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Direct ex vivo effects of herbal extracts on serum bilirubin in neonatal blood samples
Abstract: Herbal drug therapy is a common practice adopted in traditional and alternative medicine and has been used in the treatment of neonatal jaundice or hyperbilirubinemia from ancient times. This is done with the help of different herbal extracts and using different treatment methods. The knowledge of new possibilities and the fact that herbal drugs have been used in some communities for a long time, clarifies the importance of studying herbal drugs. Herbal drug therapies are used commonly which amplifies the need to evaluate the efficacy of these drugs. This study was performed to evaluate the effect of commonly used herbal drugs in neonatal jaundice, through in vitro techniques. In this study 0.5 cc (50 µg/ml) of different herbal extracts such as Chi corium intybus, Fumaria parviflora, Zizyphus jujuba and Alhagi pseudoalhgi, Purgative manna which were obtained through hydrochloric instillation and were added to 1 cc of neonatal serum with hyperbilirubinemia. Then the level of bilirubin was checked by diazo blank method. Among all of the herbal extracts, only Chi corium intybus demonstrated a lowering effect on indirect serum bilirubin. Although Chi corium intybus showed lower levels than the control group, but statistically there was no significant difference in direct bilirubin levels between these groups. Herbal drugs can decrease the bilirubin level through different mechanisms, such as increasing the activity of liver enzymes and decreasing the enterohepatic circulation. But the extract of the herb Chi corium intybus imposes its affect directly on bilirubin. Therefore further investigation is needed in order to separate the active agent in chi corium intybus.[...] Read More.
Keywords: Hyperbilirubinemia, Fumaria parviflora, Zizyphus jujuba, Chi corium intybus, Alhagi pseudoalhagi, Purgative manna.
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Orhue N. E. J*. and Nwanze E. A. C.
Abstract: A major feature of infection with trypanosomes is the development of anaemia. In this study, the effect of Scoparia dulcis ( Atiotiousha in Akoko-Edo) on Trypanosoma brucei induced anaemia was investigated in fifteen rabbits divided into three groups of n = 5 over a period of twenty eight days. Changes in Packed cell volume (PCV), Haemoglobin (Hb) concentration, Red blood cell count (RBC), Mean cell haemoglobin (MCH), Mean cell haemoglobin concentration, (MCHC) and Mean cell volume (MCV) were monitored over the period. The results obtained indicate that infection with T. brucei results in a significant decrease (p0.05) changes were observed in MCH, MCHC and MCV. However the severity of observed anaemia was significantly less pronounced (p[...] Read More.
Keywords: Scoparia dulcis, Trypanosoma brucei, Rabbits, PCV, Haemoglobin.
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文章
Hanaa H. Abd El-Baky
Abstract: This study investigated the antioxidant capacity and the levels of enhanced total carotenoids (TCAR), tocopherols (TOC) and phenolic (TPC) and protein (PC) contents in whole grains of wheat plants irrigated 10 and 20% (v/v) seawater (SW) in response to water extracts of microalgae Spirulina maxima (SME) and Chlorella ellipsoida (CEE) and exogenous plant growth enhancers of ascorbic acid (Vit. C) and benzyladinin (BA) treatments. Significant differences (P < 0.05) in amounts of TCAR (ranged 80 to 140 µg/g), TOC (ranged 50.4 to 115 µg/g), TPC (ranged 0.80 to 2.96 mg/g) and PC (ranged 9.34 to 13.79 %) in wheat grains among all treated plants were observed. The levels of their compounds increase related to irrigation-SW combined with algal treatments. The ethanolic extracts of grains of SW-stress plants treated with algal extracts exhibited high antioxidant capacity based on scavenging of DPPH and ABTS radicals than other samples. This activity remarked correlation with levels of antioxidant compounds present in these extracts. The electrophoretic profiles (SDS-PAGE fingerprint) of grains protein of treated samples exhibited similar pattern that in controls samples. It is concluded that the application of algal extracts to wheat plants irrigated SW lead to increase antioxidative components and protein content; hence consumption of these whole grains may render beneficial health effects.[...] Read More.
Keywords: Microalgae, antioxidant activity, phenolics, proteins, seawater.
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Ferdinandi Patrick, Godliving Mtui*, Anthony Manoni Mshandete, Gunnar Johansson and Amelia Kivaisi
Abstract: A lignolytic basidiomycete fungus, Funalia trogii (Berk.), was isolated from decayed wood in coastal Tanzania and cultivated in submerged culture. Initially screened crude enzyme filtrate showed complete rhemazol brilliant blue - R (RBBR) decolorization 2,2’-azino-bis (3-ethylbenzthiazoline)-6-sulfonate and guaiacol oxidation after 7 days of incubation. The fungal filtrate had maximum laccase activity of 593 U/ml after 15 days of incubation. A laccase was purified by anion exchange and size exclusion chromatography to good purity as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and isoelectric focusing (IEF). The isolated main component had a molecular weight of ca 58 kDa as determined by MS and an isoelectric point (pI) of 3.8. The optimal pH and temperature range for the purified laccase were 4.0 - 5.0 and 50 - 70 ºC, respectively, using 2, 6-dimethoxyphenol as a substrate.[...] Read More.
Keywords: Funalia trogii, laccase, anion exchange chromatography, size exclusion chromatography, electrophoresis, isoelectric focusing.
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Edmond Ahipo Dué*, Hervé César B. L. Zabri, Jean Parfait E.N. Kouadio and Lucien Patrice Kouamé
Abstract: Skin and digestive fat content (DFC) oils from Rhynchophorus palmarum L. larva (Curculionidae) were extracted and their physicochemical properties were characterized. Water content (0.41 %) of skin oil was higher than the amount of DFC (0.04 %). While, the lipid fraction of the skin (35.16%) was slightly lower than the DFC (49.05%). The fatty acid compositions of the both oils were determined. Results showed that the most abundant fatty acids in skin and DFC oils were palmitic and oleic acids. In both oils, oleic fatty acid showed the highest percentage of composition of 45.62 and 46.71% for skin and DFC, respectively with palmitic acid followed close by 39.87 and 40.44%, respectively. In this study, saturated fatty acids accounted for 45.06 and 44.97% of total fatty acids, for skin and DFC oils, respectively. Myristic, myristoleic, stearic and linoleic acids were also detected in the both oils. Physicochemical properties of skin and DFC oils respectively include: iodine index, 51.22 and 48.35; acid value, 4.72 and 2.21; saponification value, 189.22 and 198.26; unsaponifiable matter, 0.97 and 0.98; peroxide index, 6.90 and 0; oleic acidity, 7.76 and 0.568; vitamin A, 0 and 12.04 and refractive index, 1.45440 and 1.45424. Results suggested that Skin and DFC oils from R. palmarum L. larva could deserve further consideration and investigation as a potential new multi-purpose product for nutritional, industrial, cosmetic and pharmaceutical uses.[...] Read More.
Keywords: Fatty acids, digestive fat content, skin of larvae, oil, Rhynchophorus palmarum.
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Shree Prasad Thapa, Chun Keun Lim, Sam Kyu Kim, Jun Mo Cho, Jang Hyun Hur and Duck Hwan Park*
Abstract: A  rapid high-throughput, specific and sensitive (polymerase chain reaction) PCR-based assay coupled with DNA hybridization technique for the detection of deep bark canker (DBC) pathogen Brenneria rubrifaciens directly from mixed bacterial cells and in planta was developed. These result shows that the specific genetic markers have a powerful potential to detect B. rubrifaciens directly from crude samples at field conditions.[...] Read More.
Keywords: Brenneria rubrifaciens, deep bark canker, PCR (polymerase chain reaction), rubrifacine.
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Parichat Phumkhachorn and Pongsak Rattanachaikunsopon*
Abstract: A bacteriophage infecting Vibrio harveyi was isolated from shrimp pond water, it was designated PW2. It infected all strains of V. harveyi but not other bacteria used in this study. The phage adsorption rate increased rapidly in the first 15 min of infection to 80% and continued to increase to 90% within 30 min of infection. The stability of phage PW2 was dependent on temperature and pH. It was inactivated by heating at 90°C for 30 min and by treating at pH 2, 3, 11and 12. From its one step growth curve, latent and burst periods were 30 and 120 min, respectively with a burst size of about 78 plaque-forming unit (pfu) per infected center. As analyzed by transmission electron microscopy, phage PW2 had an icosahedral head (50 ± 3.8 nm in diameter) with a noncontractile tail of 136 ± 6.2 nm long and 11 ± 0.5 nm wide and belongs to the Siphoviridae family. Six structural proteins (75, 60, 35, 30, 20 and 15 kDa) were detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis, its genome was found to be double stranded DNA with an approximate size of 46 kb.[...] Read More.
Keywords: Bacteriophage, shrimp, Vibrio harveyi.
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文章
G. I. Okwu, Premila N. Achar* and Santosh K. Sharma
Abstract: A total of 150 ready to use food thickeners were randomly collected from various markets in both urban and rural settings. Four of the samples tested Achi (Brachystegia eurycoma), Akpalata ( Afzelia africana), Ofor (Detarium microcarpum), and Ukpo (Mucuna flagellipes) were contaminated with fungal flora. The most common and prevalent fungi observed on incubated powdered form of food thickeners on media, were the Aspergillus group namely Aspergillus flavus, Aspergillus parasiticus and Aspergillus niger. Pure isolates of A. flavus and A. parasiticus from various food thickeners from open markets in Nigeria were screened for their potential to produce aflatoxin B1 (AFB 1) on agar media. Ultraviolet (UV) light, a standard procedure was used to differentiate the toxin from non-toxin form of Aspergillus species. Further, aflatoxin quantification was done using thin layer chromatography (TLC) followed by fluorometry. A. flavus was more prevalent than A. parasiticus in all samples. Toxin and non-toxin isolates were grouped as per bright greenish-yellow fluorescence (BGYF) presumptive test under UV light. The amounts of AFB1 from A. flavus isolates ranged between 0.94 to 3.83 µg/g of agar and all positive A. parasiticus ranged from 0.22 - 2.87 µg/g of agar. Analysis of food thickeners also revealed a high incidence and alarming levels of naturally produced aflatoxin. The levels of AFB1 ranged between 4.0 and 95 µg/g in various food thickeners tested. That the presence of aflatoxin in food thickeners poses a potential health threat to consumers in this part of Nigeria and elsewhere is discussed.[...] Read More.
Keywords: Ready-to-use food thickeners, Aspergillus sp., aflatoxin B1, thin layer chromatography, fluorometry.
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Hu Yushan, Luo Lei, Liu Weijia, and Chen Xiaoguang*
Abstract: This study aims to develop a rapid method for identifying pathogenic bacteria based on the sequence difference of pathogenic bacteria groEL gene. A pair of universal degenerate primers was designed to amplify the groEL genes of 34 major species of 16 genus’s of pathogenic bacteria. The conservation, variation and the interspecies phylogenetic relations of the groEL gene sequences were informatically analysed. Our results showed that the groEL gene fragments of the selected pathogenic bacteria could be amplified using the degenerate primer. The results of the sequence comparative study indicated that the groEL gene is characterized by conservation and variation, while the variation regions are distributed separately among the conserved regions, just like the mosaic. This conservation and variation coexistence of pathogenic bacteria groEL genes could provide a solid basis for the rapid identification of pathogenic bacteria and the development of a diagnostic microarray.[...] Read More.
Keywords: groEL, homology, phylogenesis.
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Liang Renjie*, Shi Shidi and Sun Changsen
Abstract: The objective of the present study was to investigate potential of Glycyrrhiza glabra polysaccharides to offer protection against acute liver injury in rats. Rats were administered a single oral dose carbon tetrachloride (CCl4, 640 mg/kg b.w., 1:1 in groundnut oil) and sacrificed 7 days of post-treatment. Hepatic damage was assessed by employing biochemical parameters. Our results demonstrated that treatment of rats with G. glabra polysaccharides significantly prevented the increased activities of and aspartate aminotransferase (AST), alanine aminotransferase (ALT), ALP and LDH in serum. G. glabra polysaccharides treatment also restored CCl4-induced altered caspase-3, TGF- 1 and TGF- 1 mRNA. Our findings provide evidences to demonstrate that G. glabra polysaccharides treatment significantly offsets CCl4-induced liver injury in rats.[...] Read More.
Keywords: Glycyrrhiza glabra polysaccharides, rat, TGF- 1 mRNA, aspartate aminotransferase (AST), carbon tetrachloride.
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