Advanced Journal of Microbiology Research

Abstract: Occurrence and distribution of sugar fungi was studied from soil and leaf litter in coastal sand dunes of Orissa for a period of two years covering three distinct seasons. Fungal succession of litter was also studied. Microbial isolation and soil analysis was performed using standard procedures. Maximum population density was observed in the rainy season followed by winter and lastly summer. Higher microbial populations were encountered in plantation soil than the barren sand. They corresponded to the fluctuation o…

Abstract: Echinophora Platyloba DC. is one of the four native species of this plant in Iran. The aim of this five-step study was to investigate the antimicrobial properties of this plant. Dried aerial parts of the plant were extracted by ethanol 70% in percolator. The antimicrobial activity of ethanolic extract was evaluated against dermatophytes, Candida (C) albicans and gram positive bacteria by agar dilution method and microbroth dilution assay. Finally, the synergistic effect of Amphotericin B plus 5% ethanolic extract a…

Abstract: Anaerobic fungal isolates Caecomyces sp. from the feces of elephant and Orpinomyces sp. from buffalo rumen were co- cultured in-vitro with rumen bacterial and protozoal fractions collected from buffalo to observe the possible fate of these fungi in the rumen, if inoculated as microbial-feed supplements. When co-cultured together or separately with rumen bacteria and protozoa, Caecomyces sp. was adversely affected. However, bacterial and protozoal counts were higher, compared to the counts when grown alone. Similar…

Abstract: Maize (Zea mays L) belongs to the family Poaceae and it is mostly grown as food for man and feed for animal. A two-season experiment was conducted in 2006 and 2007, respectively, to determine the effect of storage condition and tillage operation on some fungal diseases and yield of maize. Analysis of variance indicated that storage condition significantly affected plant height (cm) 5.69; 6.26 at 9 weeks after planting. Tillage operation was statistically significant on leaf spot and blight infection at 9 weeks afte…

Abstract: Tomato seeds collected from farmer’s were treated with garlic tablet at concentration 1:3 w/v, 1:4 w/v, 1:5 w/v and 1:6 w/v for controlling seedborne fungal diseases. Seed health status of treated seeds was evaluated following blotter incubation method. Three different fungal pathogens viz., Aspergillus spp., Fusarium spp. and Penicillium spp. were identified from seed samples by blotter incubation method. Garlic tablet at 1:3 w/v dose showed better performance in increasing seed germination and reducing prevalance…

Abstract: This study was aimed at isolating and identifying the fungi associated with stockfish contamination in Jos Metropolis. A total of 100 stockfish samples were randomly purchased from four markets namely, Terminus, Kwararafa, Katako and Gada biu in Jos town, Plateau State, Nigeria. The stockfish samples were assayed for fungal contamination and moisture content using standard procedures. All the stockfish samples were contaminated with fungi. Seven different fungi were found to be associated with the stockfish samples…

Abstract: The Tanzanian edible mushroom species Coprinus cinereus was grown on sisal waste substrates supplemented with chicken manure with the aim to evaluate the effects of the chicken manure supplement on the antimicrobial activity of the mushroom’s extracts. Crude ethyl acetate extracts were prepared from the mushroom’s fruiting bodies harvested at pre-capping, capping and post capping stages, and the extracts were tested for antimicrobial activity, using the agar well method. The antimicrobial activity was observed only…

Abstract: SacC1 is a novel restriction endonuclease from Saccharomyces cerevisiae that recognizes the palindromic sequence 5’CTCGAC3’ cleaving both DNA strands upstream and downstream of its recognition sequence and makes a staggered cut at the distance of five bases from the recognition sequence on the upper strand and at the seventh base on the complementary strand. It shares similar characteristics with Sac I from Streptomyces achromogenes as well as Sst1 from Streptomyces Stanford and Psp124B1 from Pseudomonas species. I…

Abstract: Epitope tagging of yeast proteins has become an efficient tool for biochemical analysis of protein of interest. The epitope-tagged proteins can be used for western blotting, immunoprecipitation and immunofluorescence experiments without the need to raise specific antibodies, thus saving considerable time and expense. We have constructed plasmid containing FLAG tag with kanMX6 module, which allows selection of G418-resistant cells in yeast. The same set of primers that amplify module constructed by Bahler et al. (19…

Abstract: The paper deals with interactions of some microfungal strains isolated from rhizosphere soils from three different sites with ectomycorrhizal fungus Cantharellus tropicalis mycelium grown in vitro on agar plates. The rhizospheric fungi were isolated from 3 different sites of bamboo forest and grown against Cantharellus. The cross inoculation method showed that C. tropicalis was highly active against some fungi, thus resulting in different types and strength of interactions. Overgrowth was the most common interactio…

Abstract: In Iraq, there is attempts to transfer the various industrial carbon waste to veterinary proteins depend on microorganisms by using of chemical process. Five different co-culture combinations (1:1 ratio, 1 × 106 conidia)of Aspergillus niger and Trichoderma viride, mixing of A. niger and T. viride, in 24 and 48 h old monocultures of Aspergillus similar mixing of A. in 24 and 48 h old monoculture of Trichoderma and the monocultures of both were evaluated for their potential performance of cellulases production. The s…

Abstract: A specific protein inhibitor for the restriction enzyme (SacC1) has been purified from Saccharomyces cerevisiae approximately 21,000 fold and its inhibitory properties have been characterized. The isoelectric points (pI) of SacCI and its inhibitor are 9.0 and 5.22, respectively. The molecular weight of SacC1, the inhibitor and SacC1-inhibitor complex were estimated by gel filtration on a Sephadex G-100 column to be 64,000, 32,000 and 85,000, respectively. The inhibitor protein inhibits SacC1 catalytic activities ef…

Abstract: Antagonism between the fungal symbionts of bark beetles may represent a biologically significant interaction when multiple beetle species co-occur in a host tree. Since high density bark beetle populations rapidly and dramatically shift forest characteristics, patterns of competition between the obligate fungal associates of sympatric bark beetle species may have broad ecological effects. Primary and competitive resource acquisition between allopatric and sympatric isolates of mutualist fungi associated with the ba…

Abstract: Five fungi (Aspergillus niger, Penicillium chrysogenum, Rhizopus oligosporus, Rhizopus nigricans and Trichoderma longibrachitum) were used in the fermentation of Jatropha curcas kernel cake for a 7 days period in a completely randomized design model. The results revealed significant increase in the crude protein content of all the fungi treated samples with Aspergillus niger and T. longbrachitum treated cake recorded higher value compared to other treated samples. There was no significant difference in the crude fi…