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Priscila Becker Siqueira*, Helena Maria André Bolini and Gabriela Alves Macedo
Abstract: Beer is a beverage obtained through alcoholic fermentation of malt wort, usually made of barley, which could be added of other cereals, such as corn, rice or wheat. Its alcoholic content is between 3 and 8%. It can be considered a good source of polyphenols derived both from malt and hop. Due to its antioxidant capacity and low alcoholic content, its able to improve plasma antioxidant activity, reducing the risk of cardiovascular diseases, without the negative effects of high doses of alcohol. Beer is an unstable product that is involved in a series of chemical, physical and sensorial transformations during its shelf life. This study evaluated the oxidative profile of two different types of Brazilian beer submitted to a forced aging process (6 days at 42°C) and natural aging (4 months at room temperature). The applied tests were: total polyphenol content, hydrogen-donating ability (DPPH), reducing power and copper reducing activity. Results showed no changes in total polyphenol content or antioxidant capacity during forced aging. Beers aged naturally showed a decrease in their polyphenol content and antioxidant capacity.[...] Read More.
Keywords: Beer, polyphenols, antioxidant capacity, aging, storage.
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文章
B. J. Akinyele* and C. O. Akinkunmi
Abstract: Fungi responsible for the spoilage of some berry, namely pawpaw (Carica papaya), sweet orange (Citrus sinensis), banana (Musa acuminata), garden egg (Solanum melongena), lemon (Citrus limoni), and tangelo (Citrus tangelo) with respect to their control using electromagnetic field were investigated. Fungi isolated include the genera of Aspergillus, Penicillium, Rhizopus, Articulospora, Gonatobotryum, Varicosporium, Trichoderma, Blastomyces, Fusarium, Pleurothecium and yeast, Saccharomyces. The fungal isolates were treated by exposure to electromagnetic field strength generated at voltage of 7, 10, and 13 V for periods of 0, 15, 30, 45, and 60 min, respectively. Growth of the fungal isolates was inhibited by the electromagnetic field with the inhibitory effect becoming more pronounced with increase in the field intensity and period of exposure. Apparently, healthy fruits were exposed to the highest electromagnetic field generated (13 V) for maximum time of exposure used (60 min). Proximate and mineral analyses of the treated and untreated fruits revealed that electromagnetic field wave has no negative or adverse effects on the nutrient components of the fruits. Therefore, electromagnetic field wave can be used in controlling spoilage fungi, thereby increasing the shelf life of fruits.[...] Read More.
Keywords: Berry, electromagnetic field, spoilage, fungi, voltage.
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文章
Bankole, P. O.*, and Adekunle, A. A.
Abstract: The biodiversity of mushrooms in Lagos State, Nigeria was studied using modern biotechnological method of DNA sequence analyses. Sixteen mushrooms were collected in Lagos State for 12 months. The mushrooms collected include: Agaricus campestris, Coprinus comatus, Daldinia concetrica, Ganoderma adspersum, Ganoderma applanatum, Ganoderma lucidum, Mycena haematopus, Mycena sp., Pleurotus ostreatus, Pleurotus tuber-regium, Polyporus sp., Polyporus squamosus, Polyporus sulphureus, Trametes versicolor, Xylaria polymorpha, and Xylaria sp. Subsequently, eight different pure mushroom mycelia were isolated using potato dextrose agar medium (PDA) from sterile tissues of A. campestris, C. comatus, G. lucidum, P. ostreatus, P. tuber-regium, P. sulphureus, X. polymorpha, and Xylaria sp. A mycelia spawn of each of the isolated mushrooms was made and kept in duplicates for DNA sequence analyses. The DNA sequence analyses of each of the eight mushroom mycelia pure isolates were carried out. No similarity was observed when a BLAST query of the mushroom DNA sequences was carried out to determine the similarity with the GenBank database previous submissions. The sequences were then submitted to the GenBank database for reference purpose. Comprehensive description given by the GenBank provides a detailed, reliable and accurate identification than visual characteristics and phenotypic properties of the mushrooms.[...] Read More.
Keywords: Biodiversity, mushroom, deoxyribonucleic acid (DNA) sequence, phylogenetic tree.
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文章
Shumin Yao*, Jing Huang and Jingli Tan
Abstract: Phytase is a new-style enzyme used in animal feed additive. It can increase phosphorus availability, decrease environmental phosphorus pollution and improve the performance of animals. Phytase gene was cloned by reverse transcription-polymerase chain reaction (RT-PCR) using first strand cDNA as template after reverse transcripting Aspergillus niger total RNA. The phyA gene was cloned into plasmid pYD1 which allows regulated expression, secretion and detection of expressed proteins on the surface of Saccharomyces cerevisiae cells using immunofluorescence. The construct was propagated in Escherichia coli DH5α and then was transformed into the yeast strain EBY100. After induction, the phytase activity was measured every 12 h. The results indicated that the activity of the fusion protein reached the highest level after being induced with 2.0% galactose for 48 h. This enzyme had pH optima (pH 7) and its optimum temperature was about 65°C.[...] Read More.
Keywords: Yeast surface display, Aspergillus niger, phytase.
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Suhaib A. Bandh*, Azra N. Kamili, Bashir A. Ganai, Samira Saleem, Bashir A. Lone and Humera Nissa
Abstract: Filamentous fungi comprehend a heterogeneous group of heterotrophic microorganisms that act as saprobes or parasites or, less frequently as symbionts living in association with other organisms. Water samples obtained seasonally from April 2010 to March 2011 at sixteen different sites of Dal Lake, Kashmir were serially diluted five folds followed by spread plate technique for the isolation of filamentous fungi, spreading 0.1 ml inoculum from the serial dilution tubes on the Petri dishes containing Rose-Bengal Streptomycin Agar medium. Twenty three (23) species of fungi namely Penicillium caseicolum, P. commune, P. chrysogenum, P. funiculosum, P. lilacinum, P. olivicolor, P. dimorphosporum, Penicillium sp. I, Penicillium sp. II, Penicillium sp. III, Penicillium sp. IV, Aspergillus flavus, A. fumigatus, A. japonicus, A. niger, A. terreus, A. versicolor, A. wentii, Aspergillus sp. Fusarium sp. Rhizopus sp. Acremonium sp. and Mucor sp. belonging to five genera were recovered from the Lake water samples. Penicillium and Aspergillus were the most dominant genera with a total of 11 and 8 species respectively. The most prevalent species was P. chrysogenum with its occurrence at all sixteen (16) sampling stations and a highest total of seventeen species was recorded at site 16 (Pokhribal Nallah II).[...] Read More.
Keywords: Filamentous fungi, Dal Lake, serial dilution, qualitative.
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Luis Cesar Maffei Sartini Paulillo, Sizenando José de Andrade Junior, Abelmon da Silva Gesteira, Marco Brotto, and Aristóteles Góes-Neto
Abstract: Lignocellulolytic fungi have significant potential as bio-convertors with a particular capacity to degrade recalcitrant natural composts, such as lignin. We generated a cDNA library of a lignocellulolytic fungus, the Phellinus rimosus (Hymenochaetaceae, Basidiomycota), and a typical species of polypore mushroom from the Brazilian semi-arid region. To the best of our knowledge, this is the first report ever to describe the existence of a Ty1-copia retrotransposon in the P. rimosus species. Given the taxonomic complexity in the Brazilian semi-arid region, our findings will be useful as they provide a molecular identity to this important parasite species of the semi-arid region of Brazil.[...] Read More.
Keywords: Lignocellulolytic fungi, biotechnology, Phellinus rimosus, Ty-1 copia, Brazilian semi-arid region, molecular marker.
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M. Jayant*, J. Rashmi, M. Shailendra and Y. Deepesh
Abstract: Cellulases are a group of hydrolytic enzymes capable of degrading cellulose to the smaller glucose units. These enzymes are produced by fungi and bacteria. The solid waste of sugar, paper and industry using baggase, paper waste and cotton waste was fermented by Aspergillus niger and Penicillium chrysogenum in solid state fermentation. There is attempts to transfer the various industrial carbon waste to veterinary proteins depend on microorganisms by using of chemical process. The study indicates that the cellulases obtained from compatible mixed cultures simultaneous mixing of both fungi have more enzyme activity as compared to their pure cultures and other combinations. The fermentation experiments were performed in solid stat fermentation (SSF). Incubation time, carbon sources and initial pH of fermentation medium was optimized with simultaneous mixed culture. It was revealed that the newspaper at pH = 5 and 40°C was the best source of carbon for the enhanced production of cellulase in the compatible mixed culture experiments after 8 days of incubation. Based on the reported results, it may be concluded that industrial carbon waste can be a potential substrate for production of cellulase, incorporation of co-culturing A. niger and P. chrysogenum. The aim of this work is to produce cellulase from waste paper and reduce the pollution.[...] Read More.
Keywords: Aspergillus niger, Penicillium chrysogenum, cellulase, culture.
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S. Rehman*, Tariq Mir, A. Kour, P. H. Qazi, P. Sultan and A. S. Shawl
Abstract: Nodulisporium sp. an endophytic fungus identified by 28s ribosomal gene sequencing isolated from a medicinal plant, Nothapodytes foetida was studied for its in vitro antimicrobial activity. Dual culture studies were carried out for antifungal activity where maximum antagonistic activity was against Alternaria alternata and Colletotrichum gleosporoides. For antibacterial studies, Gram positive and Gram negative human pathogens strains were used. The minimum inhibitory concentration (MIC) of ethyl acetate and methanol fractions of Nodulipsporium showed appreciable growth inhibition mainly active against disease causing Gram positive bacteria.[...] Read More.
Keywords: Nothapodytes foetida, endophytes, antagonism, antimicrobial, Nodulisporium, sp., pathogen, bacteria, minimum inhibitory concentration.
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文章
Idise Okiemute Emmanuel* and Ofiyai Odoyo
Abstract: Wine was produced from pawpaw (Carica papaya) at a ratio of 1:4 for pawpaw must: water in fermentation vessels A to D. Fermentation vessel A pawpaw must contained natural yeast and water; B contained natural yeast from pawpaw and sugar solution; C contained natural yeast, activated baker’s yeast and sugar solution; and D (control) contained sugar solution and Baker’s yeast. Pawpaw wines produced had average values of 3.84, 29.6, 0.628, 0.9950, 0.464, 1.348, 6.66 and 0.54; 3.76, 29.6, 0.631, 1.0036, 0.623, 1.358, 6.89 and 0.37; 3.86, 29.8, 0.718, 0.9994, 0.419, 1.354, 6.32 and 0.78; and 3.33, 29.6, 0.659, 0.9974, 0.216, 1.351, 6.72 and 0.8 for pH, temperature (°C), optical density (at 560 nm), specific gravity, percentage titratable acidity, percentage alcohol (v/v), total aerobic count (log10cfu/ml) and retardation factor (Rf) (cm). Fermentation was carried out for 144 h, and it was observed that malo-lactic fermentation after 48 h was evident. Testing of the wine’s taste showed very little differences in the wines from Recipes A – C, while statistical analyses at 95% confidence level showed no significant differences. The wine from the control had similar taste and characteristics with natural palm wine. Pawpaw wine could thus be produced for immediate consumption, or preserved by refrigeration using Recipes A - C. More research is, however, required to determine the shelf stability of the pawpaw wine.[...] Read More.
Keywords: Pawpaw, flora, fermentation, sugar, wine, flavor, yeast.
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文章
Valerie Gonsalves, Shweta Nayak and Sarita Nazareth*
Abstract: Halophilic mycobiota was isolated from Mandovi estuary and it was dominated by Aspergillus and Penicillium species. Cladosporium and Eurotium were found in lesser numbers while obligate halophiles were found only amongst the aspergilla and they were all identified as Aspergillus penicillioides. Some aspergilli and all the isolates of Penicillium, Cladosporium and Eurotium were facultative halophiles. There were significant differences in growth of each isolate at different salt concentrations. Most of the isolates were euryhaline, having a wide range of salt tolerance; a few were stenohaline, with a narrow range of halotolerance. The isolates were mainly moderate halophiles, with a very few slight halophiles. Isolation of obligate halophilic fungi from polyhaline environment of an estuary is hereby reported for the first time.[...] Read More.
Keywords: Aspergillus penicillioides, obligate halophiles, facultative halophiles.
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