Advanced Journal of Microbiology Research

Abstract: Drinking water systems supplied by untreated groundwater were examined to determine whether coliform or heterotrophic plate count bacteria are capable of growing. Filterable bacteria were present in 42% of the 46 groundwater sources examined by using nonselective media (R2A and full strength m-HPC agars). Pseudomonads were the most frequently identified group of filterable bacteria detected. Flavobacterium, Alcaligenes, Acinetobacter and Achromobacter isolates were also identified. Total coliforms were recovered on…

Abstract: The bacterial isolate HJ1, which was identified as a Methylobacterium sp., grew on 2, 2-dichloropropionic acid as the sole carbon source and produced a 2-haloalkanoic acid hydrolytic dehalogenase. This non-stereospecific dehalogenase E (DehE) catalysed the hydrolytic dechlorination of 2, 2-dichloropropionic acid and D, L-2-chloropropionic acid to produce pyruvate and lactate, respectively. The enzyme was purified to homogeneity and characterized. The molecular weight was 36 kDa by SDS-polyacrylamide gel electrophor…

Abstract: Cassava granules coated with Newcastle disease virus (NDV) strain V4-UPM was used to vaccinate free-range chickens in their natural habitat. Immune response, vaccine virus excretion and the efficacy of the food vaccine were assessed by standard methods. Results show that out of 218 chickens given initial food vaccine in the four locations, 138 (63.3%) produced detectable HI antibody while 202 (92.7%) had titres < 3.0. However, only 16 (7.3%) attained log2 3.0 with GMT of 3.2. This was made up of Nchara- Akanu 7(12…

Abstract: Three essential oils, namely thyme, marjoram and sage, were added to concentrated yoghurt (labneh) at concentrations of 0.2, 0.5 and 1.0 parts per million (ppm). Subsequently, the chemical, microbiological and organoleptic properties of freshly prepared labneh and of the labneh stored at 5˚C ± 1 for up to 21 days were determined. Addition of essential oils affected the pH, soluble nitrogen -to-total nitrogen, total volatile fatty acid and acetaldehyde values of the prepared labneh. On the other hand, total solids a…

Abstract: A new nematode extraction technique was established, which is based on an iso-osmotic density- gradient medium (OptiPrepTM). This technique resulted in significantly higher numbers of clean eggs and vermiform nematodes that retain higher viability (48.6%) than samples processed with the sucrose method (28.7%). Nematodes survived exposure to OptiPrepTM for 22 hours without significant mortality whereas all nematodes died in the sucrose medium. OptiPrepTM provided a suitable, non-toxic alternative to the traditional…

Abstract: Petroleum ether, chloroform (CHCl3), ethanol (EtOH), methanol (MeOH) and aqueous (H2O) extracts of leaves of Asperugo procumbens L. were evaluated for antibacterial activity against five human pathogenic bacterial strains with the agar-well diffusion method. The methanol extract was highly active against all the test bacteria, followed by the ethanol and aqueous extracts. The chloroform and petroleum ether extracts did not show any antibacterial activity. The minimum inhibitory concentration (MIC) of the methanol e…

Abstract: Difficulties in the management of dengue infections include the lack of rapid diagnostic methods and symptoms of dengue are often confused with those of other diseases. Five commercial kits were evaluated for the detection of dengue-specific IgM and IgG antibodies using sera obtained from patients with primary and secondary dengue infections, as well as other febrile illnesses. All kits were compared to the in-house IgM ELISA and HI assays. The rapid test kits took either 30 to 45 min (PanBio Dengue Duo Cassette, A…

Abstract: Water hyacinth is a promising plant for the production of bioethanol. The water hyacinth was saccharified with diluted sulfuric acid and fermented to ethanol by yeast obtained from different hydrospheres. We found that the best conditions for water hyacinth hydrolysis were 1% (v/v) sulfuric acid at 121oC for 1 h. In the next step, we searched for yeast that could produce ethanol from the water hyacinth hydrolysate. For this, 624 strains were isolated from 28 bodies of water. Strain 484 produced 22.4 ml of ethanol/k…

Abstract: A trial of a screening and selection strategy for phosphate-solubilizing bacteria based on phosphate solubilization ability, and the subsequent effect of these strains on plant growth promotion under in situ conditions was conducted. Of all the bacteria tested, three Pseudomonas fluorescens strains (CB501, CD511 and CE509) were selected. On agar plates, two strains (CB501 and CE509) showed an ability to solubilize the three phosphate types (Ca3 (PO4)2, AlPO 4·H2O or FePO4·2H2O), while strain CD511 showed a halo zon…

Abstract: Advanced Journal of Microbiology Research Vol. 2012 Available online at http://internationalscholarsjournals.org/journal/ajmr © 2012 International Scholars Journals Full Length Research Paper Statistical optimization of medium components for chromate reduction by halophilic Streptomyces sp. MS-2 Mona E. M. Mabrouk Botany Department, Faculty of Science, Damanhour, Alexandria University, Egypt. E-mail: mona_mabrouk_eg@hotmail.com. Accepted 18 March, 2012 Extensive use of hexavalent chromium Cr(VI) in various industri…

Abstract: This study investigated and compared the microbiological quality of source, transported and stored water in Lungwena households. It also examined water management practices at all the investigated points. One hundred and eighty (180) water samples were collected from 6 villages and tested for Escherichia coli, Salmonella, E .coli 0157:H7 and Campylobacter jejuni using standard methods. Water contamination practices were observed in two hundred and eighty seven households. E. coli, Salmonella, E. coli 0157:H7 and C…

Abstract: Six different organic solvents such as ethanol, methanol, petroleum ether, hexane, benzene and chloroform were used to extract the bioactive compounds from the rhizome of Drynaria quercifolia to screen the antibacterial activity against infectious disease causing bacterial pathogens such as Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella typhi, Salmonella paratyphi A, Salmonella paratyphi B, Salmonella marscence, Staphylococcus aureus and Bacillus subtilis by agar diff…

Abstract: The prevalence of lactic acid bacteria (LAB) in Senegalese local food products was determined to be 109 CFU/g in millet flour and milk products, and 103 CFU/g in seafood products. These food products are generally preserved by spontaneous fermentation (without addition of starters). Of 220 lactic acid bacteria strains randomly selected from such products, 12 isolates capable of producing bacteriocin-like substances (bac+) were detected. Based on the use of API 50 CH test kits and 16S rDNA sequencing, 11 isolates we…

Abstract: The essential oil obtained by hydrodistillation from the resin of Dacryodes edulis (G. Don) H. J. Lam was simultaneously analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC -MS). Twenty four components were identified in the essential oil and the main components were sabinene (21.8%), terpinene-4-ol (19.8%), -pinene (17.5%) and p-cymene (11.3%), respectively. The antioxidant capacity of the essential oil was examined using an in vitro radical scavenging activity test and -carotene-lino…