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Hossein Hassanpour Darvishi*, Mohammad Manshouri, Hossein Sedghi and Sayed Habib Mousavi Jahromi
Abstract: In this experiment, we have used the soil profile as a biological filter that can to absorb pollution and microbes in the domestic wastewater at Iran during 2009. In this experiment, we had 15 lysimeters, that were planted canola (Brassica napus L.) in 1 to 5 lysimeters and were irrigated by domestic wastewater with BOD5 about 150 mg/lit and primary drainage water were accumulated. In the 6 to 9 lysimeters was planted alfalfa (Medicago sativa L.) and were irrigated by primary drainage water and then, were accumulated secondary drainage water. We have irrigation 10, 11 and 12 lysimeters by secondary drainage water that was planted inside the basil (Ocimum basilicum L.). In order to compare plants characteristics, in 13, 14 and 15 lysimeters were planted canola, alfalfa and basil respectively and were irrigated by agronomical water. The results showed that the soil could reduce BOD5 and COD from 150 and 232 mg/lit to 11 and 18 mg/lit respectively in secondary drainage water. The essential oil yield of basil 17% increased under irrigation by secondary drainage water into irrigation by agronomical water. Therefore, the use of secondary drainage water can be increase the quantity and quality yields in plants and the other hand, protects sweet water resources.[...] Read More.
Keywords: Domestic wastewater, agronomical water, essential oil yield, basil.
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Chen-Xiaoxi and Yue Jun*
Abstract: The antibiotic produced by a newly isolated Bacillus subtilis has potent anti-tubercular activity against sixteen strains of Mycobacterium tuberculosis, most of which belong to MDR strains. MICs for the sixteen strains were evaluated. A series of spectrum analysis and element analysis determined that the antibiotic’s molecular formula was C27H46O2.[...] Read More.
Keywords: Bacillus subtilis, antibiotic, MIC, serum, anti-tubercular.
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Lu Hui, Li He , Lu Huan, Li XiaoLan* and Zhou AiGuo
Abstract: In this study we assessed the chemical composition, antioxidant and antibacterial activities of Lavender essential oil. The antioxidant and antibacterial capacity of test sample was assayed by a linoleic acid system and conventional method of bacterial growth inhibition. The results demonstrated that the essential oil consisted of 1,5-Dimethyl-1-vinyl-4-hexenyl but yrate as the most abundant component (43.73%), followed by 1,3,7-Octatriene, 3,7-dimethyl- (25.10%), Eucalyptol (7.32%), and Camphor (3.79%). Lavender essential oil display the stronger antioxidant activity against lipid peroxidation in a linoleic acid model system and good antibacterial activity against four rhinitis-related bacteria including staphylococcus aureus, Micrococcus ascoformans, Proteus vulgaris and Escherichia coli.[...] Read More.
Keywords: Antibacterial, Lavender; antioxidant, essential oil, extraction, rhinitis.
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M. Sunitha*, P. Ellaiah and R. Bhavani Devi
Abstract: The medium components influencing the enzyme production were screened using Plackett-Burman design. A total of 67 nutrients comprising of fifteen each of carbon, nitrogen, mineral/salt and eleven each of inorganic nitrogen sources and amino acid sources were screened for the production of L-asparaginase by SmF. This design involves screening of up to ‘n-1’ variables in just ‘n’ number of experiments. Regression coefficients and t-values were calculated by subjecting the experimental data to statistical analysis. Among the 67 nutrients based on their performance in terms of product yield and cost, tapioca starch, L-asparagine, ammonium oxalate, gelatin and CaCO3 were identified as most effective and therefore selected for inclusion in surface methodology studies.[...] Read More.
Keywords: L-asparaginase, nutritional factors, submerged fermentation, Bacillus cereus MNTG-7.
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Z.  A. Zakaria*, A. S. Sufian, K. Ramasamy, N. Ahmat, M. R. Sulaiman, A. K. Arifah, A. Zuraini and M. N. Somchit 
Abstract: The aim of the present study is to determine the in vitro antimicrobial activity of various extracts, partitions and fractions of Muntingia calabura (Elaeocarpaceae) leaves against a selected panel of microorganisms. The leaves of M. calabura were soaked separately in the aqueous, chloroform and methanol solvent systems in the ratio of 1:20 (w/v) for 72 h and these procedures were repeated three times. Antimicrobial testing was carried out using the micro -broth dilution method. The microbes targeted were Staphylococcus aureus 25923, S. aureus 33591 (a multi-drugs resistant S. aureus (MRSA) isolate), Escherichia coli 35218, Pseudomonas aeruginosa ATCC 27853, Candida albicans 10231 and Microsporum canis ATCC 36299. The methanol extract inhibited MSSA (MIC = 1250 µg/ml; MBC = 1250 µg/ml) and MRSA (MIC = 1250 µg/ml; MBC = 1250 µg /ml) and considered the most effective extract and was further partitioned sequentially using the aqueous, petroleum ether and ethyl acetate. The ethyl acetate partition exhibited effective antibacterial activities with the MIC/MBC value of 156 and 313 µg/ml against S. aureus 25923 and S. aureus 33591, respectively. The ethyl acetate partition underwent fractionation process and yielded 15 fractions (A1-A15) of which only fractions A9 to A15 effectively inhibited the growth of S. aureus 25923 and S. aureus 33591 with MIC/MBC values ranging from 78 to 2500 µg/ml.[...] Read More.
Keywords: Muntingia calabura, Elaeocarpaceae, antimicrobial activity, micro-broth dilution, ethyl acetate fraction.
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Omilabu A. Sunday*, A. Bankole Munir, O. Oyefolu Akeeb, A. Adesanya Bolanle and S. O. Badaru
Abstract: Studies on extracts and biologically active compounds isolated from medicinal plants have doubled in the last two decades. The leaves of Hibiscus sabdariffa (red and green leaved) and Celosia argentea were studied for their antiviral activities against Measles Virus (MV) as well as the effects of the extracts on Hep -2 cells. Ethanol extract of the leaves of each of these plants showed no toxicity to the Hep-2 cells at all concentrations used (5, 10 and 15 mg/ml). The pre-inoculative treatment of Hep-2 cells with plant extracts showed that C. argentea had no antiviral activities on MV at all concentrations (5, 10, 15 mg/ml) while H. sabdariffa had antiviral activities only at 10 and 15 mg/ml on MV. The post-inoculative treatment of Hep-2 cells with plant extracts showed that at 5, 10 and 15 mg/ml concentrations, H. sabdariffa had antiviral activities on MV while the antiviral activity of C. argentea could not be established at 10 and 15 mg/ml but did not show any antiviral activity at 5 mg/ml.[...] Read More.
Keywords: Hibiscus sabdariffa, Celosia argentea, antiviral, toxic, Hep-2 cell, herb, medicinal plant.
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文章
Chang Gao Wang, *, Ming Kai Chen and Tao Chen
Abstract: A novel microbial phospholipase C from Bacillus mycoides strain 970 was purified to apparent homogeneity by an improved purification process, in which DEAE-Cellulose adsorption was first used to remove bulk of dark brown impurities and then a combination of DEAE-Cellulose ion-exchange, Phenyl Sepharose hydrophobic interaction and Sephacryl S-100 gel filtration chromatography was used to separate the enzyme from remained contaminants. The enzyme appeared to be a single peptide of 75.1 kDa on SDS-PAGE. The optimum temperature and pH of the enzyme were 60°C and 7.0 - 7.5, respectively. The enzyme was stable at temperatures lower than 50°C and pH 5 - 9.5. This purified phospholipase C was characterized as a metallophospholipase C and Ca2+, Mg2+ , Zn2+, Ba2+ were essential for its PLC activity. The enzyme showed no lecithinase activity on egg yolk agar and also no hemolytic activity on blood agar.[...] Read More.
Keywords: Phospholipase C, purification, metalloenzyme, nonhemolytic activity, Bacillus mycoides.
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Jing Li, Shu Zhang, Guanghua Liu, Rui Zhao, Hui Xu, Dairong Qiao and Yi Cao*
Abstract: 12 strains of lipase - producing were isolated from 36 samples collected from oil - contaminated soil of machinery - repair plant, dining room, and vegetable market, respectively, of which strain (3 - 2) had the highest hydrolytic activity (17.1 U/ml). Immobilized cell of strain 3 - 2 onto sodium alginate and immobilized crude enzyme solution of strain (3 - 2) onto Sodium alginate were respectively used in two transesterification programs to transfer the Fatty acid methyl ester (FAME). Results showed that both immobilized crude enzyme solution and cell were of high transesterification efficiency for Transesterification Program I, respectively 32.72 and 26.84%. Cycle test done in this Program found that immobilized crude enzyme solution and cell still keep some transesterification efficiency after being recycled thrice at least. Meanwhile, hydrolytic activity of crude enzyme solution had some correlation with transesterification efficiency. However, hydrolytic activity of immobilized cell did not have a clear correlation with the latter. Strain (3 - 2) belonged to Sphingobacterium multivorum. Up to now, the transesterification research has not been reported.[...] Read More.
Keywords: Lipase, crude enzyme solution, bacterial cell, immobilized, transesterification.
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Özgür Güçlü*, Halil Bıyık and Aslı ahiner
Abstract: The aim of this study was to investigate the mycoflora of the loggerhead turtle, Caretta caretta, from its nests and eggshells at Fethiye Beach, Turkey. During the 2004 breeding season, following the complete emergence of the hatchlings, sand samples were collected from 15 nests and eggs from these nests were swabbed. Rose Bengal chloramphenicol agar was used for isolation, and then mycoflora were subcultured onto suitable media. Fungi were counted and identified at genus level. Ten genera were identified within the nests and eggshells. Aspergillus sp., Chrysosporium sp., Fusarium sp. and Penicillum sp. were identified both in nests and eggshells. Furthermore, while Absidia sp., Cylindrocarpon sp., Emericella sp. and Mucor sp. were only identified in the nests, Cladosporium sp. and Thielavia sp. were only identified in eggshells. In addition, there was a positive correlation between total number of isolated fungus and embryonic death in nests (r = 0.53, p < 0.05). Hatching success was negatively correlated with total number of isolated fungus (r = -0.54, p < 0.05) . Our study indicates a relation between hatching success and fungal flora of the nests. Presence of fungi may also be important for the researchers who digs nest to avoid infections or allergies due to these fungi.[...] Read More.
Keywords: Mycoflora, fungus, Caretta caretta, Fethiye beach.
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文章
Linhui Li*, Qingyu Yin, Xiaohong Liu and Hong Yang
Abstract: An  efficient  protocol  for  protoplast  isolation  and  regeneration  was  first  established  in  Coprinus Comatus. The highest yield of protoplasts was up to 8.9 × 106 cells/ml in digestion solution containing 2.0% lywallzyme and 0.6 M KCl after incubation of four-day-old mycelia at 30°C for 3 h; among which, about 1.4% protoplasts could be regenerated into mycelia after 4 - 6 days of incubation at 25°C in CYM medium with 0.6 M mannitol as osmotic stabilizer. The results are beneficial for breeding new cultivars by the methods such as protoplast fusion, mutagenesis as well as transformation. Moreover, the stepwise procedure for protoplast liberation and regeneration could be referred in other species.[...] Read More.
Keywords: Coprinus comatus, mycelia, lywallzyme, protoplast, liberation and regeneration.
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