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S. Rehman*, Tariq Mir, A. Kour, P. H. Qazi, P. Sultan and A. S. Shawl
Abstract: Nodulisporium sp. an endophytic fungus identified by 28s ribosomal gene sequencing isolated from a medicinal plant, Nothapodytes foetida was studied for its in vitro antimicrobial activity. Dual culture studies were carried out for antifungal activity where maximum antagonistic activity was against Alternaria alternata and Colletotrichum gleosporoides. For antibacterial studies, Gram positive and Gram negative human pathogens strains were used. The minimum inhibitory concentration (MIC) of ethyl acetate and methanol fractions of Nodulipsporium showed appreciable growth inhibition mainly active against disease causing Gram positive bacteria.[...] Read More.
Keywords: Nothapodytes foetida, endophytes, antagonism, antimicrobial, Nodulisporium, sp., pathogen, bacteria, minimum inhibitory concentration.
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Idise Okiemute Emmanuel* and Ofiyai Odoyo
Abstract: Wine was produced from pawpaw (Carica papaya) at a ratio of 1:4 for pawpaw must: water in fermentation vessels A to D. Fermentation vessel A pawpaw must contained natural yeast and water; B contained natural yeast from pawpaw and sugar solution; C contained natural yeast, activated baker’s yeast and sugar solution; and D (control) contained sugar solution and Baker’s yeast. Pawpaw wines produced had average values of 3.84, 29.6, 0.628, 0.9950, 0.464, 1.348, 6.66 and 0.54; 3.76, 29.6, 0.631, 1.0036, 0.623, 1.358, 6.89 and 0.37; 3.86, 29.8, 0.718, 0.9994, 0.419, 1.354, 6.32 and 0.78; and 3.33, 29.6, 0.659, 0.9974, 0.216, 1.351, 6.72 and 0.8 for pH, temperature (°C), optical density (at 560 nm), specific gravity, percentage titratable acidity, percentage alcohol (v/v), total aerobic count (log10cfu/ml) and retardation factor (Rf) (cm). Fermentation was carried out for 144 h, and it was observed that malo-lactic fermentation after 48 h was evident. Testing of the wine’s taste showed very little differences in the wines from Recipes A – C, while statistical analyses at 95% confidence level showed no significant differences. The wine from the control had similar taste and characteristics with natural palm wine. Pawpaw wine could thus be produced for immediate consumption, or preserved by refrigeration using Recipes A - C. More research is, however, required to determine the shelf stability of the pawpaw wine.[...] Read More.
Keywords: Pawpaw, flora, fermentation, sugar, wine, flavor, yeast.
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Valerie Gonsalves, Shweta Nayak and Sarita Nazareth*
Abstract: Halophilic mycobiota was isolated from Mandovi estuary and it was dominated by Aspergillus and Penicillium species. Cladosporium and Eurotium were found in lesser numbers while obligate halophiles were found only amongst the aspergilla and they were all identified as Aspergillus penicillioides. Some aspergilli and all the isolates of Penicillium, Cladosporium and Eurotium were facultative halophiles. There were significant differences in growth of each isolate at different salt concentrations. Most of the isolates were euryhaline, having a wide range of salt tolerance; a few were stenohaline, with a narrow range of halotolerance. The isolates were mainly moderate halophiles, with a very few slight halophiles. Isolation of obligate halophilic fungi from polyhaline environment of an estuary is hereby reported for the first time.[...] Read More.
Keywords: Aspergillus penicillioides, obligate halophiles, facultative halophiles.
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Sideney B. Onofre*, Cristiane R. Kasburg, Danusa de Freitas, Silvana Damin, Andréia Vilani, Jéssica A. Queiroz and Francini Y. Kagimura
Abstract: In this work the fungicidal action of three agrochemicals (Sphere®, Nativo® and Bendazol®) used in soybean for control of fungal diseases on the lineages CG-28 and CG-30 of Metarhizium anisopliae var. anisopliae was evaluated. It was found that the fungicides inhibited the vegetative growth of the lineages at the concentrations indicated for the field, thereby showing its antifungal effect.[...] Read More.
Keywords: Biocontrol, crops, pesticides.
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Aiping Zheng* and Yanran Wang
Abstract: The isolate Rhizoctonia solani AG-1 IB collected from the diseased leaves of soybean were identified by the method of internal transcribed spacer sequence analysis. In this study, we focus on the biological characteristics and infection process of AG-1IB. Morphological, nucleus, chromosome, and infection process were observed. Typical infectious structures as infection cushion and appressorium were observed during the infection process.[...] Read More.
Keywords: Rhizoctonia solani AG-1 IB, soybean, biological characteristics, infection process.
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Radha Pyati*, Lindsay L. Elvir, Erica C. Charles, Umawattee Seenath and Tom D. Wolkow
Abstract: The morphology of Schizosaccharomyces pombe can be rapidly monitored in asynchronous, G2-rich populations using imaging flow cytometry (IFC). Cell morphology was analyzed in terms of length and aspect ratio before and after exposure to several toxins. The toxins target the DNA (hydroxyurea and phleomycin) or cytoskeletal elements (thiabendazole, carbendazim and latrunculin A) and exert well-characterized effects on the morphology. Using IFC and yeast mutants, predictable morphological changes were detected accompanied with loss of gene products required during cellular responses to these toxins. IFC is a sensitive tool for accurate detection of subtle morphological changes in large, asynchronous S. pombe populations.[...] Read More.
Keywords: Imaging flow cytometry, fission yeast, Schizosaccharomyces pombe, cell cycle, morphology, genotoxin, cytoskeletal toxin.
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P. Senthilraja*, K. Kathiresan and K. Saravanakumar
Abstract: Yeasts are well known for bioethanol production. However, marine yeasts are less known for the activity. In the present context of increasing demand for energy and biofuel, the microbial synthesis of ethanol using cellulosic waste materials has gained recent importance. The present study deals with the identification of potential marine yeasts for ethanol production. Ten species of marine yeasts were cultured for 24, 48, 72, and 96 h for bioethanol production. Of the ten species, CANDIDA ALBICANS exhibited the maximum production of ethanol (47.3±3.1 g/L) within 96 h, when glucose was used as carbon source. The ethanol production by this species was found higher when the yeast cells were immobilized in sodium alginate compared to suspension culture. This experiment was also conducted with both immobilized yeast cells and non-immobilized cells. The experiment revealed that the marine yeast C. ALBICANS is efficient in bioethanol production, when it is immobilized.[...] Read More.
Keywords: Bioethanol, marine yeast species, monoclonal antibodies, thermotolerant.
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K. Mukesh Upadhyay*, A. K. Pandey and R. C. Rajak
Abstract: The present study was undertaken to assess the genetic diversity and phylogenetic relationship among the morphologically similar and ecologically variant isolates of Lactarius deliciosus using polymerase chain reaction (PCR) based techniques random amplification of polymorphic DNA (RAPD), rDNA analysis and isozyme patterns. Two major groups were defined amongst the total isolates by cluster analyses. Four bands of amylase isozymes viz; A232.4, A342.7, A461.5, A672.0 and two bands each of pectinases (Pe364.18, Pe261.11), cellulases (C460.14, C356.12), glutamate dehydrogenases (GLD126.00, GLD229.18) and acid phosphatases (AP110.00, AP214.18) were common in all the morphologically similar isolates. The RAPD profile revealed that a total of 12 polymorphic bands were obtained, among these a locus of 550 bp was diversified in all strains except M221 and M222. Few common bands which is present in zymogram (isozymes) and electrogram (RAPD) exhibited genetic similarities amongst ecologically variant isolates. Amplifications of the ITS region resulted a single PCR product of approximately 560 bp for all the isolates. When amplified fragments were digested with restriction enzymes, identical banding patterns were observed, which showed that no inter-generic or intra-specific variation among the isolates.[...] Read More.
Keywords: Ectomycorrhizal, Isozyme, random amplification of polymorphic DNA, restriction fragment length polymorphism, zymography.
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K. Girish,* and S. Shankara Bhat,
Abstract: 16 isolates of Phomopsis azadirachtae, causing die-back of neem were isolated from 16 different geographical regions of Tamil Nadu. Their growth response to physical (temperature and pH) and chemical (carbon and nitrogen sources) factors were investigated. The toxicity of culture filtrate of each isolate on neem seed germination was also studied. Several isolates exhibited maximum growth at 35°C. Maximum growth of all the isolates was observed at pH 6.0. Many isolates preferred starch and a few, sucrose, as carbon sources. Ammonium sulphate formed the good nitrogen source for most of the isolates. However, all the isolates displayed significant differences among themselves in their growth response to each treatment. The culture filtrate of each isolate inhibited the germination of neem seeds but exhibited varying degree of toxicity. The results obtained suggest the existence of physiological variability within P. azadirachtae.[...] Read More.
Keywords: Die-back of neem, carbon source, culture filtrate, isolates, nitrogen source, pH, Phomopsis azadirachtae, temperature, variation.
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Karim Dagno*, Julien Crovadore, François Lefort, Rachid Lahlali, LudivineLassois and M. Haïssam Jijakli
Abstract: Water hyacinth (Eichhornia crassipes) causes environmental, agricultural and health problems in Mali. This is particularly severe in the District of Bamako and the irrigation systems of the “Office du Niger” area. During two years survey for fungal pathogens of water hyacinth infested areas, isolate Mlb684 was collected from diseased plant. This fungal isolate was identified as a potential mycoherbicide for sustainable management for water hyacinth. The aim of this study was to characterize isolate Mlb684. The characterization was based on a morphological description and a DNA sequence analysis. Various genes amplified from isolate Mlb684 were compared to those existing in Genbank. These genes were 18S ribosomal rDNA gene, ITS rDNA gene, elongation factor-1 alpha (EF1a) gene, calmodulin and actin genes. DNA sequence comparisons and morphological description provided enough evidences to show that isolate Mlb684 belonged to the Alternaria genus and was distinct from any other known Alternaria species. Based on these evidences, the new fungal isolate was called “Alternaria jacinthicola Dagno & M.H. Jijakli”. A specimen culture has been deposited in the Gembloux Agro Bio Tech Plant Pathology unit fungal collection, with Mlb684 reference and in the Industrial Fungal and Yeast Collection (BCCM/MUCL, Belgium) under the accession number: MUCL 53159 and all DNA sequences were deposited in GenBank (NCBI).[...] Read More.
Keywords: ITS rDNA, 18S rDNA, actin, calmodulin, elongation factor, genetic characterization, Alternaria jacinthicola, Eichhornia crassipes, water hyacinth.
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