Advanced Journal of Microbiology Research

Abstract: Dockovdia cookarum infection was studied in relation to the abundance and size classes of Lanistes libycus from Omi Stream, Ago-Iwoye, Nigeria, between September 1997 and June 1998. 57.6% of the 262 L. libycus recorded were collected during periods of heavy rainfall. 17.2% (45) of the L. libycus examined had D. cookarum infection. The water mite prevalences were higher in rainy season than in dry season, and ranged between 3.0% and 44.4% monthly. L. libycus in 21-30 mm size class had the highest prevalence (73.3%)…

Abstract: Using informatics tools to compare important species is now feasible as structural genomics continue in importance and establishment of structure-function relationships become a common way of comparative analysis. Currently, many of the technical issues involved in sequencing complete genomes have been solved. The smallness in size of chloroplast genomes facilitates being used for the discovery of disease resistance genes, introgression of important traits in transgenic plants, quantitative trait analysis and phylo…

Abstract: Inter Simple Sequence Repeat (ISSR) markers involve polymerase chain reaction (PCR) amplification of DNA using a single primer composed of a microsatellite sequence. ISSR technology rapidly reveals high polymorphic fingerprints and determines genetic diversity. We used genetic markers generated from selected ISSR primers to assess genetic diversity among a set of Tunisian date palm varieties. Seven primers were used to cluster 12 date palm varieties and 77 polymorphic markers were sufficient to identify all of the…

Abstract: Amplified fragment length polymorphism (AFLP) was used to assess genetic diversity among 100 selected bambara groundnut [Vigna subterranea (L.) Verdc] landraces from a diverse geographic area of Tanzania. Eleven informative AFLP primer combinations generated a total of 49 scorable polymorphic amplification fragments across the bambara groundnut accessions. Genetic distances between all accessions based on Jaccard’s variability index ranged from 0.1 to 0.68, with a total average of 0.3. The results showed that bamba…

Abstract: Transgenic Hibiscus sabdariffa plants have been produced by a tissue culture independent method using Agrobacterium tumefasciens transformation procedure. Embryo axes of mature seeds with one cotyledon excised were infected by immersion in a suspension of Agrobacterium LBA 4404 strain culture that carries pBal plasmid with β-glucuronidase p35SGUSINT and plant selectable marker Neomycin Phospho-Transferase gene (nptII). Following a 24 h co-cultivation with Agrobacterium strain and decontamination with cefotaxime, em…

Abstract: Wastewaters from olive mills and pulp and paper mill industries in Jordan have been characterized and treated using laboratory scale anaerobic and aerobic sequencing batch reactors, respectively. Nutrient requirements for these two industrial wastewaters were found to be less than what is usually reported in the literature for C:N:P ratio of 100:5:1 for aerobic treatment and 250:5:1 for anaerobic treatment. This was ascribed to the low biomass observed yield coefficients and relatively low removal efficiencies in t…

Abstract: Foliar parameters of Ficus in Nigeria were subjected to quantitative analysis. The morphometric analysis is based on ten quantitative parameters of the leaves of species. Principal compound analysis produced six groups whose characters are described. Highly significant positive correlation exists between leaf length and leaf width, leaf length and lamina length, leaf length and petiole length, lamina length and lamina width. Negative correlation was observed between leaf width and leaf length/width ratio, petiole l…

Abstract: The potential of a tissue culture medium gelled with cassava flour to support shoot proliferation of stem nodal sections of Uapaca kirkiana and Faidherbia albida was studied. A two factorial experiment was conducted with the first factor as type of gelling agent (80 g/L cassava flour; 80 g/L cassava flour mixed with 3.5 g/L agar; and 7 g/L agar) while the second factor was two plant species. Explants were obtained from 8-months old U. kirkiana and F. albida in vitro raised seedlings. Data included number of shoots…

Abstract: Effects of using carrot juice in the in vitro growth of Daucus carota were investigated. Callus was the only form of growth obtained after five weeks in culture. The increase in fresh weight, dry weight and moisture content of the explants with increasing concentration of carrot juice in the medium was much lower when compared to either the cytokinin benzyladenine (BA) and/or the auxin picloram. Spectrophotometric and chromatographic analyses revealed the presence of both indole-3-acetic acid (IAA) and abscissic ac…

Abstract: A simple, rapid and efficient method for isolating genomic DNA from lychee (Litchi chinensis Sonn.) was developed. This modified CTAB protocol include the use of 2 M NaCl, PVP, 5% mercaptoethanol and 80% ethanol in the extraction as well as reducing the centrifugation times during the separation and precipitation of the DNA. Isolated genomic DNA showed high purity and high quantity following restriction digestion analysis.[...]

Abstract: An efficient and reproducible protocol is required to achieve high frequency transformation from transformed calli. We report here high frequency plant regeneration from mature seed derived embryogenic calli of two recalcitrant indica rice cultivars HKR-46 and HKR-126 after partial desiccation treatment. Embryogenic and nodular callus was initiated on MS basal medium supplemented with 2.5 mg l-1 2,4-D, 500 mg l-1 proline, 500 mg l-1 casein hydrolysate, 30 g l-1 sucrose and 2.5 g l-1 gelrite. Several media with diff…

Abstract: In recent times efforts are being made to improve the productivity of bambara groundnut. Studies were initiated (i) to characterise and evaluate landraces and to select superior ones for irradiation, (ii) to induce genetic variation through gamma irradiation and (iii) to use biotechnological approaches to shorten the generation cycle. The results of the study indicated that gamma irradiation induced higher genetic variation of up to four times within the varieties used in the study compared to the unirradiated cont…

Abstract: A systematic approach to process optimization for production of rifamycin B was applied to a strain of Amycolatopsis mediterranei. Examination of the growth revealed 6 different morphologically distinct colonies on Bennett’s agar medium. Rifamycin B production in shake flasks by the six different colony types ranged between 0.5 and 1.2 g/l. There was a clear correlation between the colony morphology and rifamycin B productivity. The highest yield of rifamycin B (1.03-1.2 g/l) was obtained by using the orange-red co…

Abstract: Amplification of gene expression of the most productive colony type of Amycolatopsis mediterranei strain N1 under stress of chloramphenicol, resulted in isolation of a variant NCH with productivity of 2.56 g/l compared to 1.15 g/l by the parent strain N1 (2.2 fold increase) . This amplified variant has a further advantage of reduced variation in colony morphology with predominance of the most productive colony type. Using variant NCH, modification of the fermentation medium F1 by the addition of 0.1% yeast extract…

Abstract: Follicular population and repartition in the ovarian cortex was investigated in the ovaries of the Oulmès-Zaers cattle breed and its crosses. A total of 30 ovaries were collected at slaughterhouse in Casablanca and Rabat from Oulmes-Zaers breed and its crosses (2 genotypes) of 3 age groups (5 years). The histological study of the ovary revealed that the ovarian cortex is composed of different areas (from the periphery to the medulla), the non stratified epithelium of the ovary, the tunica albuginea composed of two…