Search for Articles:
Journal:
Subject:

Search Filter

Years
Between: -
Article Types
Search Results (2768)
Order
page
文章
Sadeghi Heibatollah*, Nikbakht Mohammad Reza; Ghaitasi Izadpanah and Sabzali Sohailla
Abstract: The purpose of this study was to evaluate the hepatoprotective activity of hydroalcholic extract of Cichorium intybus (Cichorium intybus) using a carbon tetrachloride (CCl4) - induced liver injury in rats. The leaf extract at oral dosage of 200, 400 and 500 mg/kg exhibited significant (P[...] Read More.
Keywords: Cichorium intybus, hepatoprotective activity, carbon tetrachloride, aspartate aminotransferase, alanine aminitransferase, alkaline phosphatase.
Full text
Views:855
Download:766
文章
Airong Li, Yue Zhu, Liang Xu, Wenqing Zhu and Xingjun Tian*
Abstract: The aim of this work was to determine the sensitivity among compounds in common use for detecting laccase activity. In this study, three assay procedures to measure laccase activity of Trametes sp. were performed in Kirk’s basal salts mediums of three dyes. In the assay methods, three substrates were employed, which were 2,2©-azinobis-(3-ethylbenzthiazoline-6-sulfonate) (ABTS), -dianisidine and guaiacol. The results indicate that laccase activity used ABTS as a substrate was significantly higher than the other two. Therefore, the ABTS method is recommended while detecting laccase activity.[...] Read More.
Keywords: ABTS,    -dianisidine, guaiacol, laccase activity, Trametes sp.
Full text
Views:764
Download:771
文章
L. A. Nwogu*, C. U. Igwe and A. A. Emejulu
Abstract: The effects of aqueous extract of Landolphia owariensis leaves on the biochemical indices of liver function were investigated in Wister strain of albino rats. Preliminary phytochemical analysis of the plant leaves showed the presence of tannins, alkaloids, flavonoids and saponins. Acute toxicity tests of the extract gave an LD50 of 3370 mg/kg. Liver function tests revealed that the serum activities of alanine aminotransferrase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP), as well as the concentrations of total protein and albumin were not significantly (p>0.05) affected by the oral administration of the extract. However, bilirubin and haemoglobin concentrations decreased significantly (p[...] Read More.
Keywords: Landolphia owariensis, hepatotoxicity, LD50, haemoglobin.
Full text
Views:773
Download:755
文章
Narendra M., Kavitha G., Padmavathi P., Helah Kiranmai A. and Varadacharyulu N. C.*
Abstract: Erythrocyte membranes from twelve human volunteers exposed regularly to allethrin, a mosquito repellent of type-I pyrethroid, were analyzed for cholesterol (C), phospholipids (P), and individual phospholipid classes to assess changes induced by this toxicant. A decrease in C and P moieties with no change in C: P ratio was observed with allethrin exposure. A significant reduction in the amount of phosphotidyl serine (PS) was noticed indicating that PS is an allethrin sensitive phospholipid species. Furthermore, decreased red cell membrane lipid peroxidation (LPO) and with no change in osmotic haemolysis of erythrocyte was observed. Increased plasma and red cell nitrate and nitrite were evident suggesting that the bioavailability of nitric oxide may have rendered tolerance to erythrocyte membrane by protecting the cell from haemolysis and oxidative damage due to its free radical scavenging and antioxidant effects. Keywords: Allethrin, nitric oxide, osmotic haemolysis, phosphatidyl serine, rbc biochemical changes.[...] Read More.
Keywords: Allethrin, nitric oxide, osmotic haemolysis, phosphatidyl serine, rbc biochemical changes.
Full text
Views:827
Download:737
文章
B. Meena*, R. Anbin Ezhilan, R. Rajesh, A. Sheik Hussain, B. Ganesan, R. Anandan
Abstract: We have examined the protective effect of ethanol extract of Sargassum polycystum on hepatic antioxidant status in D-galactosamine-induced hepatitis in rats. Levels of diagnostic marker enzymes [alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and creatine phosphokinase (CPK)] in plasma, lipid peroxides, reduced glutathione and the activities of glutathione dependent antioxidant enzymes [glutathione peroxidase (GPx) and glutathione- S-transferase (GST)] and antiperoxidative enzymes [catalase (CAT) and superoxide dismutase (SOD)] in the liver tissue were determined. Prior oral administration of S. polycystum extract [125mg/kg bodyweight/day for 15 days] considerably (P[...] Read More.
Keywords: Sargassum polycystum, D-galactosamine-induced hepatitis, diagnostic marker enzymes, lipid peroxidation, antioxidant enzymes
Full text
Views:704
Download:783
文章
Hanaa H. Abd El-Baky*, Hussein M. M. and Game S. El-Baroty
Abstract: Effect of irrigation bread wheat plants (Triticum aestivum L., cv. Giza 94) with sea water (10 and 20% v/v), spraying with microalgae extracts obtained from Chlorella ellipoida and Spirulina maxima (5 gL-1 dry weight in 0.1% Tween solution) cultivated under normal and stress conditions were studied. Some plant bioregulators (BRGs, ascorbic acid and benzyl adenine, at 200 ppm) at the vegetative growth stage on photosynthetic pigments, antioxidant components, activity of some antioxidant enzymes, lipid peroxidation products, growth parameters, mineral content and economic yield were estimated. Irrigation of wheat plants with sea water led to an increase in Na+ ion, activities of antioxidant enzymes, superoxide dismutase, ascorbate peroxidase and total peroxidase, and TBARs components. In contrast, the contents of photosynthetic pigments and yield components were reduced. Furthermore, the overall growth of wheat plants was interrupted by irrigation with sea water (10 and 20%) and the effect was pronounced at higher level (20%). Application of BRGs had a slight effect on plant growth, antioxidant behavior and activity of antioxidant enzymes in plants irrigation with sea water compared with that in stressed wheat plants. Application of algal extracts significantly increased the contents of total chlorophyll and antioxidant phenomenon. In additional, application of algal extracts exhibited strong positive correlation with increase in fresh weight (FW), grain weight and yield components. It is concluded that productive purpose of wheat crop by mean of brackish water (at 20 v/v level) is possible under a level of economical value through its application of algal extracts. Keywords: Microalgae, sea water, wheat, salinity stress, antioxidant systems[...] Read More.
Keywords: Microalgae, sea water, wheat, salinity stress, antioxidant systems
Full text
Views:759
Download:795
文章
*Manal F. Ismail, Shohda A. EL-Maraghy, Nermin A.H. Sadik
Abstract: The present study was carried out to evaluate the anti-arthritic nature of evening primrose oil (EPO) rich in -linolenic acid (GLA), on adjuvant -induced arthritic rats. Results were compared to those of diclofenac sodium, a reference standard non-steroidal anti-inflammatory drug. Arthritis was induced by subcutaneous injection of complete Freund’s adjuvant (CFA) in the right hind paw of male Wistar rats. EPO (5 g/kg b.w.) was orally administered from day 0 till day 28, while diclofenac sodium (5 mg/kg b.w.) was orally given from day 10 to day 28 after adjuvant injection. In the arthritic group, the results revealed significant decrease in body weight together with increased spleen weight, increase in serum immunoglobulin G (IgG), immunoglobulin M (IgM) and tumor necrosis factor- (TNF- ) levels whereas serum interleukin-4 (IL-4) level was decreased. Serum N-acetyl- -D-glucosaminidase (NAG) and - glucuronidase enzyme activities were also elevated. Splenic antioxidant status was suppressed as manifested by increased malondialdehyde (MDA) level, decreased glutathione (GSH) content along with decreased enzymatic activity of glutathione peroxidase (GSHPx), glutathione reductase (GR) and glutathione-S-transferase (GST). Moreover, the splenic activity of the pathophysiological enzymes, - glutamyltransferase ( -GT) and lactate dehydrogenase (LDH) was enhanced. Oral administration of EPO exerted a significant elevation in serum IgG and IgM levels. In addition, normalization of body weight, serum IL -4 and TNF- levels together with serum NAG, -glucuronidase and splenic LDH enzyme activities were observed. It could be suggested that administration of plant oils rich in GLA may provide a way to modulate the inflammatory response through alteration of certain inflammatory indices.[...] Read More.
Keywords: Adjuvant-induced arthritis, evening primrose oil, immunoglobulins, IL- 4, TNF- , rat.
Full text
Views:782
Download:725
文章
Neeraj Verma, Bhaskar C. Behera*, Anjali Sonone and Urmila Makhija
Abstract: The symbionts of lichen species Arthothelium awasthii, Heterodermia podocarpa, and Parmotrema tinctorum were isolated and cultured on various nutrient media incubated in a growth chamber. Methanolic extracts of one year old symbiont cultures were taken for evaluating their potential to inhibit lipid peroxidation and tyrosinase enzyme activity. The extracts of all cultured lichen-symbionts have shown a concentration-time dependent inhibition of lipid peroxidation and tyrosinase activities. IC50 values for inhibition of lipid peroxidation by culture-extracts were 15.7 µg for A. awasthii, 12.68 µg for H. podocarpa and 11.47 µg for P. tinctorum. Testing the impact of the extracts on tyrosinase activity, we found IC50 of 8.71 µg (A. awasthii), 14.55 µg (H. podocarpa) and 12.44 µg (P. tinctorum). According to their IC50 the tested extracts turned out to be more effective for the tested parameters than the standard antioxidant Trolox (IC50 for lipid peroxidation 16.13 µg) and Kojic acid tyrosinase inhibitor (IC50 for tyrosinase inhibition 17.63 µg). The results suggest possible applications of lichen substances in the extracts of A. awasthii, H. podocarpa, and P. tinctorum symbionts as natural tyrosinase inhibitors. Keyword: Lichen culture, Tyrosinase inhibition[...] Read More.
Keywords: Lichen culture, Tyrosinase inhibition
Full text
Views:741
Download:757
文章
*Nermin A.H. Sadik, Shohda A. EL-Maraghy, Manal F. Ismail
Abstract: Nitrosamine compounds are known hepatic carcinogens. This study was designed to study the efficacy of dietary supplementation with blueberries (BB) on diethylnitrosamine (DEN)-initiated hepatocarcinogenesis in male wistar rats. Rats were divided into three groups. The first group served as normal control group, the second group received DEN at a dose of 10 mg/kg body weight five times a week for 15 weeks. The third one received DEN as in DEN-treated group simultaneously with 4% BB-supplemented diet. The results showed that BB caused significant decrease in the elevated serum levels of -fetoprotein (AFP), homocysteine (Hcy) along with levels of glutathione(GSH), deoxyribonucleic acid (DNA), ribonucleic acid (RNA)and activity of glutathione reductase (GR) in liver. Normalization of elevated 2-macroglobulin ( 2M) and total antioxidant capacity (TAC) levels in serum, hepatic glutathione-S-transferase (GST), glutathione peroxidase (GPx) activities and liver weight was achieved whereas body weight was significantly decreased. Moreover, no significant change was observed in elevated relative liver weight, hepatic glucose-6-P-dehydrogenase (G6PD), lactate dehydrogenase (LDH) along with serum aminotransferases, alkaline phosphatase (ALP) and - glutamyltransferase ( -GT) activities. Significant increase in reduced hepatic activity of xanthine oxidase (XO) was achieved and histopathological damage was minimized in BB-treated group. It is suggested that BB suppress DEN- induced hepatocarcinogenesis and could be developed as a promising chemopreventive natural supplement for liver cancer.[...] Read More.
Keywords: Blueberries, diethylnitrosamine, hepatocarcinogenesis, 2-macroglobulin, -fetoprotein, homocysteine, glutathione, rats.
Full text
Views:722
Download:720
文章
Mukunda Chethankumar and Leela Srinivas*
Abstract: We determined the ability of mixed gangliosides (16% GD1b, 19% GT1b, 21% GM1, and 40% GD1a) and individual gangliosides GM1 and GD1b to modulate the NV-PLA2 induced human erythrocyte ghost membrane damage. CM-Sephadex purification of crude Naja naja venom yielded eight peaks of which peak VII, a major phospholipase A2 (NV-PLA2) accounted for 22% of the total protein recovered and 8% of the total PLA2 activity recovered. The membrane damage induced by NV-PLA2 was assessed by measuring the decrease in the relative intensity of fluorescence using cis–parinaric acid (PnA) as a monitor molecule. The RBC membranes isolated from healthy human blood showed 72% damage on treatment with NV-PLA2 (2 mg) when compared to untreated membranes. Mixed gangliosides (18 nM) and GM1 (15 nM) offered 81 and 86% protection respectively, whereas GD1b (20 nM) did not show significant protection. Analysis of membrane bound Na+K+ and Ca2+Mg2+ ATPase indicated a 3 fold and 2 folds decrease in their activities on NV-PLA2 treatment when compared to untreated membranes. Mixed gangliosides restored the Na+K+ ATPase activity by 78%, whereas GM1 and GD1b offered 74 and 52% restoration respectively. The Ca2+Mg 2+ ATPase activity was restored by 80 and 81% with mixed gangliosides and GM1 respectively. GD1b showed only 50% restoration. Mixed gangliosides and GM1 exhibited a significant dose dependent inhibition of NV-PLA2 activity when compared to GD1b. The data show that mixed gangliosides and GM1 were effective in modulating NV-PLA2 induced erythrocyte membrane damage than GD1b.[...] Read More.
Keywords: Gangliosides, NV-PLA2, PnA, gangliosides, erythrocyte membrane, Na+ K+ ATPase, Ca2+ Mg2+ ATPase.
Full text
Views:732
Download:739
Submit Your Manuscript Now