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Yavar Sharafi*, Jafar Hajilou, Seyyed Abol-Gasem Mohammadi and Mohammad Reza Dadpour
Abstract: Most of the Iranian almond cultivars are self-incompatible followed by pollination, fertilization, fruit set and lower yield problems. Therefore, selecting suitable cross-compatible cultivars for orchard establishment is necessary especially by new cultivars/genotypes obtained from breeding programs. In this study fruit set and pollen tube growth of ten late- bloom almond genotypes, obtained from a breeding program (D, E, F, I, G, L, K, O, P and Q) were investigated under filed and lab controlled pollination conditions. In order to study self-and cross-(in) compatibility they were pollinated by the pollens of overlap blooming-time genotypes in both conditions. Initial and final fruit set, fruit drop and some of the kernel traits were measured under filed condition. Measurements of pollen tubes at the style and in ovary were scored using fluorescence microscopy in lab, Results showed significant differences in some of the studied characters among crosses in both methods and Results confirmed each other in both methods. Fruit set percentage and pollen tube number in the ovary demonstrated that, all of the genotypes were self-incompatible but cross- (in) compatibility was not observed among them. In conclusion all of the genotypes could be used as a suitable polinizers for each others, regarding overlapping blooming-time of genotypes.[...] Read More.
Keywords: Almond, self-incompatibility, cross-incompatibility, fruit set, fluorescence microscopy.
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文章
Rohit Sharma*, Rakesh K. Baghel and Akhilesh K. Pandey
Abstract: Ectomycorrhizal mushroom Cantharellus tropicalis was grown in axenic culture to study the effect of pH, temperature, nitrogen, carbon, phosphorus and heavy metals/trace elements on production of acid phosphatase and mycelial growth. The results of present study showed optimum mycelial growth with pH 4 at 15 and 35 ± 2°C. The ectomycorrhizal mushroom mycelia utilized lactose and yeast extract as best carbon and nitrogen source for biomass production. Ferrous sulphate supported the maximum mycelial growth when different trace and heavy metal were used. Among phosphorus sources, di-Sodium hydrogen phosphate supported maximum growth. The acid phosphatase production did not follow a uniform pattern as inferred from observations in this study. Moreover, results showed that high biomass did not mean more acid phosphatase production. However, pH 5 at temperature 15 and 35 ± 2°C supported high enzyme production. Fries Das medium supplemented with inositol and yeast extract produced maximum acid phosphatase at in vitro conditions. Ferric chloride produced considerably higher acid phosphatase among different trace elements tested. The present study demonstrates various factors affecting acid phosphatase production, an important feature for selecting ectomycorrhizal mushrooms for field inoculations.[...] Read More.
Keywords: Dendrocalamus, Cantharellus, acid phosphatase, ectomycorrhizal mushrooms.
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文章
Full Length Research Paper
Abstract: Soft rot occurred severely in potato tubers stored in traditional and ameliored storehouses at Sikasso, Mali. 17 infective bacterial isolates were isolated from potato rot tissues (Solanum tuberosum L var. Odessa). Out of all, the isolate Od23 was found pathogenic and was characterized as rod-shaped, Gram positive, endo-spore formers and yellow pigment producers. This isolate which was found to be the principal organism responsible for potato rot in storage in Sikasso, grew at a temperature range from 5 to 45°C, with optimum temperature of 30 - 35°C. However, it showed strong pathogenicity to potato tubers at 30°C at 3 days. Furthermore, the 16S DNA analysis confirmed that the obtained isolate was Bacillus pumilus. All Potato varieties cultivated in Mali responded to infection with B. pumilus. Potato var. Sahel was the most susceptible, while Pamina appeared the most resistant potato variety from Mali. According to literature review, this is the first report on the occurrence of B. pumilus as a causal agent of potato soft rot in storage in the region of Sikasso, Mali.[...] Read More.
Keywords: Potato, storage, Bacillus pumilus, soft rot, Mali.
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文章
Moïse Nola*, Ernest Djarmaila, Norbert Kemka, Serge H. Zébazé Togouet, Nour-Eddine Chihib, François Krier, Pierre Servais, Jean-Pierre Hornez and Thomas Njiné
Abstract: The study carried out aimed at assessing the impact of groundwater storage temperature at household conditions on the temporal evolution of electrical conductivity and the future of heterotrophic aerobe bacteria (HAB). The storage duration was 7 days and the considered temperatures were 3, 10, 18 and 25°C. The electrical conductivity during storage reached 829 µS/cm at 3 - 18°C and 850 µS/cm at 25°C. The maximum HAB abundance was 9 x 103 cfu/ml at 3°C, 41 x 103 cfu/ml at 10°C, 44 x 103 cfu/ml at 18°C and 93 x 103 cfu/ml at 25°C. At the 3rd and 7th days storage at 3°C, changes in bacterial abundances values were significantly in the same direction as those of electrical conductivity (P < 0.05). The highest cell apparent growth rate at the 3rd day storage was 0.249 d-1 at 3°C, 0.559 d-1 at 10°C, 0.924 d-1 at 18°C and 1.233 d-1 at 25°C. However, at the 7th day storage, it was 0.362 d-1 at 3°C, 0.497 d-1 at 10°C, 0.690 d-1 at 18°C and 0.672 d-1 at 25°C. At the 3rd day storage, a decrease in cell abundance was noted in 90% of samples at 3°C and the cell apparent inhibitory rate varied from 0.012 to 0.989 d-1. The storage of groundwater in households’ conditions for a long period would alter its bacteriological quality.[...] Read More.
Keywords: Bacterial abundance’s evolution, electrical conductivity, groundwater, storage temperature, duration.
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Bin Li*, Ting Su, Rongrong Yu, Zhongyun Tao, Zhiyi Wu, Soad A. E. Algam, Guanlin Xie, Yanli Wang and Guochang Sun
Abstract: The inhibitory activities of seven Paenibacillus polymyxa strains and nine Paenibacillus macerans strains against Ralstonia solanacearum strains were examined. Result from this study indicated that the growth of all R. solanacearum strains except strain E406 were inhibited by P. macerans MB02-992 and P. polymyxa MB02-1007, while the other fourteen Paenibacillus strains had no in vitro inhibitory effect against R. solanacearum strains. In addition, suspensions of the two antagonistic bacteria showed antibacterial activities against R. solanacearum under different treatments and reduced the disease incidence and severity of tomato bacterial wilt. Overall, this study clearly demonstrates that antagonistic substances may play an important role in biocontrol of the two antagonistic bacteria. However, antimicrobial activities of P. macerans and P. polymyxa depend on the Paenibacillus strains and the target pathogen. This is the first report about the antibacterial activities of Paenibacillus strains against R. solanacearum strains isolated from different host plants.[...] Read More.
Keywords: Paenibacillus, antagonistism, biovar, Ralstonia solanacearum, tomato wilt.
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文章
Fahriye Eksi*, Ahmet Erbagci, Sakip Erturhan, Iclal Balci, Mehmet Solakhan and Faruk Yagci
Abstract: In this study urine and prostatic secretion samples of patients with chronic prostatitis were investigated for various prostatitis pathogens and the correlation between clinical symptoms. A total of 60 patients with chronic prostatitis were examined, prostatic secretion and urine specimens were evaluated under direct microscopy after staining with Giemsa and Gram. The selective media were used to investigate the presence of bacterial pathogens, Mycoplasma hominis and Ureaplasma urealyticum. Based on the laboratory findings of patients, 11(18.3%) were found to have chronic bacterial prostatitis, 49 (81.7%) were found to have chronic pelvic pain syndrome. Organisms isolated in patients with chronic bacterial prostatitis included Escherichia coli in four cases, Staphylococcus aureus in two cases and Pseudomonas aeruginosa, Klebsiella pneumoniae, coagulase- negative staphylococci, Enterococcus faecalis and diphtheroids in one case each. Of the 49 patients with chronic pelvic pain syndrome, U. urealyticum was isolated in seven and M. hominis in one of the patients. Premature ejaculation was more frequently detected in the patient group with chronic bacterial prostatitis than the patient group with chronic pelvic pain syndrome. Analysis of etiology of chronic prostatitis in our patients showed that U.urealyticum and E. coli were common pathogens of chronic prostatitis. We concluded that chronic bacterial prostatitis may increase the risk of premature ejaculation.[...] Read More.
Keywords: Chronic prostatitis, etiology, Ureaplasm urealyticum, Mycoplasma hominis, premature ejaculation.
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文章
Ismail Turkoglu, Semra Turkoglu*, Sait Celik and Mustafa Kahyaoglu
Abstract: This study was undertaken to examine in vitro antioxidant, radical scavenging and antimicrobial activities of extracts of Achillea schischkinii Sosn. and Achillea teretifolia Waldst. and Kitt (Asteraceae). The plant materials were extracted in methanol, water and chloroform using rotary evaporator apparatus. The extracts were screened for antioxidant activity using the ABTS radical scavenging capacity, DPPH radical scavenging capacity, superoxide anion radical scavenging, hydrogen peroxide scavenging and metal chelating activities and compared to standard antioxidants. The results obtained in this study indicate that A. schischkinii and A. teretifolia are potential sources of natural antioxidants, antimicrobial activity screening was performed by the disc diffusion method against 6 bacteria strains and 2 yeast species. A. teretifolia displayed strong inhibitory effects against Pseudomonas aeruginosa. The A. teretifolia extract also showed antimicrobial activity against Saccharomyces cerevisae. I contrast, the A. schischkinii extract showed no antimicrobial activity against gram-positive and gram-negative bacteria nor against yeast.[...] Read More.
Keywords: Achillea schischkinii, Achillea teretifolia, antioxidant, antimicrobial.
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文章
Ledong Sun, Caimei Zhong, Mingyu Chen, Bing Wang, Xueyan Liu, Xiufen Zheng, Fengjiao He, Zaigao Zhou and Kang Zeng*
Abstract: To establish a rapid, sensitive and specific detection method for deep fungal infection, we detected the deep infection of Candida albicans and Aspergillus flavus in patients with systemic lupus erythematosus by using multiplex fluorescent real-time PCR (MFRT-PCR). Firstly, the positive rate, sensitivity and specificity of multiplex fluorescent quantitative PCR for detecting the fungal infection was tested in 20 specimens from systemic lupus erythematosus patients with C. albicans and A. flavus infections. Then, 20 specimens from SLE patients with suspected deep fungal infections and 20 other microorganism infections were detected with MFRT-PCR. Results showed the positivity rate and specificity of both 100% for detecting the deep C. albicans and A. flavus infection. Its detecting sensitivity for deep fungal infection was 75%, which was significantly higher than that of fugal culture method (40%) (P < 0.05). MFRT-PCR is a better method for detecting deep C. albicans and A. flavus infections, with higher sensitivity and specificity than fungal culture.[...] Read More.
Keywords: Systemic lupus erythematosus, deep fungal infection, multiplex fluorescent quantitative PCR.
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文章
S. Hemalatha* and S. Shanthi
Abstract: Bacillus subtilis was isolated from milk samples. Antibiotic resistance and the antimicrobial activity of B. subtilis were studied. All the four isolates of B. subtilis were sensitive to antibiotics such as streptomycin (25 g/ml), ampicillin (10 g/ml), penicillin (10 g/ml), erythromycin (15 g/ml), amoxycillin (10 g/ml).But they were resistant to bacitracin (10 g/ml). B. subtilis shown antibacterial activity against the selected human pathogens such as Salmonella spp, Streptococcus spp, Klebsiella spp and E. coli. The antimicrobial substance from B. subtilis extracted with organic solvent such as ethyl actetate have also shown antibacterial activity against the human pathogens. The proteineceaous nature of the B. subtilis exerted antimicrobial activity. The amount of protein varied between 0.05 - 0.55 mg/ml and the protein was qualitatively analyzed by SDS-PAGE. The entire samples have shown peptide < 62 kDa.[...] Read More.
Keywords: Bacillus subtilis, antibiotics, Salmonella spp, human pathogens, ethyl acetate, SDS PAGE.
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文章
M.  Khanmohammadi*, E. Fallah, S. Rahbari, I. Sohrabi, M. Farshchian, F. Hamzavi and A. Mohammadpour Asl
Abstract: Visceral leishmaniasis (VL) is one of the most important endemic parasitic diseases in different parts of Iran. It is also a health problem in some tropical and subtropical countries. The purpose of the present study was to determine the seroprevalence of canine VL (CVL) in owned dogs of Sarab and to identify the isolated Leishmania species. Sera samples were taken from 384 dogs from 30 random villages around Sarab and tested by enzyme-linked immunosorbent assay (ELISA) . A total of thirty five dogs were seropositive. The seroprevalence rate (SPR) of CVL was 9.1% (95 % CI; 6.6 - 12.4). Out of 361 (94%) asymptomatic dogs, 31 (8.6%) were seropositive, and out of 23 (6%) symptomatic dogs, 4 (17.4%) were seropositive. 306 (79.7%) dogs were male and 78 (20.3%) were female. 28 (9.2%) male dogs and 7 (9%) female dogs were seropositive. There was no statistically significant relation between sex and seroprevalence (p = 0.962). The largest age group was 2 - 4 years, with 170 (44.3%) dogs out of which only 18 dogs (10.5%) were seropositive. Agreement between the ELISA test and clinical signs was 86.7%. A significant difference in the presence of antibodies against Leishmania was seen between symptomatic and asymptomatic dogs (p = 0.015) . ELISA was efficiently sensitive in the identification of infected dogs and seems to be an appropriate tool in rapid diagnostic screenings. ELISA is highly recommended to be applied as a standard test for routine CVL diagnosis.[...] Read More.
Keywords: Dogs, enzyme-linked immunosorbent assay (ELISA), epidemiology, Iran, Visceral leishmaniasis, prevalence, serology.
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