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R. Shavindra Dias
Abstract: Illicit brew contaminated with Paraquat, bipridyl non -selective contact herbicide, resulting in an epidemic of paraquat poisoning was investigated in Sri Lanka. It was found that the brewers use to hang the paraquat bottle with the lid pierced over the distilling cocktail. Brewers believe that condensed particles act as a catalyst, increasing the concentration and quality of the distillate. Clinical data, biochemical and histopathological data were used to confirm the poison. In-depth interviews and group discussions were used to elucidate the mechanism used by the illicit brewers. Due to an accident the paraquat bottle had slipped into the distilling cocktail resulting in this epidemic of paraquat poisoning.[...] Read More.
Keywords: Paraquat, Illicit, brewers, cocktail.
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João V. B. de Souza*, Alita M. Lima, Eveleise S. de J. Martins, Julia I. Salem
Abstract: The aim of this work was to increase the concentration of substances with anti-mycobacterium activity in culture filtrates obtained from the dematiaceous fungus C10. An experimental design was employed to study the effect of glucose, potato infusion and Senna reticulata infusion. The anti-mycobacterium activity was determined by evaluating the growth of bacteria in culture medium containing “culture filtrate” (the products of the submerged fermentation of the fungus). It was observed that the concentrations of glucose 30 g/L, potato infusion 50% v/v and S. reticulata infusion 0% v/v (a better result was obtained not using S. reticulata infusion) were the best conditions for metabolites production. The influence of each variable was determined and it was possible to produce a mathematical model and a surface response to demonstrate the influence of the studied variables. In conclusion, we note that the culture medium had a great importance in the production of culture filtrate with anti-mycobacterial activity and that the experimental design showed to be a functional statistical tool for studying the influence of culture medium composition.[...] Read More.
Keywords: Culture medium, Senna reticulata, endophytic fungi, experimental design.
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文章
Olu Malomo*, Ogunmoyela O. A. B., Oluwajoba S. O., Adigun M. O. and Daniel ‘Toyosi
Abstract: The type and strain of yeast used in fermentation has a great influence on the taste and character of beer produced. Apart from brewing, sorghum has been used extensively in food industries. The essence of this study is to investigate the behavior of yeast in a sorghum/barley brew and also to investigate the sensory acceptability of such a combination. The study showed that the assessors were unable to detect if there were differences below a 40/60 blend, sorghum and barley mix respectively, beyond which all the organoleptic parameters presented became objectionable.[...] Read More.
Keywords: Organoleptic, fermentation, assessors, brewing, sorghum, barley.
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S. J. Sanei* and S. E. Razavi
Abstract: Olive leaf spot or peacock spot, caused by the fungal pathogen Spilocaea oleagina, can cause reduced growth and yield in olive trees (Olea eurapaea). Investigations were carried out during 2007-2010 to measure the prevalence and severity of olive leaf spot in the northern olive growing regions of Iran. The susceptibility of ten cultivars (Amygdalifolia, Blaidy, Koronakei, Mary, Manzanillo, Mission, Rooghany, Valatolina, Wild olive, Zard) to the disease was assessed. Olive scab was found in all study areas and with the worst affected in high relative humidity (Y= -21.058 + 0.794X, p[...] Read More.
Keywords: Peacock spot, Spilocaea oleagina, disease prevalence, disease severity, Iran.
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Vagner G. Cortez* , Iuri G. Baseia and Rosa Mara B. Silveira
Abstract: Boletales is an order of the subclass Phallomycetidae, which comprises of a wide variety of morphological types of macrofungi, including the boletes and earthballs. In this paper, the gasteroid members of the Boletales from Rio Grande do Sul State, in southern Brazil, were revised. Specimens were collected during the years 2006 to 2009, analyzed macro and microscopically and the collections are preserved at the herbarium ICN. The following taxa were recorded: Rhizopogon roseolus (Rhizopogonaceae), Calostoma zanchianum, Pisolithus arhizus, Scleroderma albidum, Scleroderma bovista, Scleroderma citrinum, Scleroderma dictyosporum, Scleroderma fuscum, Scleroderma laeve and Scleroderma verrucosum (Sclerodermataceae). Scleroderma dictyosporum and S. laeve are reported for the first time from Brazil. A key for the identification of the species of Scleroderma is provided and colour photographs and line drawings of the basidiospores are presented for all taxa studied.[...] Read More.
Keywords: Basidiomycota, calostomataceae, ectomycorrhizal fungi, Eucalyptus, Pinus, pisolithaceae, taxonomy.
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David, O. M.*, Fagbohun, E. D., Oluyege, A. O. and Adegbuyi, A.
Abstract: Nutriceutics potential and physicochemical properties of the oil extract from five species of macrofungi which include Ganoderma lucidium, Pleurotus tuberregium, Termytomyces robustus, Schizophyllum commune and Trametes versicolor were investigated using standard chemical and microbiological methods. The oil was extracted using Soxhlet method of extraction. Disc diffusion and agar dilution methods were used to test for the antibacterial and antifungal properties of the samples respectively. The extracted oils were tested against five clinical bacterial isolates: Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus, Serratia marcescens, Enterococcus faecalis and Escherichia coli. The susceptibility of three fungi genera to the oil samples was also tested. The potency of the extracts oils was determined at different concentrations. All the oils extracted from the mushroom were liquid at room temperature. The acid values of the oils ranged between 0.9 and 6.7 mg KOH/g in T. robustus and T. versicolor respectively. The high iodine values ranged between 39.8 in T. versicolor and 127.0 mg I2/100 g. The saponification value was above 100 mg KOH/g except T. robustus. The aromabiogram of the oils from the mushroom had a pronounced effect on the Gram negative bacteria. Oil from S. commune has the least inhibitory effect on the bacteria. The antifungal assay of P. tuberregium was most effective against Aspergillus parasiticus, followed by that of G. lucidium. The least effective was oil from T. versicolor. The performance of oil from G. lucidium was the best out of all the samples. The inhibitory activities of the oils were concentration dependent. The oils tested were good sources of antimicrobials.[...] Read More.
Keywords: Mushroom, oil, nutriceutics, antimicrobial, pathogens, macrofungi.
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Kathiresan Kandasamy*, Nabeel M. Alikunhi and Manivannan Subramanian
Abstract: Yeasts and other fungi are prevalent in marine and estuarine ecosystems where they play an important role in the food web. Marine yeasts are unique in performing fermentations under high salt concentrations. The mechanism underlying the high salt tolerance involves the ability to accumulate high concentrations of sodium without becoming intoxicated, and the exclusion of excessive sodium from the cytoplasm. Overall, the yeasts play major roles in fermentation, enzyme technology, pollution control, micro sensors, and in some medicinal and medical applications.[...] Read More.
Keywords: Marine yeast, deep sea, estuarine, mangrove, association.
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Zangué S. C. Desobgo, Emmanuel J. Nso* and Dzudie Tenin
Abstract: The action of three technical mashing enzymes (hitempase 2XL, bioglucanase-TX and brewers protease) on yields of extract and free amino nitrogen (FAN) of the worts of mashes of unmalted and malted Madjeru sorghum was modeled and analyzed using the response surface methodology. The analysis showed that increasing amounts of hitempase 2XL considerably increased yields of extract during mashing of unmalted Madjeru sorghum grist. The use of bioglucanase-TX was not indispensable, while Brewers’ protease contributed very little. Increasing amounts of hitempase contributed approximately 45% of the free amino nitrogen, while Brewers’ protease influence amounted to not more than 15%. Bioglucanase’s action was globally nil. Addition of the three enzymes into malted Madjeru sorghum mashes had no significant effect on the yields of extracts and FAN, but the milling operation singularly liberated more than 50% of FAN for both mash types. Optimization of the concerted actions of the three enzymes for extract yield for unmalted Madjeru sorghum mash gave a combination of (1960.5 U; 132.61 BGU and 28.86 mg) for hitempase, bioglucanase and brewers protease respectively). This gave a maximal extract yield of 16.55 °P. This combination was: 2610 U; 0 BGU and 40.44 mg for malted Madjeru sorghum mash, giving a maximal extract yield of 16.35 °P. Optimization for free amino nitrogen for unmalted Madjeru sorghum mash gave a combination of: 3000 U; 0 BGU and 100 mg for hitempase, bioglucanase and brewers protease respectively). This gave maximal FAN of 93.55 mg/L. The combination was: 3000 U; 0 BGU and 100 mg for malted Madjeru sorghum mash, giving a maximal FAN of 144.48 mg/L.[...] Read More.
Keywords: Modeling, technical mashing enzymes, yields of extract, free-amino-nitrogen, Madjeru, optimization.
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I. D. Chukwu, O. O. C. Chukwu*, Chuku A., Israel B., and B. I. Enweani
Abstract: This article describes a study undertaken to evaluate the prevalence of dermatophytoses among one hundred and ten children between the ages of 3 to 14 years in rural primary school children of Barkin-Ladi Local Government Area, in association with the family livestock keeping, family hygiene level, environmental conditions of the schools and identify interventions to improve environmental health. Out of the number sampled, 108 (98.2%) were positive for fungal infections and 2 (1.8%) were negative was found to be highly significant at 5 and 10% level of probability at (P = 0.01 and P = 0.05). Of these 108 fungal isolates, 91(84.3%) were dermatophytes while 17 (15.7%) were non-dermatophytes. Among the pupils investigated, 56.5% fungal isolates were from males while 43.5% from females. From the site of fungal infections on the body of the pupils screened, 90 (83.4%) were from the head (hair) which is significant at 5 and 10% level of probability at (P = 0.01 and P = 0.05) compared to, 13 (12%) from the body trunks and 5 (4.6%) from the face. The dermatophytes isolates were; Trichophyton mentagrophytes, 26 (24%), Trichophyton violaceum, 11 (10.2%) Microsporum audouinii 10 (9.3%), Trichophyton verrucosum 8 (7.4%), Microsporum ferrugineum 8 (7.4%), Microsporum canis 7 (6%), Trichophyton tonsurans 6(5.5%), Trichophyton concentricum 4 (3.7%), Trichophyton schoenleinii 3 (2.8%) and Trichophyton megninii 2 (1.9%) and Trichophyton rubrum 6 (5.5%). The non-dermatophytes were; Aspergillus fumigatus 4 (3.7%), Mucor species 3 (2.8%), Penicillium species 2 (1.9%), Aspergillus niger 2(1.9%), Aspergillus flavus 2(1.9%), Candida albicans 2 (1.9%), Trichoderma species 1 (0.9%) and Alternaria species 1 (0.9%). The results showed the role of family hygiene standards and environmental sanitary quality in dermatophytoses among rural primary school children.[...] Read More.
Keywords: Dermatophytoses, school children, livestock, environmental hygiene.
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文章
M. Moghtader
Abstract: The antifungal effects of the essential oil from Thymus vulgaris L. and comparison with synthetic thymol on Aspergillus niger growth was studied. The chemical composition of the essential oil of T. vulgaris, the aerial parts of this plant which is grown in a village in Kerman Province at full flowering stage in June 2012 were collected. The sample was cleaned, dried in the shade and hydro distillation method was performed for the extraction of essential oil. The main oil content from the plant of T. vulgaris was 2.25% (v/w). Essential oil was analyzed by capillary gas chromatography (GC) using flame ionization (FID) and capillary gas chromatography coupled mass spectrometry (GC/MS) for detection. Thirty-two compounds were identified in the essential oil of T. vulgaris that concluded 99.56% of the total oil. The major components were Thymol (32.67%), P-cymene (16.68%), γ-terpinene (12.65%) and Carvacrol (8.32%). The study of antifungal effects of the oil sample was tested against strain of A. niger (PTCC=5223) fungi by disc diffusion method via average inhibition zone. The results show that essential oil from Thymus plant at 1, 1/2 and 1/4 oil dilutions exhibits strong antifungal activity than Streptomycin sulphate (72% SP) and gentamycin (8 mg/ml) antibiotics on A. niger and that exhibited on strong synthetic thymol was at 10% dilution. The high percentage antifungal activities of Thymus oil are related with thymol is a natural monoterpene phenol as the main compound.[...] Read More.
Keywords: Thymus vulgaris L., Aspergillus niger, essential oil, antifungal activity, fungal growth, thymol.
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