Marcos A. Anciuti and Gilberto D. Vargas
Abstract: Recent pandemics caused by virus like influenzavirus (H1N1, H5N1) reaffirm the importance of studies aiming at obtaining new virucidal and/or antiviral substances, once its prolonged use can lead to resistance to the active principles. Green propolis, which has several scientifically proven bioactive properties, was evaluated in this study as an ethanol extract regarding its virucidal capacity against avipoxvirus (APV) inoculated in chorioallantoic membrane of chicken embryos (CAM). Eggs inoculated with virus and 2400 µg/dose of propolis, previously incubated for four hours, presented reduction in the pox lesions number (p[...] Read More.
Keywords: Green propolis, virucidal activity, avipoxvirus, chorioallantoic membrane.
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Badrul S. M. Zainuddin*, Mustaffa Musa and Zainul F. Zainuddin
Abstract: We have developed a recombinant Salmonella typhi Ty21a vaccine candidate (rSTvacIII) displaying a multi-epitopes mycobacterial antigen called the VacIII (containing ubiquitin and 4 immunogenic mycobacterial epitopes of ESAT-6, PhoS1, Hsp 16.3 and MTB 8.4 genes) on the surface of S. typhi Ty21a (Ty21a) using PgsA protein as a carrier. The expression of VacIII protein on the surface of Ty21a was verified by western blot. Immunization in BALB/c mice with rSTvacIII induced strong Th1-type responses based on high level of in vitro IFN- but low IL-4 secretion. Since the protective immunity against tuberculosis is mediated by Th1-type response, the rSTvacIII have potential as effective vaccine candidate against tuberculosis.[...] Read More.
Keywords: VacIII antigen, PgsA anchoring motif, tuberculosis, Salmonella typhi Ty21a.
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Akuodor G. C.*, Onyewenjo S. C., Anyalewechi N. A., Essien A. D. Akpan J. L., Okoroafor D. O. and Okere M. O.
Abstract: Antimicrobial properties of Berlina grandiflora Hutch. and Dalz. leaf extract were investigated against both clinical and laboratory isolates. Susceptibility of these isolates to the extract was determined using disc diffusion method. The antimicrobial screening had wide range of activity on Escherichia coli, Stapylococcus aureus and Streptococcus spp. The diameter of zone of inhibition by the extract was 7, 8.7 and 9 mm respectively. The minimum inhibitory concentrations (MIC) were 32.81, 19.38 and 11.72 µg/ml for E. coli, S. aureus and Streptococcus spp. Based on the current findings, it can be concluded that B. grandiflora has antimicrobial activity against certain microorganisms.[...] Read More.
Keywords: Berlina grandiflora, antimicrobial agent, minimum inhibition concentration, in vitro.
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Tao J., Zhu l. Q., Lin Y. Q. and Zhu G. Q.*
Abstract: Eighty calf blood samples were collected from different areas of China and were examined. Sixteen non cytopathic isolates of bovine viral diarrhea virus genotype 2 (BVDV-2) were isolated by specific BVDV-2-directed RT-PCR and sandwich enzyme-linked immunosorbent assay (ELISA). The results were verified by the indirect immunofluorescence assay and the ultra-thin sections. Genes of 5'-UTR, E2 and Npro from all the 16 field isolates were sequenced. The sequence identities at the nucleotide and amino acid levels were higher than 99.0%. The phylogenetic analysis revealed that the BVDV-2 USA strain, AzSpin, had the highest sequence homology with each of the Chinese isolates.[...] Read More.
Keywords: Bovine viral diarrhea virus genotype 2 (BVDV-2), isolation and characterization, phylogenetic analysis.
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Full Length Research Paper
Abstract: Corn cob hydrolysis with 1% (v/v) of sulfuric acid yielded 36.49 total reducing sugars (g/l) along with various fermentation inhibitors such as fufural, phenolics compounds and acetic acid. The acid hydrolysate detoxified with overliming plus activated charcoal brought about a maximum decrease in fufural (100%), acetic acid (62.4%) and phenolics compounds (96.6%). Treatment of hydrolysate with overliming caused 99, 51.4 and 16.7% loss in fufural, acetic acid and phenolics compounds, respectively. Fermentation by Candida shehatae ACCC 20335 showed a maximum yield of ethanol (0.31 g/g) and a productivity of 0.152 g/l/h with overliming plus activated charcoal treated hydrolysate.[...] Read More.
Keywords: Corn cob, detoxification, ethanol, fermentation, hydrolysate.
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Kai Zhong, Xia-Ling Gao , Zheng-Jun Xu*, Li-Hua Li, Rong-Jun Chen Xiao-JianDeng, Hong Gao, Kai Jiang, and Isomaro Yamaguchi
Abstract: In the present study, the phylogenetic analysis of the Streptomyces strain Eri11 isolated from the rhizosphere of Rhizoma Curcumae Longae and the antioxidant activity of the broth cultured with Eri11 were investigated. Analysis of 16S rDNA gene sequences demonstrated that the strains Eri11 was most closely related to representatives of the genera Streptomyces. The total phenols and flavonoids contents in cultured broth were detected to be13.59 ± 0.17 mg gallic acid equivalent/g and 9.93 ± 0.83 mg rutin equivalent/g, respectively. The cultured broth showed the antioxidant activity against the ABTS (2, 2’-Azinobis-3-ethyl benzthiazoline-6-sulfonic acid) free radicals and hydroxyl free radicals with IC50 (The half-inhibitory concentration) of 223.81 ± 24.50 μg/ml and 582.42 ± 83.10 μg/ml respectively. So, it was suggested that the isolated Streptomyces strain Eri11 could be a candidate for the nature resource of the antioxidants.[...] Read More.
Keywords: Streptomyces, phylogeny, cultured broth, antioxidant activity.
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J. Akbarmehr
Abstract: Ringworm is a fungal and zoonotic infectious disease, caused by different species of dermatophytes. Lesions of ringworm are usually found on the head, muzzle, ears, neck, and particularly, around the eyes of the infected animals. This study was conducted to determine the prevalence of cattle ringworm in native farms of Sarab city in Iran. A total number of 1150 cattle in native dairy farms of Sarab city were examined in this study from July 2007 till June 2008. The animals were classified into two age groups of less than 2 years and more than 2 years. Each animal was thoroughly examined for skin ringworm lesions, then, microscopic and culture examinations were carried out on samples obtained from ringworm lesions of infected animals. According to the results of this study among 1150 cattle, 188 of them (16.34%) were clinically positive for skin ringworm lesions. After microscopic and culture examinations, 150 animals (13.04%) were confirmed for dermatophyte infections. Trichophyton verrucosum was isolated in 100% of positive samples. The prevalence of ringworm among cattle less than 2 years and more than 2 years were 15 and 9% respectively, which showed significant difference between them. This study is the first research on the prevalence of cattle ringworm in Sarab city which indicated the relatively high prevalence of the disease and revealed the dominant role of T. verrucosom in cattle ringworm.[...] Read More.
Keywords: Ringworm, dermatophyte, cattle, Sarab.
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Uzoigwe C. I.* and Agwa O. K.
Abstract: The aqueous ethanol and acetone-ethanolic extracts of Vernonia amygdalina leaves and stems were investigated for their antimicrobial activities compared to ciprofloxacin, against Escherichia coli, Klebsiella sp and Staphylococcus sp. The antimicrobial activity of each extract on the isolates was determined by agar well diffusion and minimum inhibitory concentration methods. The acetone-ethanolic extract of the leaf showed the highest antibacterial activity on Klebsiella sp in both methods used, followed by the ethanol leaf extracts on the same organism. E. coli and Staphylococcus sp were not susceptible to any of the extracts even at higher concentrations. The aqueous extracts of both leaf and stem were ineffective on all the test isolates at all concentration. Comparatively, the test isolates were sensitive to ciprofloxacin in both methods used but the antibacterial effect of acetone- ethanolic leaf extract on Klebsiella sp was higher than that of ciprofloxacin on Klebsiella sp. The results from this research showed that V. amygdalina could be used as raw material in the synthesis of antimicrobial drugs, especially against Klebsiella sp which have been attributed with high level drug resistance in urinary tract infections.[...] Read More.
Keywords: Ciprofloxacin, acetone, urinary tract infections, ethanol.
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J.  Nzungize*, P. Gepts, R. Buruchara, S. Buah, P. Ragama, J. P. Busogoro and J. P. Baudoin
Abstract: A series of 231 samples of bean plants affected by bean root rot were collected from different areas of Rwanda in order to characterize the causal agents. The collected samples were used to isolate 96 typical Pythium colonies which were classified into 16 Pythium species according to their respective molecular sequences of the ribosomal ITS fragments. Inoculation assays carried out on a set of 10 bean varieties revealed that all identified species were pathogenic on common bean. However, the bean varieties used in this investigation showed differences in their reaction to inoculation with the 16 Pythium species. In fact, the varieties CAL 96, RWR 617-97A, URUGEZI and RWR 1668 were susceptible to all the Pythium species while the varieties G 2331, AND 1062, MLB 40- 89A, VUNINKINGI, AND 1064 and RWR 719 showed a high level of resistance to the all Pythium species used in our study. This high level of resistance to Pythium root rot disease found in diverse varieties of common bean grown in Rwanda constitutes a real advantage to be exploited as source of resistance in breeding programs aiming to increase resistance to the disease in the most popular bean varieties grown in Rwanda.[...] Read More.
Keywords: Bean, characterization, molecular, Phaseolus, Pythium, root rot.
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文章
J. Akbarmehr* and M. Ghiyamirad
Abstract: Brucellosis is an infectious and zoonotic disease of worldwide distribution. Despite its control program, the disease is endemic in Iran and remains one of the most important public health problems. The aim of this survey was to determine the seroprovalence of brucellosis in livestock animals in Sarab City, Iran. A total of 1500 animals (600 cattle, 740 sheep and 160 goats) were examined for brucellosis from February 2007 to September 2008. The examined animals were divided into two sex groups (male and female). Moreover cattle were divided into four breed groups (Holstein, Brown Swiss, Native and Mixed). Serological examinations including Rose Bengal plate test (RBPT), serum agglutination test (SAT) and 2-mercaptoethanol test (2ME) were performed on serum samples obtained from examined animals. In overall, out of 1500 blood samples 61 (4.06%) were positive for brucellosis. The prevalence of brucellosis in cattle, sheep and goats were found 3.66, 4.18 and 5%, respectively. The prevalence rates of brucellosis in different breeds of cattle, Holstein, Brown Swiss, native and mixed breeds were determined as: 4.72, 2.22, 2.50 and 3.75%, respectively. The prevalence rates of the disease in male and female animals were determined as follows: Male cattle, 1.53%, female cattle, 3.92%, male sheep, 2.8%, female sheep, 4.89%, male goats, 2.22%, and female goats, 6.08%. There were differences in the prevalences of brucellosis in different breeds and sexes of examined animals however statistically were not significant (P>0.05, X2[...] Read More.
Keywords: Brucellosis, Sarab, prevalence, livestock.
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