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Zangué S. C. Desobgo, Emmanuel J. Nso* and Dzudie Tenin
Abstract: The action of three technical mashing enzymes (hitempase 2XL, bioglucanase-TX and brewers protease) on yields of extract and free amino nitrogen (FAN) of the worts of mashes of unmalted and malted Madjeru sorghum was modeled and analyzed using the response surface methodology. The analysis showed that increasing amounts of hitempase 2XL considerably increased yields of extract during mashing of unmalted Madjeru sorghum grist. The use of bioglucanase-TX was not indispensable, while Brewers’ protease contributed very little. Increasing amounts of hitempase contributed approximately 45% of the free amino nitrogen, while Brewers’ protease influence amounted to not more than 15%. Bioglucanase’s action was globally nil. Addition of the three enzymes into malted Madjeru sorghum mashes had no significant effect on the yields of extracts and FAN, but the milling operation singularly liberated more than 50% of FAN for both mash types. Optimization of the concerted actions of the three enzymes for extract yield for unmalted Madjeru sorghum mash gave a combination of (1960.5 U; 132.61 BGU and 28.86 mg) for hitempase, bioglucanase and brewers protease respectively). This gave a maximal extract yield of 16.55 °P. This combination was: 2610 U; 0 BGU and 40.44 mg for malted Madjeru sorghum mash, giving a maximal extract yield of 16.35 °P. Optimization for free amino nitrogen for unmalted Madjeru sorghum mash gave a combination of: 3000 U; 0 BGU and 100 mg for hitempase, bioglucanase and brewers protease respectively). This gave maximal FAN of 93.55 mg/L. The combination was: 3000 U; 0 BGU and 100 mg for malted Madjeru sorghum mash, giving a maximal FAN of 144.48 mg/L.[...] Read More.
Keywords: Modeling, technical mashing enzymes, yields of extract, free-amino-nitrogen, Madjeru, optimization.
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文章
I. D. Chukwu, O. O. C. Chukwu*, Chuku A., Israel B., and B. I. Enweani
Abstract: This article describes a study undertaken to evaluate the prevalence of dermatophytoses among one hundred and ten children between the ages of 3 to 14 years in rural primary school children of Barkin-Ladi Local Government Area, in association with the family livestock keeping, family hygiene level, environmental conditions of the schools and identify interventions to improve environmental health. Out of the number sampled, 108 (98.2%) were positive for fungal infections and 2 (1.8%) were negative was found to be highly significant at 5 and 10% level of probability at (P = 0.01 and P = 0.05). Of these 108 fungal isolates, 91(84.3%) were dermatophytes while 17 (15.7%) were non-dermatophytes. Among the pupils investigated, 56.5% fungal isolates were from males while 43.5% from females. From the site of fungal infections on the body of the pupils screened, 90 (83.4%) were from the head (hair) which is significant at 5 and 10% level of probability at (P = 0.01 and P = 0.05) compared to, 13 (12%) from the body trunks and 5 (4.6%) from the face. The dermatophytes isolates were; Trichophyton mentagrophytes, 26 (24%), Trichophyton violaceum, 11 (10.2%) Microsporum audouinii 10 (9.3%), Trichophyton verrucosum 8 (7.4%), Microsporum ferrugineum 8 (7.4%), Microsporum canis 7 (6%), Trichophyton tonsurans 6(5.5%), Trichophyton concentricum 4 (3.7%), Trichophyton schoenleinii 3 (2.8%) and Trichophyton megninii 2 (1.9%) and Trichophyton rubrum 6 (5.5%). The non-dermatophytes were; Aspergillus fumigatus 4 (3.7%), Mucor species 3 (2.8%), Penicillium species 2 (1.9%), Aspergillus niger 2(1.9%), Aspergillus flavus 2(1.9%), Candida albicans 2 (1.9%), Trichoderma species 1 (0.9%) and Alternaria species 1 (0.9%). The results showed the role of family hygiene standards and environmental sanitary quality in dermatophytoses among rural primary school children.[...] Read More.
Keywords: Dermatophytoses, school children, livestock, environmental hygiene.
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M. Moghtader
Abstract: The antifungal effects of the essential oil from Thymus vulgaris L. and comparison with synthetic thymol on Aspergillus niger growth was studied. The chemical composition of the essential oil of T. vulgaris, the aerial parts of this plant which is grown in a village in Kerman Province at full flowering stage in June 2012 were collected. The sample was cleaned, dried in the shade and hydro distillation method was performed for the extraction of essential oil. The main oil content from the plant of T. vulgaris was 2.25% (v/w). Essential oil was analyzed by capillary gas chromatography (GC) using flame ionization (FID) and capillary gas chromatography coupled mass spectrometry (GC/MS) for detection. Thirty-two compounds were identified in the essential oil of T. vulgaris that concluded 99.56% of the total oil. The major components were Thymol (32.67%), P-cymene (16.68%), γ-terpinene (12.65%) and Carvacrol (8.32%). The study of antifungal effects of the oil sample was tested against strain of A. niger (PTCC=5223) fungi by disc diffusion method via average inhibition zone. The results show that essential oil from Thymus plant at 1, 1/2 and 1/4 oil dilutions exhibits strong antifungal activity than Streptomycin sulphate (72% SP) and gentamycin (8 mg/ml) antibiotics on A. niger and that exhibited on strong synthetic thymol was at 10% dilution. The high percentage antifungal activities of Thymus oil are related with thymol is a natural monoterpene phenol as the main compound.[...] Read More.
Keywords: Thymus vulgaris L., Aspergillus niger, essential oil, antifungal activity, fungal growth, thymol.
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Donatha Damian Tibuhwa
Abstract: Traditional taxonomy emphasizes the morphological features to characterize a taxon. Sarcoscypha is a genus in Sarcoscyphaceae family which display wide array of morphological variations. The genus is widespread in northern hemisphere and boreal regions, but also occurs in sub tropical areas and in the southern hemisphere. Both macro and micromorphological features including (ascocarp size, colour, shape, exterior surface of the fruit body, asci size, shape, as well as ascospore size, ends and lipid bodies) were used in a conventional taxonomic analysis of fresh Sarcoscypha material collected from southern hemisphere in Tanzania. Results showed that compared with similar species from northern hemisphere, Tanzanian materials were relatively smaller, smooth, vivid sharp red inside a saucers-shaped ascocarp, sessile to substipitate, microscopically unsheathed ascospores with two lipid bodies, distinctive geographical distribution, and unique season of fructification. Furthermore, a dichotomous identification key constructed for the six close similar species proved that Tanzanian material differed from other close species compared. Therefore based on conventional morphological taxonomy Tanzanian material from Serengeti National Park is described for the first time as a new Ascomycota; Sarcoscypha ololosokwaniensis sp.nov.[...] Read More.
Keywords: Ascocarp, Ascomycota, Sarcoscypha, Serengeti, Tanzania.
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F. C. K. Ocloo* and G. S. Ayernor
Abstract: Alcohol was produced from cassava flour hydrolysate (CFH) with standard glucose and sucrose solutions used as controls. The conversion efficiency of sugar to alcohol, rate of fermentation and types of alcohol produced were determined. The effects of yeast concentrations (5, 15 and 20%) and fermentation time (24, 48, 72, 96 and 120 h) on yield of alcohol from CFH were also studied. Results showed that the maximum carbon dioxide evolved during fermentation was 8.57 g recorded by CFH. The conversion efficiency of sugars to alcohol was 248.4, 99.51 and 95.37% for CFH, standard glucose and sucrose solutions respectively. Alcohol produced was mostly ethanol with traces of methanol. Yeast concentrations were found not to have any significant effect (p > 0.05) on the alcohol yield, however, fermentation time was found to have had a significant effect (p < 0.05) on alcohol yield. The study suggests that high yield of alcohol could be produced from CFH.[...] Read More.
Keywords: Alcohol, cassava flour hydrolysate, fermentation, yield.
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Marcelina R. Nance and William N. Setzer
Abstract: The essential oils from seven different aroma (finishing) varieties of hops (Humulus lupulus L.), “Cascade”, “Hallertauer”, “Northern Brewer”, “Saaz”, “Sterling”, “Vanguard”, and “Willamette”, were obtained by hydrodistillation and analyzed by gas chromatography – mass spectrometry. A total of 98 compounds were identified with myrcene and α-humulene dominating the profiles. “Cascade” and “Northern Brewer” were rich in monoterpene hydrocarbons, chiefly myrcene, while sesquiterpene hydrocarbons, mostly α-humulene, dominated “Hallertauer”, “Saaz”, “Sterling”, and “Vanguard”. “Willamette” had an even distribution of monoterpenes and sesquiterpenes.[...] Read More.
Keywords: Chemical composition, aroma hops, cascade, Hallertauer, Northern Brewer, Saaz, Sterling, Vanguard, Willamette.
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Jayant Mishra*, Deepesh Kumar, Sumeru Samanta and Manoj Kumar Vishwakarma
Abstract: In this study, ethanol produced from fruits of pineapple, orange and sweet lime was investigated. Effect of different constant times [24 and 72 h, and 8 days (in submerged fermentation)]; pH (3.5 to 8.5), temperature (78°C) and autoclave pre-treatment (121°C, 20min) were also studied to improve the yield of ethanol in fruits. Yeast (Saccharomyces cerevisiae and Candida alb cans) are used for fermentation. Fermentation process used solid state fermentation and submerged fermentation methods. The results showed that there is a substantial increase in the quantity of ethanol produced in submerged fermentation as compared to that produced by the solid state fermentation. Optimal ph and temperatures for the better yield of ethanol were 3.5 to 8.5 and 78°C respectively. Autoclave pre treatment protected the samples from contamination and increased the volume of ethanol. Key word: Ethanol, fruits, sugar concentration, Saccharomyces crevasse and Candida albicans.[...] Read More.
Keywords: Ethanol, fruits, sugar concentration, Saccharomyces crevasse and Candida albicans.
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N.S. Afshan*, A. N. Khalid and A. R. Niazi
Abstract: Puccinia opizii on Carex curta is reported here as a new record for Pakistan. Furthermore, Coleosporium lycopi, Cronartium ribicola, Melampsora epitea, Peridermium thomsonii, Phragmidium rosae-moschatae, Puccinia carthami, Puccinia chrysanthemi, Puccinia circaeae, Puccinia graminis, Puccinia komarovii and Uromyces trifolii are additions to the rust flora of Fairy Meadows, Northern Areas, Pakistan.[...] Read More.
Keywords: Byal camp, carex curta, fairy meadows, Hunza, Nanga Parbat, Northern areas.
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V. B. Hosagoudar, J. Thomas and D. K. Agarwal*
Abstract: Two new species namely, SARCINELLA LORANTHACEARUM and SCHIFFNERULA MELIOSMATIS were collected from Peppara and Neyyar Wildlife sanctuaries and Silent Valley National park are described and illustrated in detail.[...] Read More.
Keywords: Black mildews, new species, Kerala, India.
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Ali A. Juwaied, Ahmed Abdulamier Hussain Al-amiery*, Zahraa Abdumuniem and Umar Anaam
Abstract: Sugar cane waste was used as substrates for the production of cellulase enzyme using Aspergillus niger and Tricoderm aviride. From the fermentation studies, sugar cane waste gave the best result with an enzyme activity value of (using A. niger and T. aviride) 2.7 and 2.6 IU/ml respectively. It gave the maximum enzyme activity at about the 10 days of cultivation, suggesting that is the optimum time when the enzyme may be harvested. The fermentation experiments were performed in solid stat fermentation (SSF). Incubation time and initial pH of fermentation medium watts optimized with simultaneous mixed culture. It was revealed that the sugar cane waste at pH = 4.5 and 40°C Aspergillus niger (37°C for T. viride) was the best source of carbon for the enhanced production of cellulase in the compatible culture experiments. Based on the reported results, it may be concluded that sugar cane waste can be a potential substrate for production of cellulase, incorporation of culturing A. niger or T. viride. The aim of this work is to produce cellulase from sugar cane waste and reduce the pollution.[...] Read More.
Keywords: Aspergillus niger, cellulase, culture, sugar cane waste.
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