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Narendra M., Kavitha G., Padmavathi P., Helah Kiranmai A. and Varadacharyulu N. C.*
Abstract: Erythrocyte membranes from twelve human volunteers exposed regularly to allethrin, a mosquito repellent of type-I pyrethroid, were analyzed for cholesterol (C), phospholipids (P), and individual phospholipid classes to assess changes induced by this toxicant. A decrease in C and P moieties with no change in C: P ratio was observed with allethrin exposure. A significant reduction in the amount of phosphotidyl serine (PS) was noticed indicating that PS is an allethrin sensitive phospholipid species. Furthermore, decreased red cell membrane lipid peroxidation (LPO) and with no change in osmotic haemolysis of erythrocyte was observed. Increased plasma and red cell nitrate and nitrite were evident suggesting that the bioavailability of nitric oxide may have rendered tolerance to erythrocyte membrane by protecting the cell from haemolysis and oxidative damage due to its free radical scavenging and antioxidant effects. Keywords: Allethrin, nitric oxide, osmotic haemolysis, phosphatidyl serine, rbc biochemical changes.[...] Read More.
Keywords: Allethrin, nitric oxide, osmotic haemolysis, phosphatidyl serine, rbc biochemical changes.
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B. Meena*, R. Anbin Ezhilan, R. Rajesh, A. Sheik Hussain, B. Ganesan, R. Anandan
Abstract: We have examined the protective effect of ethanol extract of Sargassum polycystum on hepatic antioxidant status in D-galactosamine-induced hepatitis in rats. Levels of diagnostic marker enzymes [alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and creatine phosphokinase (CPK)] in plasma, lipid peroxides, reduced glutathione and the activities of glutathione dependent antioxidant enzymes [glutathione peroxidase (GPx) and glutathione- S-transferase (GST)] and antiperoxidative enzymes [catalase (CAT) and superoxide dismutase (SOD)] in the liver tissue were determined. Prior oral administration of S. polycystum extract [125mg/kg bodyweight/day for 15 days] considerably (P[...] Read More.
Keywords: Sargassum polycystum, D-galactosamine-induced hepatitis, diagnostic marker enzymes, lipid peroxidation, antioxidant enzymes
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Hanaa H. Abd El-Baky*, Hussein M. M. and Game S. El-Baroty
Abstract: Effect of irrigation bread wheat plants (Triticum aestivum L., cv. Giza 94) with sea water (10 and 20% v/v), spraying with microalgae extracts obtained from Chlorella ellipoida and Spirulina maxima (5 gL-1 dry weight in 0.1% Tween solution) cultivated under normal and stress conditions were studied. Some plant bioregulators (BRGs, ascorbic acid and benzyl adenine, at 200 ppm) at the vegetative growth stage on photosynthetic pigments, antioxidant components, activity of some antioxidant enzymes, lipid peroxidation products, growth parameters, mineral content and economic yield were estimated. Irrigation of wheat plants with sea water led to an increase in Na+ ion, activities of antioxidant enzymes, superoxide dismutase, ascorbate peroxidase and total peroxidase, and TBARs components. In contrast, the contents of photosynthetic pigments and yield components were reduced. Furthermore, the overall growth of wheat plants was interrupted by irrigation with sea water (10 and 20%) and the effect was pronounced at higher level (20%). Application of BRGs had a slight effect on plant growth, antioxidant behavior and activity of antioxidant enzymes in plants irrigation with sea water compared with that in stressed wheat plants. Application of algal extracts significantly increased the contents of total chlorophyll and antioxidant phenomenon. In additional, application of algal extracts exhibited strong positive correlation with increase in fresh weight (FW), grain weight and yield components. It is concluded that productive purpose of wheat crop by mean of brackish water (at 20 v/v level) is possible under a level of economical value through its application of algal extracts. Keywords: Microalgae, sea water, wheat, salinity stress, antioxidant systems[...] Read More.
Keywords: Microalgae, sea water, wheat, salinity stress, antioxidant systems
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*Manal F. Ismail, Shohda A. EL-Maraghy, Nermin A.H. Sadik
Abstract: The present study was carried out to evaluate the anti-arthritic nature of evening primrose oil (EPO) rich in -linolenic acid (GLA), on adjuvant -induced arthritic rats. Results were compared to those of diclofenac sodium, a reference standard non-steroidal anti-inflammatory drug. Arthritis was induced by subcutaneous injection of complete Freund’s adjuvant (CFA) in the right hind paw of male Wistar rats. EPO (5 g/kg b.w.) was orally administered from day 0 till day 28, while diclofenac sodium (5 mg/kg b.w.) was orally given from day 10 to day 28 after adjuvant injection. In the arthritic group, the results revealed significant decrease in body weight together with increased spleen weight, increase in serum immunoglobulin G (IgG), immunoglobulin M (IgM) and tumor necrosis factor- (TNF- ) levels whereas serum interleukin-4 (IL-4) level was decreased. Serum N-acetyl- -D-glucosaminidase (NAG) and - glucuronidase enzyme activities were also elevated. Splenic antioxidant status was suppressed as manifested by increased malondialdehyde (MDA) level, decreased glutathione (GSH) content along with decreased enzymatic activity of glutathione peroxidase (GSHPx), glutathione reductase (GR) and glutathione-S-transferase (GST). Moreover, the splenic activity of the pathophysiological enzymes, - glutamyltransferase ( -GT) and lactate dehydrogenase (LDH) was enhanced. Oral administration of EPO exerted a significant elevation in serum IgG and IgM levels. In addition, normalization of body weight, serum IL -4 and TNF- levels together with serum NAG, -glucuronidase and splenic LDH enzyme activities were observed. It could be suggested that administration of plant oils rich in GLA may provide a way to modulate the inflammatory response through alteration of certain inflammatory indices.[...] Read More.
Keywords: Adjuvant-induced arthritis, evening primrose oil, immunoglobulins, IL- 4, TNF- , rat.
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Neeraj Verma, Bhaskar C. Behera*, Anjali Sonone and Urmila Makhija
Abstract: The symbionts of lichen species Arthothelium awasthii, Heterodermia podocarpa, and Parmotrema tinctorum were isolated and cultured on various nutrient media incubated in a growth chamber. Methanolic extracts of one year old symbiont cultures were taken for evaluating their potential to inhibit lipid peroxidation and tyrosinase enzyme activity. The extracts of all cultured lichen-symbionts have shown a concentration-time dependent inhibition of lipid peroxidation and tyrosinase activities. IC50 values for inhibition of lipid peroxidation by culture-extracts were 15.7 µg for A. awasthii, 12.68 µg for H. podocarpa and 11.47 µg for P. tinctorum. Testing the impact of the extracts on tyrosinase activity, we found IC50 of 8.71 µg (A. awasthii), 14.55 µg (H. podocarpa) and 12.44 µg (P. tinctorum). According to their IC50 the tested extracts turned out to be more effective for the tested parameters than the standard antioxidant Trolox (IC50 for lipid peroxidation 16.13 µg) and Kojic acid tyrosinase inhibitor (IC50 for tyrosinase inhibition 17.63 µg). The results suggest possible applications of lichen substances in the extracts of A. awasthii, H. podocarpa, and P. tinctorum symbionts as natural tyrosinase inhibitors. Keyword: Lichen culture, Tyrosinase inhibition[...] Read More.
Keywords: Lichen culture, Tyrosinase inhibition
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*Nermin A.H. Sadik, Shohda A. EL-Maraghy, Manal F. Ismail
Abstract: Nitrosamine compounds are known hepatic carcinogens. This study was designed to study the efficacy of dietary supplementation with blueberries (BB) on diethylnitrosamine (DEN)-initiated hepatocarcinogenesis in male wistar rats. Rats were divided into three groups. The first group served as normal control group, the second group received DEN at a dose of 10 mg/kg body weight five times a week for 15 weeks. The third one received DEN as in DEN-treated group simultaneously with 4% BB-supplemented diet. The results showed that BB caused significant decrease in the elevated serum levels of -fetoprotein (AFP), homocysteine (Hcy) along with levels of glutathione(GSH), deoxyribonucleic acid (DNA), ribonucleic acid (RNA)and activity of glutathione reductase (GR) in liver. Normalization of elevated 2-macroglobulin ( 2M) and total antioxidant capacity (TAC) levels in serum, hepatic glutathione-S-transferase (GST), glutathione peroxidase (GPx) activities and liver weight was achieved whereas body weight was significantly decreased. Moreover, no significant change was observed in elevated relative liver weight, hepatic glucose-6-P-dehydrogenase (G6PD), lactate dehydrogenase (LDH) along with serum aminotransferases, alkaline phosphatase (ALP) and - glutamyltransferase ( -GT) activities. Significant increase in reduced hepatic activity of xanthine oxidase (XO) was achieved and histopathological damage was minimized in BB-treated group. It is suggested that BB suppress DEN- induced hepatocarcinogenesis and could be developed as a promising chemopreventive natural supplement for liver cancer.[...] Read More.
Keywords: Blueberries, diethylnitrosamine, hepatocarcinogenesis, 2-macroglobulin, -fetoprotein, homocysteine, glutathione, rats.
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Mukunda Chethankumar and Leela Srinivas*
Abstract: We determined the ability of mixed gangliosides (16% GD1b, 19% GT1b, 21% GM1, and 40% GD1a) and individual gangliosides GM1 and GD1b to modulate the NV-PLA2 induced human erythrocyte ghost membrane damage. CM-Sephadex purification of crude Naja naja venom yielded eight peaks of which peak VII, a major phospholipase A2 (NV-PLA2) accounted for 22% of the total protein recovered and 8% of the total PLA2 activity recovered. The membrane damage induced by NV-PLA2 was assessed by measuring the decrease in the relative intensity of fluorescence using cis–parinaric acid (PnA) as a monitor molecule. The RBC membranes isolated from healthy human blood showed 72% damage on treatment with NV-PLA2 (2 mg) when compared to untreated membranes. Mixed gangliosides (18 nM) and GM1 (15 nM) offered 81 and 86% protection respectively, whereas GD1b (20 nM) did not show significant protection. Analysis of membrane bound Na+K+ and Ca2+Mg2+ ATPase indicated a 3 fold and 2 folds decrease in their activities on NV-PLA2 treatment when compared to untreated membranes. Mixed gangliosides restored the Na+K+ ATPase activity by 78%, whereas GM1 and GD1b offered 74 and 52% restoration respectively. The Ca2+Mg 2+ ATPase activity was restored by 80 and 81% with mixed gangliosides and GM1 respectively. GD1b showed only 50% restoration. Mixed gangliosides and GM1 exhibited a significant dose dependent inhibition of NV-PLA2 activity when compared to GD1b. The data show that mixed gangliosides and GM1 were effective in modulating NV-PLA2 induced erythrocyte membrane damage than GD1b.[...] Read More.
Keywords: Gangliosides, NV-PLA2, PnA, gangliosides, erythrocyte membrane, Na+ K+ ATPase, Ca2+ Mg2+ ATPase.
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Oladipo GS*, Olabiyi O, Oremosu AA, Noronha CC, Okanlawon AO and Paul CU.
Abstract: Dermatoglyphic analysis of 90 Sickle-cell Anaemia cases and 90 normal subjects was carried out in this study. It involved the digital patterns, ATD angle, A – B ridge count, axial triradius, digital triradius and palmar crease on the hands. 58.44% of the digital patterns in sickle-cell cases were ulnar loop as against 60.14% in the normals. The percentage of Whorl, arch and radial loop in Sickle-Cell group were 31.64%, 8.23% and 2.98% respectively as against 27.47%, 10.43% and 1.98% in the normals. The mean ATD values were 41o and 40o for the normal and sickle-cell groups respectively. The mean A – B ridge counts was 33.1 in sickle-cell group and 33.5 in the normals. No axial triradius was found in t iii (along the distal transverse crease) position in both groups. Most of the triradii were found in position ti (along or close to the thenar crease) in both groups. Only 3.6% of normals and 2.9% sickle-cell cases were found in tii (along the proximal transverse crease) position. The mode of the frequency distribution of digital triradii of normals was 13 while they were 11, 14 and 15 for sickle-cell group. The means were 11.89 and 12.32 respectively. No Simean crease was found in both groups; however, 2.2% of the 90 sickle-cell cases had Sidney creases. The above-mentioned values were not statistically different when the two groups were subjected to appropriate statistical tests.[...] Read More.
Keywords: Dermatoglyphics, sickle-cell anaemia, Nigeria.
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Agnes M. Nandutu, Mike Clifford* and Nazlin K. Howell
Abstract: Sweet potato varieties: Nsp, Tz and Spk obtained from Uganda were used in this study. Several phenolic compounds were identified on the basis of their mass spectra in full scan mode and the pattern of their fragmentation. The major compounds found were caffeoylquinic acid, dicaffeoylquinic acid, coumaroylquinic acid, feruloylquinic acid and caffeoylferuloylquinic acids. This is the first time that caffeoylferuloylquinic isomers have been identified in sweet potato samples.[...] Read More.
Keywords: Sweet potato, phenolic compounds, chlorogenic acids
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Nwodo-Chinedu, S.*, Okochi, V. I., Smith, H. A., Okafor, U. A., Onyegeme-Okerenta, B. M. and Omidiji, O.
Abstract: The effects of glucose, crystalline cellulose and sawdust of Mitragyna cilata on the growth and cellulase production, inferred from cellulase (EC 3. 2. 1. 4) activity, of Penicillium chrysogenum PCL501 was determined. Glucose-containing media gave the highest mycelia weight of 1.78 mg mL-1 in 120 h of incubation. This is about 3.5 – 4.5 times the maximum weights of 0.51 and 0.40 mg mL-1 respectively obtained from the cultures containing cellulose and sawdust. The cultures containing crystalline cellulose and sawdust produced extracellular protein with cellulase (EC 3. 2. 1. 4) activity whereas glucose-containing cultures yielded very low protein and no significant cellulase activity. Maximum protein content of 0.02, 0.13 and 0.46 mg mL-1 respectively were obtained from the cultures containing glucose, cellulose and sawdust. Peak cellulase activity values of 100.0 and 92.2 Units L-1 respectively were obtained for the cultures containing cellulose and sawdust. There is a correlation between the protein released and cellulase activity of the culture filtrates. P. chrysogenum PCL501 produces extracellular proteins with significant cellulase activity in media containing cellulose and sawdust but not in glucose- containing medium. Sawdust is indicated as a good inducer of cellulase activity in the organism. The waste cellulosic material can be used as low-cost carbon source for commercial cellulase production.[...] Read More.
Keywords: P. chrysogenum PCL501, growth, cellulase activity, glucose, cellulosic materials.
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