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文章
Keivan Beheshti Maal, Majid Bouzari* and Farahnaz Arbabzadeh Zavareh
Abstract: The aim of this research was to detect oral Streprtococci bacteriophages from Persian Gulf. Dental plaque samples were collected using sterile explorer and cultured in brain heart infusion (BHI) Broth. The oral Streptococci were isolated in culture media. The Persian Gulf water sample was gathered using a sterile bottle from the depth of 50 cm under the inframarine surface at Boushehr Port, Boushehr state, Iran. The Persian Gulf water was centrifuged and its supernatant was filtered through a 0.45 micrometers membrane filter and with a sterile Millipore filtration system. The filtrates were added to activate oral Streptococci at their logarithmic phase and cultured in (BHI) Agar using overlay method. Bacteriophage plaque forming assay in (BHI) Agar and clearance of (BHI) Broth suggested the presence of specific bacteriophages in sample. Transmission electron microscopy revealed that the capsid of the isolated bacteriophage was hexagonal (diameter: ~ 83.33 nm) most probably related to Cystoviridae family. This is the first report of isolation and identification of oral Streptococci bacteriophages from Persian Gulf located in South of Iran. The applications of these lytic phages as a potential for phage therapy of dental plaque could be considered as the significance and impact of the present study.[...] Read More.
Keywords: Persian Gulf, Streptococcus salivarius, bacteriophages, phage therapy, dental plaque, pharmaceutical and medical biotechnology.
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文章
N.Murugalatha*, A. Mohankumar, A. Sankaravadivoo and C. Rajesh
Abstract: Colour removal of industrial effluent has been a major concern in waste water treatment, especially for the waste water that originates from textile and dye stuff plant with a continuous discharge of great quantity of remaining dyes to the environment. The efficient treatment of the effluent is an eco- friendly method for the treatment of textile effluent. Bacillus species was isolated from the processed food-pickles and were characterized by means of biochemical reactions. Spore formers, non spore formers and their supernatant were used for the treatment. Effluent collected from the textile was diluted to 10, 50 and 90%, were subjected to biological treatment. The level of degradation rate was increased to 87.7 to 93.3% on dilution at 50 and 90% respectively when spore formers were used. The culture supernatant was able to degrade the dye at a rate of 34% with crude effluent and 98 with 90% of diluted effluent. Probably the metabolites produced by the organism were involved in the dye degradation process.[...] Read More.
Keywords: Textile effluent, Bacillus species, declorization, aerobic degradation.
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文章
T. Shafighi, G. Abdollahpour*, T. Zahraei Salehi and H. Tadjbakhsh
Abstract: A serological and bacteriological study was performed in Guilan industrial slaughter house, in Rasht, North of Iran in 2009. To investigate the seroprevalence of leptospirosis in slaughtered cattle in Guilan, 59 and 39 random serum samples were collected from cows and bulls, respectively. None of the cattles was vaccinated against leptospirosis. Also urine samples were collected from all of the blood-sampled cattle and cultured. All serum samples were serologically tested by microscopic agglutination test (MAT), as a standard method for serological diagnosis of leptospirosis. The serum samples were tested for antibodies against eight live antigens of Leptospira interrogans serovars: Australis, Autumnalis, Canicola, Grippotyphosa, Hardjo, Icterohaemorrhagiae, Pomona and Sejroe. The lowest dilution that each serum was considered positive was 1:100. The results of this study showed that 37 (37.8%) animals had a positive reaction against one or more serovars. The most prevalent Leptospira serovars was Pomona (49.0%). One leptospiral organism was isolated from 98 urine samples of cows and bulls. The results of this study indicates that leptospiral infection is magnified in cattle in Rasht, and cattle have a major role in maintaining Pomona serovar; indeed they are a potential zoonotic risk to slaughter house workers, meat inspectors, milkers and farmers.[...] Read More.
Keywords: Iran, Rasht, serology, bacteriology, cattle, leptospirosis.
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Uaboi-Egbenni, P. O., Bessong, P. O., Samie, A. and Obi, C. L.
Abstract: A total of 300 freshly voided sheep faeces were collected and screened for the presence of Campylobacter spp. using standard microbiological techniques. The samples were obtained randomly from 3 farm settlements in the Venda Region, South Africa in 2008 and 2009. The recovery rate was 30.0% (90 of 300) for all faeces. Of these, 65 (72.2%) were from diarrheic and 25 (27.8%) were from non-diarrheic faeces. Out of the 90 Campylobacter spp. isolated, 41(45.6%) were Campylobacter jejuni and 49 (54.4%) were C. coli. Sixty-three (70%) of the isolates were -haemolytic, while 17 (18.9%) were - haemolytic and 10 (11.1%) were non-haemolytic on 5% sheep red blood cells. The antibiotic resistance patterns of the 90 Campylobacter isolates were examined by the disc diffusion method. All Campylobacter isolates from the farms were resistant to at least one of the 12 antibiotics tested. The prevalence rate of C. coli resistance to ciprofloxacin was 20.4% compared with C. jejuni, 17.1%. Similar rates were noted for tetracycline for the two species. C. jejuni showed a higher rate of resistance to erythromycin (22.0%) compared with Campylobacter coli (10.2%). Significantly higher frequency of kanamycin resistance was recorded for C. jejuni compared to C. coli (p < 0.005). However, for ciprofloxacin, tetracycline, erythromycin, imipenem, gentamycin and ampicillin comparable resistant profiles were recorded for C. jejuni and C. coli isolates from the farms. The high prevalence of Campylobacter spp. in sheep is of public significance in the Venda Region. The observed multi-drug resistance and especially resistance to macrolides and fluoroquinolones in this study pose a threat of transfer of antibiotic resistance to human pathogens because of the close contact between sheep and humans.[...] Read More.
Keywords: Antibiotics, Campylobacter, haemolytic, macrolides, pathogens, resistance.
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F. Mollaamin*, K. Shahani poor, T. Nejadsattari and M. Monajjemi,
Abstract: A cluster model for active site of oxidized azurin was presented and investigated the geometric structure and thermochemical parameters. Quantum-mechanical calculations were performed at the HF and B3lYP/6-31G levels of theory in the gas phase and eight solvents at four temperatures. Also, nuclear shielding parameters of the active site of oxidized azurin have been taken into account using GIAO and CSGT methods at the HF and B3LYP/6-31G levels of theory in the gas phase and in different solvents such as water, DMSO, nitromethane, methanol, ethanol, acetone, dichloroethane and dichloromethane. The results were revealed that the NMR chemical shielding parameters are strongly affected by inducing different solvent media. According to these theoretical results of energy values, some important relationships have been found between the dielectric constant and structural stability of active site of oxidized azurin. Thus, it can be drastically concluded that the dielectric permittivity of the solvent is a key factor that determines the chemical behavior of active site azurin in solution.[...] Read More.
Keywords: Azurin, IR, nuclear magnetic resonance, blue copper proteins, solvent effect.
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文章
M. Akarcay*, M. C. Miman, O. Miman, M. Kelles and N. Ekici
Abstract: The aim of this study was to compare allergic rhinitis clinics caused by two most common allergens: house dust mites (HDM) and pollens. Three hundred and fifty patients were evaluated. These patients were pure pollen or HDM allergic according to skin prick test (51 HDM+, 299 Pollen+). Mainly, HDM were allergens for perennial allergic rhinitis, while pollens were for seasonal allergic rhinitis (p < 0.01). Both groups were found with similar symptom frequency except palatal, ocular, throat itching and eye redness indicating mostly pollen allergy (p < 0.05). Seasonal exacerbations used for the differential diagnosis were found to be very significantly different. Spring and autumn were the seasons where pollen allergy symptom exacerbation was mainly seen (p < 0.01) . HDM allergy was uniquely found with symptom exacerbations in winter (p < 0.05). Rural area visit was found dominating triggering factor for pollen allergy (p < 0.05). The most common triggering factor was house dust exposure in HDM+ group (p < 0.05). HDM allergy being mostly mimicking pollen allergy in allergic rhinitis, however, differs from it with some clinical features. This could be detected with detailed history taken from the allergic rhinitis patients. While doing definitive diagnosis, prick test may be helpful with a clear patient history in patients hard to diagnose.[...] Read More.
Keywords: Mite, pollen, allergy, rhinitis.
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Sasimar Woraharn, Chaiyavat Chaiyasut*, Busabun Sirithunyalug and Jakkapan Sirithunyalug
Abstract: Two processes of enclosure of probiotic Lactobacillus plantarum CMU-FP002, probiotic granules and calcium alginate beads, were studied. Sodium alginate solution at 1.0, 1.5 and 2.0 % (w/v) was used as a binder. The results showed that 20 log cfu/ml initial concentration of cells could be entrapped by the granules and beads with 12 to 13 log cfu/g and 16 cfu/g, respectively. The physical properties of granules and beads revealed that the strength increased when sodium alginate concentration was increased. On the other hand, the dissolution decreased. Probiotic granules completely released the cells within 60 min after being suspended in stimulate gastric fluid (SGF) pH 1.8 and had 2 to 3 log survival cells per gram. Calcium alginate beads, which were formulated from 1.0 and 1.5% (w/v) sodium alginate solution, gradually released bacterial cells and were completely released in SGF within 120 min. The beads formulated from 2.0 %(w/v) sodium alginate solution could not completely release the probiotics. The beads contained more survival cells than granules. Furthermore, the beads formulated from 1.5% (w/v) sodium alginate solution had the highest survival cells (9.30 log cfu/g). Probiotic cells in calcium alginate beads were still high (11 log cfu/g), although they were stored at 4˚C for 5 days alternating with room temperature for 5 days, for a total of 2 month. Further application in broilers will be studied.[...] Read More.
Keywords: Probiotic, survival enhancement, granulation, encapsulation, Lactobacillus plantarum.
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文章
Yavar Sharafi*, Jafar Hajilou, Seyyed Abol-Gasem Mohammadi and Mohammad Reza Dadpour
Abstract: Most of the Iranian almond cultivars are self-incompatible followed by pollination, fertilization, fruit set and lower yield problems. Therefore, selecting suitable cross-compatible cultivars for orchard establishment is necessary especially by new cultivars/genotypes obtained from breeding programs. In this study fruit set and pollen tube growth of ten late- bloom almond genotypes, obtained from a breeding program (D, E, F, I, G, L, K, O, P and Q) were investigated under filed and lab controlled pollination conditions. In order to study self-and cross-(in) compatibility they were pollinated by the pollens of overlap blooming-time genotypes in both conditions. Initial and final fruit set, fruit drop and some of the kernel traits were measured under filed condition. Measurements of pollen tubes at the style and in ovary were scored using fluorescence microscopy in lab, Results showed significant differences in some of the studied characters among crosses in both methods and Results confirmed each other in both methods. Fruit set percentage and pollen tube number in the ovary demonstrated that, all of the genotypes were self-incompatible but cross- (in) compatibility was not observed among them. In conclusion all of the genotypes could be used as a suitable polinizers for each others, regarding overlapping blooming-time of genotypes.[...] Read More.
Keywords: Almond, self-incompatibility, cross-incompatibility, fruit set, fluorescence microscopy.
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文章
Rohit Sharma*, Rakesh K. Baghel and Akhilesh K. Pandey
Abstract: Ectomycorrhizal mushroom Cantharellus tropicalis was grown in axenic culture to study the effect of pH, temperature, nitrogen, carbon, phosphorus and heavy metals/trace elements on production of acid phosphatase and mycelial growth. The results of present study showed optimum mycelial growth with pH 4 at 15 and 35 ± 2°C. The ectomycorrhizal mushroom mycelia utilized lactose and yeast extract as best carbon and nitrogen source for biomass production. Ferrous sulphate supported the maximum mycelial growth when different trace and heavy metal were used. Among phosphorus sources, di-Sodium hydrogen phosphate supported maximum growth. The acid phosphatase production did not follow a uniform pattern as inferred from observations in this study. Moreover, results showed that high biomass did not mean more acid phosphatase production. However, pH 5 at temperature 15 and 35 ± 2°C supported high enzyme production. Fries Das medium supplemented with inositol and yeast extract produced maximum acid phosphatase at in vitro conditions. Ferric chloride produced considerably higher acid phosphatase among different trace elements tested. The present study demonstrates various factors affecting acid phosphatase production, an important feature for selecting ectomycorrhizal mushrooms for field inoculations.[...] Read More.
Keywords: Dendrocalamus, Cantharellus, acid phosphatase, ectomycorrhizal mushrooms.
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Full Length Research Paper
Abstract: Soft rot occurred severely in potato tubers stored in traditional and ameliored storehouses at Sikasso, Mali. 17 infective bacterial isolates were isolated from potato rot tissues (Solanum tuberosum L var. Odessa). Out of all, the isolate Od23 was found pathogenic and was characterized as rod-shaped, Gram positive, endo-spore formers and yellow pigment producers. This isolate which was found to be the principal organism responsible for potato rot in storage in Sikasso, grew at a temperature range from 5 to 45°C, with optimum temperature of 30 - 35°C. However, it showed strong pathogenicity to potato tubers at 30°C at 3 days. Furthermore, the 16S DNA analysis confirmed that the obtained isolate was Bacillus pumilus. All Potato varieties cultivated in Mali responded to infection with B. pumilus. Potato var. Sahel was the most susceptible, while Pamina appeared the most resistant potato variety from Mali. According to literature review, this is the first report on the occurrence of B. pumilus as a causal agent of potato soft rot in storage in the region of Sikasso, Mali.[...] Read More.
Keywords: Potato, storage, Bacillus pumilus, soft rot, Mali.
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