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S. K. Reshmi, E. Sathya and P. Suganya Devi*
Abstract: Many plant- derived molecules have shown a promising effect in therapeutics. Among the plants investigated to date, one showing enormous potential is the Piperaceae. The present study aimed to extract the phytochemical compounds in different solvent system in Piper longum, Piper nigrum and Piper cubeba as well as testing their antibacterial and antitumour activity. HPTLC analysis of P. nigrum sample showed six alkaloid bands two alkaloid bands were similar to Piperine standard 1 and 2, the other alkaloid may be piperidine, piperettine and piperanine. P. longum sample contain three alkaloid bands one band was similar to Piperine standard 1, the other may be piperlongumine and piperlonguminine and no alkaloid band was found in P. cubeba. The anti bacterial activity was tested against gram positive and negative organism using agar well diffusion method. High activity was found in P. nigrum ethanol extract against the organism Salmonella Typhii. The alkaloid piperdine was purified by refluxion method to check the antitumour activity which shows 51.38% of inhibition at 5 µg/ml concentration that conforms the compound piperidine to be used as anticancer drug for further mechanistic works.[...] Read More.
Keywords: Alkaloids, piperine, HPTLC, antibacterial activity, purification, piperidine, HEp2 cell lines.
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Kamlesh Jayantilal Wadher*, Rajendra Baliram Kakde and Milind Janrao Umekar
Abstract: Metformin hydrochloride is recommended globally as first line therapy due to its favorable profile on morbidity and mortality associated with type- 2 diabetes mellitus. However, limitations of multiple dosing and risk of triggering gastrointestinal symptoms make its dose optimization difficult. The present study was designed to develop the oral sustained release metformin hydrochloride tablet formulation using lipophilic waxes viz. hydrogenated castor oil, stearic acid and glyceryl monostearate either alone or their combinations. The in vitro dissolution study was carried out using USP 22 apparatus I, basket method. The drug release kinetics demonstrated that hydrogenated castor oil sustained the release of metformin greater than stearic acid and glyceryl monostearate when used alone. Combination of hydrogenated castor oil with stearic acid (1:1) sustained the drug release (75.69 ± 0.76%) greater than hydrogenated castor oil with glyceryl monostearate (86.45 ± 0.96%) and stearic acid with glyceryl monostearate (92.29 ± 0.76%) combinations. Kinetic modeling of in-vitro dissolution profiles revealed that metformin release ranges from diffusion controlled or Fickian transport to anomalous type or non-Fickian transport mechanisms. Applying Korsmeyer equation to in-vitro drug release data indicated that diffusion along with erosion could be the mechanism of drug release.[...] Read More.
Keywords: Hydrogenated castor oil, stearic acid, glyceryl monosterate, metformin matrix tablet, release kinetics.
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Daniel Motlhanka*, Peter Houghton, Ana Miljkovic-Brake and Solomon Habtemariam
Abstract: A novel ursane-type pentacyclic triterpene glycoside, 1b,2b,3b-trihydoxy-urs-12-ene-23-oic-rhamnoside was isolated from the resin of Commiphora glandulosa Schinz by bioassay guided fractionation. Crude aqueous and chloroform extracts of the resin as well as the isolated compound exhibited good in vitro antibacterial activity against gram positive bacteria (Bacillus subtilis: NCTC 10073), (Clostridium perfringens: NCTC 8237), (Staphylococcus aureus: NCIMB 9518) and also drug resistant strains (S.aureus:XU212-tetracycline resistant strain), (S. aureus SA1199B-norfloxacin resistant strain). There was no observed antimicrobial activity against gram negative bacteria (Escherichia coli: NCTC 9002), (Pseudomonas aeruginosa: NCIMB 10421), (Klebsiella aerogenes: NCTC 5055); and fungal strains (Candida albicans: NCPF 3179, Aspergillus fumigatus: NCPF 7097), Trichophyton tonsurans: NCPF 995). The structure of the isolated compound was elucidated on the basis of spectral data using13C-NMR, 1H-NMR and mass spectroscopy.[...] Read More.
Keywords: Commiphora glandulosa resins, antibacterial activity, novel pentacyclic triterpene, Botswana medicinal plants, antibacterial activity.
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S. J. Ibadullayeva, S. E. Mamedova, Z. R. Sultanova, N. V. Movsumova and I. A. Jafarli
Abstract: During investigations conducted within two years (2007 - 2009) 30 natural cenopopulations that were found, confirmed medicinal plants that can be used in the treatment of pediatric diseases. It was systematically studied life forms and distribution of 50 families, 94 genera and 97 species of medicinal plants widely spread in Azerbaijan flora which are used in the treatment of pediatric diseases. It was determined that the majority of medicinal plants contains grasses (71%, 69 species). Among them, perennials are characterized with the highest number (48 species, 49.5%), annuals with medium (12 species, 12.4%) and biennials with the least number (9 species, 9.3%). Profoundly described plant resources and role in the medicine of species such as, Berberis vulgaris, Crataegus pentagyna, Althaea officinalis, Hippophae rhamnoides, Inula helenium, Vaccinium myrtillis and Vaccinium vitis idaea detected and confirmed the area of distribution of medicinal plants, their vegetation phase and collected parts with priorities used in the treatment of pediatric diseases.[...] Read More.
Keywords: Medical plants of Azerbaijan flora, systematic analysis, plant resources, pharmacological effect.
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文章
G. Bedi*, Z. F. Tonzibo, K. R. Oussou, C. Choppard, J. P. Mahy and T. Y. N’Guessan
Abstract: Aqueous extracts of Chromoleana odorata are commonly used in traditional medicine as anti inflammatory drug against pains or as cataplasm to stop hemorrhage in Ivory Coast. In this work, we want to test the volatiles as essential oil extracted from the fresh leaves of C. odorata, on the cyclooxygenase function of prostaglandin-H synthase (PGHS), a protein implicated in the inflammatory process. Our goal is to make up the in vivo therapeutic activity and understand the mechanism of its biologic activity. This essential oil is extracted from leaves of C. odorata by hydrodistillation to be submitted to in vitro test. The chemical constituents of the essential oil of C. odorata increase the activity of cyclooxygenase function of PGHS with an AC 50 value of 87.5 µg/mL. Studies are in progress to indentify all the constituents implicated in the activity.[...] Read More.
Keywords: Chromoleana odorata, essential oil, cyclooxygenase, PGHS.
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文章
S. I. Ngulde*, S. Sanni, U. K. Sandabe and D. Sani
Abstract: The phytochemical constituents and antimicrobial properties of the aqueous extract of Cassia arereh stem-bark were evaluated. The extract was subjected to qualitative chemical screening of active chemical constituents and disc diffusion method was performed to determine the antimicrobial properties. The results revealed the presence of tannins, phlobatannins, carbohydrate, saponin, flvonoids, terpenes and steroids while anthraquinones, alkaloids and cardiac glycosides were not detected. The extracts (100, 200, 400 and 800 mg/ml) inhibited the growth of Staphylococcus aureus, Bacillus subtilis, Salmonella typhi, Pseudomonas aerogenosa and Enterobacter species. However, Escherichia coli, Klebsiella pneumoniae, Corynebacterium pyogenes and Candida albicans were resistant. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values were 50 mg/ml for S. aureus and B. subtilis, and 100 mg/ml for P. aeurogenosa. It was concluded that the aqueous extract of C. arereh stem-bark contained active principles having antibacterial properties and thus support the folkloric use of the plant.[...] Read More.
Keywords: Phytochemical, aqueous extract, antimicrobial, Cassia arereh.
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文章
David Iluz, *, Miri Hoffman, Nechama Gilboa-Garber and Zohar Amar
Abstract: Commiphora gileadensis is a plant that was cultivated in ancient times in the land of Israel, particularly in the oases of the Dead Sea Basin — Ein Gedi and Jericho. The plant, also known as balsam, was renowned for the expensive perfume that was produced from it, as well as for exceptional medicinal properties that were attributed to its sap, wood, bark, and seeds. This article presents the historical sources describing these health-related properties and preliminary laboratory studies demonstrating the pharmacological effects of balsam sap. Investigations of the antimicrobial activity of C. gileadensis showed the plant sap inhibitory effect against Bacillus cereus and the blocking of Pseudomonas aeruginosa lectins. These results corroborate the historical sources crediting the usefulness of balsam sap as an antiseptic agent.[...] Read More.
Keywords: Commiphora gileadensis, balsam, resin, perfume, antimicrobial effects.
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文章
Full Length Research Paper
Abstract: Uropathogenic ESCHERICHIA COLI are the most common cause of urinary tract infections, and quinolones-resistant strains cause growing concern in developing countries. This study provides relevant data in relation to the molecular epidemiology of these isolates with respect to the distribution of integron among them and in doing so, to control the infections and adopt efficient strategies. This study was performed on 96 strains of E. COLI isolated from patients with community acquired urinary tract infections in Jahrom, Iran. Having determined the antibiotic susceptibility patterns, isolates were resistant to quinolones (Ciprofloxacin, Norfloxacin and Nalidixic acid) screened for integron classes by polymerase chain reaction (PCR). Then the genetic patterns of the strains were compared by pulsed field gel electrophoresis. Of the 96 samples, 30 were resistant to at least one of the above mentioned antibiotics. Integrons were detected in 20.8, 25 and 25% of the Nalidixic acid, Ciprofloxacin and Norfloxacin resistant isolates, respectively. By using pulsed field gel electrophoresis among the 30 samples, we obtained 30 genetic patterns. The patterns revealed no clonal relationships between the strains. The gene cassettes observed in class 1 and 2 integrons did not correlate completely with the quinolone resistance observed in these isolates.[...] Read More.
Keywords: Quinolones-resistant Uropathogenic Escherichia coli, pulsed field gel electrophoresis, integrons.
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文章
B. Iromidayo Olu-Owolabi, T. Adeniyi Afolabi* and Kayode O. Adebowale
Abstract: The effect of heat moisture treatment on the functional and tabletting properties of corn starch BP was investigated. Corn starch was hydrothermally modified by heat moisture treatment (HMT) at 100°C, 16 h; 20% moisture level (HIC – 20), 25% moisture level (HIC – 25), and 30% moisture level (HIC – 30). The tablet formation properties were investigated using Heckel and Kawakita equation, while its tabletting properties were assessed using tensile strength, crushing strength, friability and disintegration time. The results reveal that HMT reduces the gelatinisation temperature and pasting properties of the corn starch. However, all the starches swell as the temperature is increased, HMT of the corn starch lowered the swelling power of the starch, while the solubility profile increased. The X-ray diffractometry study reveal that the native and HMT starches maintained the characteristic ‘A’ diffraction pattern of the cereal starch with strong peaks at 15.35, 17.15, 18.2 and 23.55 Å. The bulk density of the starch was in the order: HIC–30 ≈ HIC–25 > HIC–20 > NaIC, with reduction in the Carr’s index, compressibility index, and Hausner’s ratio after HMT treatment. The mean yield pressure, Py, (from Heckel analysis) which is inversely related to the ability of the materials to deform plastically under pressure, was relatively high after HMT of the corn starch. Also, the Pk values (from Kawakita analysis), which is related to the deformability of the individual powder increased after HMT of the corn starch compacts in the following order: NaIC[...] Read More.
Keywords: Heat moisture treatment, corn starch, tabletting properties, functional properties.
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文章
David Miruka Onyango*, Rose Kakai, Waindi Eliud Nyandago, B. Ghebremedhin, W. Konig and B. Kong
Abstract: Integron-mediated antibiotic resistance is common among diverse Salmonella serovars. Phenotypic antibiotic resistance was correlated with the presence of intergron–mediated genes in 65 Salmonella typhimurium isolates using conventional microbiological methods and PCR gene amplification. The resistance genes detected in the 20 S. typhimurium isolates were mainly those identified as part of the chromosomal multiresistance cluster balPSE-1, blaTEM and aadA. The plasmid encoded sul 1/ 2 and aphA - 1, aac (6) -1 located on the chromosome were not observed, however strB and Grm genes were observed. Genes coding for PSE -1 was predominant in the isolates as compared to TEM. The two genes were distributed differently between the various resistance phenotypes as could be observed of variability in int gene. Pathogenicity also varied in relation to individual isolate antimicrobial mechanism profile. These findings demonstrate integron mechanism as a means of antibiotic resistance in the isolates.[...] Read More.
Keywords: Intergron–mediated, Salmonella typhimurium, multiresistance, pathogenicity.
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