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Masoud Alirezaei*, Mehdi Saeb, Katayoun Javidnia, Saeed Nazifi, Najmeh Khalighyan, and Saeedeh Saeb
Abstract: Hyperhomocysteinemia is a hypothesis for the association of homocysteine with cerebrovascular diseases, neurodegenerative diseases and depression of mood. Thus, we examined whether oral betaine can act as a preventive agent in ethanol-induced hyperhomocysteinemia on the monoaminergic system. A total of 32 New Zealand White rabbits were divided into four groups (n=8) among which is the control group (C) . The ethanol group (E) was administered ethanol at a dosage of 4 g/kg daily. The betaine group (B) received betaine at a dosage 1.5% (w/w) of the diet daily, and the betaine and ethanol group (B and E) was administered with the betaine group diet; after one hour the rabbits received ethanol at a dosage of 4 g/kg daily. Blood samples were taken in the morning of the day before beginning treatment (0.0 day) and on the 30th, 60th and 90th day of the treatment. Serum folate and vitamin B12 levels were determined using a radioimmunoassay, total plasma homocysteine (tHcy) level was determined by homocysteine EIA kit, and 5- hydroxyindoleacetic acid (5-HIAA) of plasma was measured with HPLC-ECD. There was a significant negative correlation between 5-HIAA and tHcy in the E group (r=-0.473, P=0.02), and compared to the E group the concentrations of 5-HIAA in the B and E group increased considerably (p[...] Read More.
Keywords: Betaine, hyperhomocysteinemia, 5-HIAA, ethanol, vitamin B12, folate.
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文章
Nasar Yousuf Alwahaibi*, Siti Belkis Budin and Jamaludin Mohamed
Abstract: Despite the success of experimental, clinical and epidemiological studies on selenium as an anti-cancer agent, basic studies on the effects of selenium are still scanty. This study was designed to investigate the biochemical effects of sodium selenite using preventive and therapeutic approaches on chemically induced hepatocarcinogenesis in rats. Rats were divided randomly into 6 groups: negative control, positive control [Diethyl nitrosamine (DEN) + 2-Acetylaminofluorene (2-AAF)], preventive group, preventive control group (respective control for preventive group), therapeutic group and therapeutic control group (respective control for therapeutic group). The activities of plasma alanine transaminase (ALT), aspartate aminotransferase (AST), gamma- glutamyl transferase (GGT), alkaline phosphatase ALP and concentrations of total protein, albumin and globulin were determined by an auto-analyzer. GGT and ALT activities were significantly higher in the positive control, preventive and therapeutic groups when compared with the negative control. Globulin concentration was significantly lower in the positive and therapeutic group controls and higher in the therapeutic group and its respective control when compared with the negative and positive controls, respectively. Plasma GGT enzyme marker could be used as an early marker for liver neoplasm in rats. The effect of selenium on globulin, as an indicator of immunity status, needs to be clarified.[...] Read More.
Keywords: Alanine transaminase, gamma-glutamyltransferase, liver neoplasm, selenium.
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文章
K. Yaqin* and P. D. Hansen
Abstract: The aim of the study was to investigate the effects of organophosphorous pesticide on the cholinesterase activity of different organs of Mytilus edulis. The mussels were exposed to serial dilutions of the pesticides (0, 50, 100, 200, 500, and 1000 µg/l) for 96 h. A significant inhibition of the cholinesterase activity from gill occurred at the lowest concentration, which indicated that gill was the most sensitive organ. The moderate sensitive organs were foot and mantle, which were inhibited by trichlorfon at 200 µg/l. The least sensitive organs were hemolymph, posterior adductor muscle and digestive gland were inhibited at 1000 µg/l. After incubation of the trichlorfon-exposed mussels for seven days in clean media, the cholinesterase activities from different organs of mussels were not cured completely. The cholinesterase activities from hemolymph, gill, posterior adductor muscle and digestive gland recovered, while the persistent inhibition of cholinesterase activity from foot and mantle were observed.[...] Read More.
Keywords: Biomarker, cholinesterase, mussel, trichlorfon.
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文章
I. Onyesom*, R. C. Ekeanyanwu and N. Achuka
Abstract: The levels of antioxidant vitamins were estimated in Plasmodium falciparum malarial infected children aged 0 to 12 years. 113 children with P. falciparum infection were selected based on laboratory evidence and clinical symptoms. 87 apparently healthy children with no malarial parasitaemia were included as the control subjects. P. falciparum parasitaemia and serum levels of antioxidant vitamins (A, C and E) were determined using standard procedures. The results obtained showed that the mean malarial parasitaemia was 6203.01 ± 1216.79/µl and the mean serum antioxidant vitamin concentrations were 23.23±8.40 µl/dl for vitamin A, 0.49±0.18 mg/dL for vitamin C and 0.78±0.32mg/dL for vitamin E for the P. falciparum malarial infected children. The control children had higher concentrations of vitamins A (51.80±12.41 mg/dL, X2 = 60.713, P0.05) and E (0.96±0.21 mg/dl, X2 =0.039, P>0.05). The degree of malarial parasitaemia and serum concentration of vitamin E were positively correlated (r=0.42) but vitamins A (r= -0.05) and C (-0.06) were negatively correlated. Children within 0-5 years of age had higher malarial parasitaemia (7379.82± 918.99/µl), and these children had lower concentrations of vitamins A (21.27±8.68 µg/dL) and C (0.45±0.19 mg/dL) when compared with children between 6 to 12 years (vitamin A= 25.19±8.12 µg/dl and vitamin C = 0.53±0.16 mg/dL). Results suggest that the degree of malarial parasitaemia in especially children between 0 to 5 years could compromise immunity (as judged by the correlation with and reduction in vitamin E). Malarial infection among children (0 to 12 years) decreased the serum antioxidant vitamin levels, and this could lower free radical defense and contribute to the morbidity and mortality of malaria among children in this region. Health care providers should recognize these effects in planning malarial treatment and control programmes. Changes in serum antioxidant levels during post-treatment period should be investigated and documented.[...] Read More.
Keywords: Plasmodium falciparum, malaria, antioxidant vitamins, Owerri, fever.
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文章
Full Length Research Paper
Abstract: Mice infected with ESCHERICHIA COLI were protected against lethal peritonitis by the intra peritoneal administration 0.2 ml of 10 mg of poly-1,3,1,6-glucotriosyl-1,3,1,3-glucopyranose (PGG) glucan per gram body weight of animal 24 h prior to bacterial infection. This procedure employed in this study, is similar to that employ in a previous studies where rat model were protected with β-glucan against intra-abdominal sepsis, where the intramuscular doses of 10 ml to 10 g per animal 24 h and 4 h prior to surgical implantation of the bacterial inoculums reduced the early mortality associated with the peritonitis phase of this experimental disease process. In this study mice were protected with β-glucan against peritonist 24 h prior to intraperitoneal administration of bacteria inoculums. Quantitative cultures of the liver obtained from mice infected with E.COLI showed significantly the liver of PGG glucan-treated infected animal has a reduced microbial load as compare to those infected and were not treated. The transient increase in survival rate of mice infected with E.COLI that have been treated with PGG glucan- as compare with that of those not treated shows the significant effect of β glucan on E.COLI infection.[...] Read More.
Keywords: Beta-glucan, Escherichia coli, poly-131-6-glucotriosyl-j31-3-glucopyranose (PGG) glucan, peritonitis, inoculums, intra-peritoneal, intramuscular
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S. Umesha* and R. Kavitha
Abstract: Cinnamyl alcohol dehydrogenase (CAD) is known to be involved in the defense related responses in many host-pathogen systems. Induction of CAD enzyme in bacterial spot disease resistance in tomato (Solanum lycopersicum Mill.) was investigated in the present studies. Twenty different tomato cultivars were screened for resistance against bacterial spot disease incited by Xanthomonas axonopodis pv. vesicatoria under field conditions. Field screening was performed by artificially inoculating X. axonopodis pv. vesicatoria suspension to four-week-old tomato seedlings and observed for the typical symptoms of bacterial spot disease. They were categorized into highly resistant, resistant, susceptible and highly susceptible cultivars on the basis of disease incidence under field conditions. Tomato cultivars were subjected for estimating CAD - a defense-related enzyme. Temporal pattern of the enzyme was estimated using four cultivars representing each category of tomato cultivars by inoculating with six isolates of X. a. vesicatoria. Native PAGE analysis of CAD was carried out for the time course of enzyme activity and also by selecting three different tomato cultivars, after infecting with the pathogen. Based on the inducible amounts of the enzyme upon pathogen infection, the tomato cultivars were correlated with that of disease incidence under field conditions. A significant (P≤0.05) correlation was observed between the degree of host resistance and the enzyme level. In highly resistant tomato cultivar the enzymatic level was increased in comparison with highly susceptible tomato cultivars. Isoform analysis of CAD enzyme indicated a clear difference between the number of isoforms and also the intensity of each isoform after pathogen infection with the resistant and susceptible tomato cultivars. A possible regulation of CAD in imparting host resistance is discussed here.[...] Read More.
Keywords: Tomato, Solanum lycopersicum, bacterial spot, Xanthomonas axonopodis pv. vesicatoria, cinnamyl alcohol dehydrogenase, resistance, susceptibility.
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Mohammad Shahriar* and Nishat Zareen Khair
Abstract: According to the latest data from the Centers for Disease Control and Prevention (CDC), the six ESKAPE bacteria are responsible for two thirds of all health care-associated infections (HAIs) including Escherichia coli. Klebsiella species and their gram-negative cousin E. coli together accounted for 18 percent of all HAIs in 2006 to 2007, and a growing proportion of these two bad bugs carry resistance to a remarkable spectrum of antibiotics. Of the very few drugs in late-stage development, none works by a novel mechanism. The aim of our study is to correlate the plasmids with drug resistance of clinical isolates of E. coli. Twenty two clinical isolates of E. coli were collected from different diagnostic centers of Dhaka and their antimicrobial susceptibility pattern was tested. Seven multidrug resistant isolates of E. coli were selected and their antibiotic susceptibility pattern was tested before and after curing. Our study revealed that 100% of the isolates (22 isolates) were resistant to cephalexin, cephradine, oxacillin, penicillin and vancomycin. 95.45% of the isolates were resistant to ciprofloxacin, cloxacillin and imipenem. 90.91% isolates (21 isolates) were resistant to erythromycin. 81% of the isolates (18 isolates) were resistant to amoxicillin. 72.72% (16 isolates) were resistant to co-trimoxazole, 63.64% (14 isolates) were resistant to tetracycline. 31.81% (7 isolates) were resistant to ceftriaxone and neomycin. 22.73% (5 isolates) were resistant to gentamicin and only 13.64% of the isolates (3 isolates) were resistant to chloramphenicol. There was no significant difference in the antibiotic susceptibility pattern before and after curing indicating no correlation between plasmid and drug resistance in the 7 isolates of E. coli. However, isolate number 5 which was resistant to gentamicin, neomycin and imipenem became sensitive after curing. Similarly sample number 1 became sensitive to rifampicin and imipenem and sample number 7 became sensitive to imipenem after curing.[...] Read More.
Keywords: Multi-drug resistant, Escherichia coli, antimicrobial susceptibility, plasmid, curing.
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K. Thirumalai Raj, G. Jeyasekaran*, R. Jeya Shakila, A. Jemila Thangarani and D. Sukumar
Abstract: A rapid and sensitive multiplex polymerase chain reaction (MPCR) based assay was developed for the detection of Salmonella enterica serovars such as Typhi (ATCC 122235), Paratyphi A (MTCC 735), Typhimurium (MTCC 98), Enteritidis (ATCC 13065), Weltevreden (MTCC 1169) Bovismorbificians (MTCC 1162), Brunei (MTCC 1168), Arizonae (MTCC 660) and Infantis (MTCC 1167) in shrimps within 4 h of pre-enrichment. The Salmonella genus specific gene of himA gene was selected and 16S-23S internal transcribed spacer region was used as an internal amplification control (IAC). The genomic DNA was extracted by using boiling and centrifugation method. Sensitivity of the assay was tested by artificially inoculating the shrimp homogenate with viable cells of Salmonella. The MPCR assay could detect up to 5 cells within 4 h of pre-enrichment. Amplification of DNA extracted from other bacterial pathogens viz. Vibrio cholerae (NICED 16582), Escherichia coli (ATCC 9637) and Staphylococcus aureus (ATCC 12598) yielded negative results. This MPCR assay provides specific, rapid and reliable results and allows for the cost effective detection of serovars of S. enterica in one reaction tube in mixed bacterial communities that are prevalent in shrimp products.[...] Read More.
Keywords: Multiplex polymerase chain reaction (MPCR), Salmonella enteric, himA, 16S-23S spacer region, 4 h assay.
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Tyagi Shruti, Tyagi Pankaj K.*, Panday Chandra Shekhar and Kumar Ruchica
Abstract: A randomized sampling from open air of the kitchens in rural vs urban households to determine bacterial contamination of Haryana (India) were carried out by taking 80 samples between July to September 2009. 40 samples of each in rural and urban area were collected in culture plates. The inoculation procedures were varied from direct inoculation of the kitchen air into the nutrient agar medium. Identification by bacterial taxonomy key, different morphological and biochemical tests in rural households, numbers of bacteria revealed Salmonella spp., Acinetobacter spp., Pseudomonas spp. and Paenibacillus spp. with 9 different strains and in urban households, numbers of bacteria revealed Bacillus spp., Pseudomonas spp., Micrococcus spp., Paenibacillus spp. and Acinetobacter spp. with 27 strains. Among the isolates, Salmonella spp. (80%) followed by Acinetobacter (63%), Pseudomonas putida (38%) and Paenibacillus polymyxa (30%) were observed in rural areas. In urban areas Bacillus spp. (88%), Pseudomonas spp. (75%), Micrococcus spp. (70%), Paenibacillus spp. (38%) and Acinetobacter spp. (30%) were observed. The bacteriological quality of air of kitchens in rural households was found to be more pathogenic and virulent as compared to that of kitchen in urban households. These opportunistic pathogens may be harmful, especially in immunocompromised host. In this setting, there is a constant risk of contamination and transfer to willing host. Hence, better quality of air can be achieved by manipulating sanitation and hygiene within houses, kitchens and surrounding areas.[...] Read More.
Keywords: Air of kitchens, households, bacteriological quality, sanitation and hygiene.
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Qurban Ali*, Muhammad Hammad Nadeem Tahir , Hafeez Ahmad Sadaqat, Saeed Arshad, Jahenzeb Farooq, Muhammad Ahsan, Muhammad Waseem and Amjad Iqbal
Abstract: The present studies were conducted to the estimation of correlation for quantitative traits in chickpea (Cicer arietinum L.) in the field of the department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, during the crop season 2009 to 2010. Correlation studies showed that biomass per plant, number of pods per plant, number of secondary branches per plant, number of seeds per pod and 100-seed weight were positive and significant at genotypic level but positive and highly significant at phenotypic level. Whereas number of days taken to flowering, number of days taken to maturity, primary branches per plant, secondary branches per plant were positively correlated with the grain yield per plant at genotypic and phenotypic levels. Plant height was negative and non-significantly correlated with grain yield per plant at both genotypic and phenotypic levels.[...] Read More.
Keywords: Cicer arietinum, correlation, genotypic, phenotypic, chickpea, Pakistan.
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