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Advanced Journal of Microbiology Research

15 Articles | Volume 23 (2024)
Review
Halit Özkaya, Abdullah Bariş Akcan* and Gökhan Aydemir
Abstract: Mastoiditis is an infection of mastoid process, the portion of the temporal bone of the skull which is behind the ear and contains open air-containing spaces. With possible extra cranial and intracranial complications, acute mastoiditis is the leading complication of acute otitis media (AOM). The goal of this review was to assess the clinical features, pathogens, complications and management of acute and chronic mastoiditis. Retrospective systematic review of studies and articles on acute and chronic mastoiditis between 1983 and 2010 in pubmed, include clinical, epidemiological, microbiological, treatment and outcome data. In 10 studies, the average age was 16, ranging from 6 months to 70 years, with 55% from 0 to 5 years. Most common symptom was otalgia (84 %), 58% of patients had history of past AOM and 61% were under antibiotic therapy during admission. 74% presented with retroauricular swelling and erythema, 58% had a displaced pinna. In general, the prevalence of organisms causing mastoiditis varies greatly between studies, among countries and according to the age of the patient. Reported pathogens were as follows: Streptococcus pneumoniae, most frequently isolated pathogen in acute mastoiditis, prevalence of approximately 25% Group A beta-hemolytic streptococci, Staphylococcus aureus, Moraxella catarrhalis, Haemophilus influenzae, Pseudomonas aeruginosa, Mycobacterium species, Aspergillus fumigatus and other fungi, Nocardia asteroides. Extension of the infectious process beyond the mastoid system can lead to a variety of intracranial and extracranial complications, including meningitis, cerebral abscess, epidural, subdural and intraparenchymal abscesses, vascular thrombosis, osteomyelitis, and abscesses deep within the neck. Permanent damage of the ear leads to hearing loss, vertigo and sometimes facial weakness. The Cochrane review found that antibiotic treatment of otitis media may play an important role in reducing the risk of mastoiditis in populations where it is more common. Despite the use of antibiotics, acute mastoiditis still remains a threat to patients with AOM, especially children under 5 years of age. On the basis of the clinical features and imaging findings, the disease is managed conservatively with intravenously administered antibiotics or treated with mastoidectomy and drainage plus antibiotic therapy. Great care is required by clinicians to make an early diagnosis in order to promote adequate management and prevent complications.[...] Read More.
Keywords: Mastoiditis, otitis media, childhood.
  2024, 23(1), 1-6; 
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Review
Freitas, A. C.*, Silva M. A. R., Jesus, A. L. S., Mariz, F. C., Cordeiro, M. N., Albuquerque, B. M. F. and Batista, M. V. A
Abstract: Bovine papillomaviruses (BPV) are double-stranded DNA viruses that infect the cutaneous and mucosal epithelia inducing hyperplasic lesions in cattle. BPV is the etiologic agent of the papillomatosis and neoplasia of the upper gastrointestinal tract and urinary bladder. The benign and malignant tumors caused by BPV are emergent diseases important for beef and dairy cattle in the world. Although BPV associated tumors have veterinary and agricultural relevance, they have also been studied as a relevant model of human papillomavirus (HPV). Recent studies in BPV biology have shown a great diversity of BPV types and new putative BPV types infecting and co-infecting the herd in several parts of the world. This review will briefly summarize the genomes and structure of BPV and the bovine papillomatosis; will describe in greater detail the genotypic diversity, BPV cross-species infection, relevant aspects of BPV and co-infection and its possible routes of transmission. These new approaches about BPV may be very useful to understand the oncogenic potential of the virus, the relationship between virus and co-factors, and the development of anti-viral vaccines.[...] Read More.
Keywords: Bovine papillomavirus, co-infection, virus transmission, BPV diversity.
  2024, 23(1), 1-6; 
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Research Article
R. Muthezhilan, R. Ashok and S. Jayalakshmi*
Abstract: The objectives of the present study were Isolation, identification and characterization of xylanase producing fungi, optimization of medium composition and cultural conditions for xylanase enzyme production, production using cheaper sources and extraction and partial purification of extra cellular xylanase enzyme from a potential strain. Xylan has a complex structure consisting of -1, 4-linked xylose residues in the backbone to which short side chains of o- acetyl, -L-arabinofuranosyl, D - - glucuronic and phenolic acid residues are attached. A variety of microorganisms are reported to produce endo xylanases, that can degrade -1,4-xylan in a random fashion, yielding a series of linear and branched oligosaccharide fragments. Totally 69 strains were isolated from Pitchavaram mangroves. In secondary screening, based on the diameter of the clear zone formation in oat spelt xylan agar plates, Penicillium oxalicum was selected and optimized for xylanase enzyme production in solid state fermentation using cheaper sources like wheat bran, rice bran, rice straw, sesame oil cake and wood husk. Maximum enzyme activity was observed in wheat bran. (3.89 U/ml) Optimum pH and temperature for xylanase activity were found to be 8 and 45°C at 3% salt concentration. In purification step, 80% ammonium sulphate saturation was found to be suitable giving maximum xylanase activity. The use of wheat bran as a major carbon source is particularly valuable because oat spelt xylan or birch wood xylan are more expensive, Thus the present study proved that the fungal strain P. oxalicum used is highly potential and useful for industrial production.[...] Read More.
Keywords: Xylanases, solid state fermentation, fungi, optimization, purification.
  2024, 23(1), 1-6; 
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Research Article
Abdoulaye Seck, Abdou Diop, Babacar Ndiaye, Chantal Mahou Douala-Djemba, Thierno Abdoulaye Diallo.
Abstract: Background: Neisseria gonorrhoeae, causative agent of gonorrhea, has emerged in recent years by acquiring resistance to antimicrobials recommended for treatment. The aim of this study was to determine antibiotic resistance profile of N. gonorrhoeae strains isolated in Dakar. Methods: This retrospective study involved strains of N. gonorrhoeae isolated from pathological samples of patients received at medical biology laboratory of Pasteur Institute in Dakar. Samples were inoculated on Chocolat PVX and Chocolate+VCN agar plates incubated at 37°C under 5% CO2 for 24-48 hours. Species identification was performed using Api-NHÒ identification gallery. Minimum inhibitory concentration was determined by E-test method with 0.5 McFarland bacterial suspension prepared from few colonies of pure culture with NaCl 0.85% Medium according to EUCAST 2022 criteria. Results: 41 strains of N. gonorrhoeae were isolated from patients (40 men and one woman). Median age was 38 years and age group most represented was [20-29 years] with 36.6%. Isolates were sensitive to ceftriaxone, cefixime and gentamycin (100%) and to azyhtromycin (95.1%).  However, they were resistant to tetracycline (92.7%) and ciprofloxacin (95.1%). Conclusion: This study revealed third-generation cephalosporins (ceftriaxone and cefixime), gentamicin and azyhtromycin are still effective, and are drugs of choice in current recommendations for treatment of gonorrhea.[...] Read More.
Keywords: Neisseria gonorrhoeae, Sexually transmitted infection, Antimicrobial resistance, MIC, Senegal.
  2024, 23(1), 1-6; 
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Research Article
Aiyegoro O. A.*, Igbinosa O. O., Ogunmwonyi I. N., Odjadjare E. E., Igbinosa O. E. and Okoh A. I.
Abstract: This study was carried out in order to determine the incidence of urinary tract infection in children and adolescents, identify the uro-pathogens responsible for the infection and study the antibiotic sensitivity patterns of the uro-pathogens. Clean voided mid-stream urine samples were collected in sterile universal bottles from 301 children and adolescents between the ages of 5 and 18 years at the Obafemi Awolowo University Teaching Hospital complex (OAUTHC) Ile-Ife, Nigeria from December 2005 - July 2006. Culture plates with bacteria counts greater than or equal to 1x105 cfu-ml-1 were taken as positive, thus indicative of Urinary Tract Infection (UTI). The bacteria isolates were identified based on colony morphology characteristics, Gram stain reaction and biochemical tests using API 20E kits. The identified bacteria were then tested in vitro with standard antibiotics disc to determine their antibiotics sensitivity patterns. The result of this study shows that 36 (11.96%) of the 301 patients studies had UTI. Of the 124 females examined, 28 (22.4%) had positive urine culture while 8 (4.56%) of the 177 males had significant bacteriuria. A total of 36 bacterial isolates were obtained. Escherichia coli constituted the predominant organism and was responsible for (52.77%) of the cases of UTI. This was followed by Klebsiella sp. (25%), Proteus mirabilis (13.89%), Streptococcus faecalis (5.56%) and Pseudomonas aeruginosa (2.78%). The antibiotics sensitivity test revealed a high level of resistant to cotrimoxazole, amoxicillin and colistin as more than 60% of the isolates were resistance to these. This study highlights the presence of multi-resistance P. aeruginosa and poor compliance of the pathogens in vitro to antibiotics commonly used in treating UTI. It is therefore suggested that appropriate antimicrobials be administered to reduce the risk of multiply resistance organisms developing and avert ineffectiveness of antibiotics. Prompt therapeutic intervention is also essential to prevent cases of asymptomatic UTI from becoming symptomatic with resultant damage.[...] Read More.
Keywords:
  2024, 23(1), 1-6; 
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Research Article
AKAFOU Ethien Felix*, GOLY Kouassi Roselin Cyrille, ATOBLA Koua, SORO Yaya and ADIE Adjehi
Abstract: Cocoa cultivation generates large quantities of cocoa pod shells, the management of which is a concern for producing countries. This study aims to valorize cocoa pod waste by determining the chemical composition and antifungal activities of their hydroethanolic extracts. Secondary metabolites were extracted from the dried powder of plant material by maceration in hydroalcoholic solvent. Their families were determined by phytochemical screening and certain contents determined by dosage. Antifungal activities were evaluated in solid and liquid media. Extraction yields were 14.10 ± 0.08 and 11.92 ± 1.81% respectively for green and yellow pod shells. Phytochemical screening revealed the presence of all the families sought in the yellow and green pod shells. Total polyphenol and flavonoid contents of yellow pods are higher than those of green pods with values ​​of 35.50 ± 0.50 mg EAG/g and 65.33 ± 0.33 mg EQ/g of dry extract, respectively. However, condensed and total tannins are more abundant in green pods with contents of 16.40 ± 0.02 and 23.98 ± 0.02 mg EAT/g, respectively. Antifungal tests revealed that the four fungal strains tested were more sensitive to hydroethanol extract of yellow pod husks. Results obtained show that cocoa pod waste can be used in pharmaceutical field.[...] Read More.
Keywords: Cocoa pod waste, hydro-ethanolic extracts, secondary metabolites, antifungal activity.
  2024, 23(1), 1-6; 
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Research Article
Yahaya S. M.*, Mukhtar D., Ali M. U. , Lawan M., Ibrahim A. M., Amina L. A., Tasiu A. M., Sadiya A. B., Maimuna M. D. and Musa M. J.
Abstract: Experiment was carried out to compare the susceptibility of Capsicum annuum and Capsicum frutescens on sale to fungal infection at Sharada and Rimi markets in Kano metropolitan area. A total of four fungal species were isolated from samples of C. annuum and C. frutescens collected from the two markets. Out of the colonies isolated, the most abundant colony was A. niger (84, 39.25%). This was followed by R. stolonifer (71, 33.17%) and A. fumigatus (37, 17.28). The least occurring colony was A. flavus (22, 10.28). The difference between the four species was statistically significant at P < 0.001. On the basis of location, more colonies were isolated from samples collected from Sharada market (126, 58.87%) than Rimi market (88, 41.12%). The results indicate that hot or sweet features have no influence on the fungal infection of the sample from the two locations. More isolates were counted on Mondays (133, 62.1%) while 81 (37.9%) were counted on Thursdays. The differences between Mondays and Thursdays exposure was statistically significant at P[...] Read More.
Keywords: Capsicum annuum, Capsicum frutescens, fungi, Rimi, Sharada.
  2024, 23(1), 1-6; 
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Research Article
Ndisio Boaz*, Peter Wachira, Victor Kagot and Sheila Okoth
Abstract: Groundnut is one of the staple foods in many parts of the world. Due to its high nutrient content, the nuts are liable to colonization by aflatoxigenic fungi and subsequent aflatoxin accumulation. This study was aimed at determining susceptibility of locally grown groundnut varieties to Aspergillus flavus in Homa Bay County, Western Kenya. A pretested questionnaire was used to survey agronomic practices on groundnut cultivation in 75 randomly selected households in the study site. From each household farm, 100 g soil samples and 500 g of groundnuts were collected at harvest and A. flavus isolated on Modified Rose-Bengal Agar and identified. Aflatoxin was then extracted from each of the groundnut samples and quantified using direct competitive enzyme linked immunosorbent assay (ELISA). Red Valencia was the most cultivated among the 8 varieties identified. Farmers (66%) obtained the planting seeds from the local market and most (92%) did not use fertilizers with majority (94%) having no knowledge of aflatoxins. There was no significant inter-variety difference in aflatoxin accumulation (p=0.744, F=0.581, Df=6, 61). A highly significant association (t = 2.652; P = 0.010) was found between storage state of the groundnuts and aflatoxin levels, with 94% of the samples stored unshelled having aflatoxin levels below 10 ppb. Overall, only 6.7% of kernels sampled from all the divisions did not meet the EC aflatoxin limit of ≤4 ppb while 4% did not meet the KEBS limit of ≤10 ppb. Though the agronomic practices were poor, aflatoxin levels were predominantly low in the region suggesting that the aflatoxin accumulation is likely influenced by agro-ecological zoning as other studies have also been indicated.[...] Read More.
Keywords: Groundnuts, Aflatoxins, Aspergillus flavus.
  2024, 23(1), 1-6; 
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Research Article
Doughari, J. H.*, Elmahmood, A. M. and Nggada, H. P.
Abstract: Due to reported cases of antimicrobial resistance by many pathogenic bacteria against many antibiotics worldwide, and the sparse nature of antimicrobial resistance data, a retrospective study was carried out on 744 isolates of Salmonella typhi obtained from 974 samples from four different hospitals in Yola, Adamawa State, Nigeria between 2001-2004 to determine the resistance pattern of S. typhi to the most commonly used antibiotics cotrimoxazole, ciprofloxacin, chloramphenicol and ampicillin. High rates of resistance was found in most of the isolates studied. Resistance rates were 92.3, 88.8, 79.6, 53.5 and 20% to amoxicillin, ampicillin, chloramphenicol, cotrimoxazole and ciprofloxacin, respectively. The high percentage resistance to the antibiotics studied could be attributed to their prevailing usage and abuse in the area under study. The implication of the high percentage resistance recorded for the antibiotics is that only ciprofloxacin will effectively treat S. typhi infections. These results call for nationwide surveillance programme to monitor microbial trends and antimicrobial resistance patterns in Nigeria.[...] Read More.
Keywords: Antibiotics, resistance, surveillance, Nigeria, Salmonella typhi.
  2024, 23(1), 1-6; 
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Research Article
Doughari, J. H.*, Elmahmood, A. M. and Manzara, S.
Abstract: The bioactive compounds of root extracts of Carica papaya L. were extracted, using water and organic solvents, and were investigated for antibacterial activity against some pathogenic bacteria using the cup plate agar diffusion method. The aqueous extracts did not show significant activity, but the organic extracts had significant activity with the methanol extracts demonstrating the highest activity against the test bacteria. The extracts demonstrated higher activities against all the gram-negative bacteria than the gram-positive bacteria tested, with the highest activity (14 mm zone of inhibition) demonstrated against Salmonella typhi. Increase in temperature enhanced the activity of the extracts, while alkaline pH decreased the activity. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the extracts ranged between 50-200 mg/ml. Preliminary phytochemical analyses showed that the extracts contain alkaloids, tannins, saponins, glycosides and phenols. Carica papaya may be used for the treatment of gastroenteritis, uretritis, otitis media, typhoid fever and wound infections.[...] Read More.
Keywords: Carica papaya L., antibacterial activity, phytochemical analysis, antibiotics.
  2024, 23(1), 1-6; 
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Research Article
Nur Ceyhan* and Guven Ozdemir
Abstract: A strain  of  poly- -hydroxybutyrate (PHB)-accumulating  bacterium  was isolated  and  identified  as Enterobacter aerogenes (designated E. aerogenes 12Bi) by using biochemical and phylogenetic characterization. The accumulation of a large amount of granules in its cells cultured in the domestic wastewater medium (DWWM) were showed by transmission electron microscopy (TEM). When PHB production by our strain was determined by Hypochlorite method, it was found that PHB production ranged from 16.66 to 96.25% (w/w). The highest PHB yield by our microorganism was up to 96.25% within 18 h in DWWM 5 (supplemented with 100% DWW). This is the first report of the use of DWW for production of PHB by E. aerogenes. The results obtained in the study demonstrated that PHB could be efficiently produced to a high concentration with high productivity by using DWWM as an inexpensive substrate. Thus, it can contribute to the reduction of high production cost of PHB.[...] Read More.
Keywords: Poly- -hydroxybutyrate (PHB), bioplastics, domestic wastewater, Enterobacter aerogenes, biochemical identification, phylogenetic identification.
  2024, 23(1), 1-6; 
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Research Article
Shahram Nanekarani*, Cyrus Amirinia and Nour Amirmozafari
Abstract: In this study, the genetic variation in Karakul sheep was investigated using 15 microsatellite markers (MCMA2, BMS460, BM1815, OARCP26, OARFCB20, MAF64, OARAE129, BMS332, LSCV38, BM6444, BMS995, MCMA26, BMS678 and OARCP49) and all fifteen loci were amplified successfully. Genomic DNA was extracted from 120 blood samples, using modified salting-out method. Tests of genotype frequencies for deviation from the Hardy-Weinberg equilibrium (HWE) were performed at each locus and revealed significant departure from HWE (P < 0.001) due to heterozygote excess. Parameters of variability such as effective number of alleles and gene diversities corroborated with the high level of variation frequently displayed by microsatellite markers. The fifteen tested loci were all polymorphic. Furthermore, other criteria of genetic variation including polymorphism information content (PIC) values and Shanon information index were calculated in this study. Results showed that, high level of genetic diversity was observed in Karakul breed and this breed was not at risk for conservation concept. This research has also shown that microsatellite technique is a useful tool for evaluation of genetic variation among domesticated animals.[...] Read More.
Keywords: Karakul sheep, microsatellite markers, genetic variation, polymorphism. Hardy-Weinberg equilibrium (HWE).
  2024, 23(1), 1-6; 
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Research Article
Wei Yang, Lei Ji, Li-Rong Tan, Shi-Mo Li, Yan Wang, Hong-Xia Liu*, and Yu-Ming Luo*
Abstract: One pair of primers was designed based on the sequence of tmr locus for specific and sensitive detection of Agrobacterium tumefaciens. Only the A. tumefaciens strain can produce the 236bp target fragment among the fourteen bacterial species that tested. The sensitivity of the specific PCR system was determined by a nested-PCR amplification which can numbered the copies of the template DNA. According to the results, it can give positive band when only 100 copies were in the template. The protocol was carried out for detection A. tumefaciens of twelve soil samples collected from six different gardens in Shanghai where crown gall happened. Two of the samples which collected from symptomless gardens also give the positive band. Based on the results we can make a conclusion that this pair of primers can be a useful tool in detecting A. tumefaciens, especially in detecting latent infection of this devastating pathogen.[...] Read More.
Keywords: Agrobacterium tumefaciens, detection, polymerase chain reaction (PCR).
  2024, 23(1), 1-6; 
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Research Article
Mohammad Kargar*, Maryam Baghernejad, Abbas Doosti and Sadegh Ghorbani-Dalini,
Abstract: To determine the 23S rRNA point mutations in clarithromycin resistance of Helicobacter pylori strains isolated from southwest, Iran. This was a cross-sectional survey, which was done on 263 patients who referred to endoscopy department of Shehrekord university of medical sciences. According to gram stain, urease, catalase, oxidase and polymerase chain reaction (PCR) H. pylori identified. Standard National Committee for Clinical Laboratory Standard (NCCLS) method used for assessment of clarithromycin resistance. Specific primers and restriction enzymes BsaI and MboII by PCR-RFLP were used for analysis of A2143G and A2142G mutations. So for the detection of A2142C, specific primers and PCR method were used. 84 strains of H. pylori (31.94%) determined by PCR method. Of 19 (22.62%) clarithromycin resistant strains 13 (68.40%), 3 (15.78%), 2 (10.52%) had A2143G, A2142G, A2142C respectively and one unknown mutation in 23S rRNA gene. Because of considerable resistance to clarithromycin, direct diagnosis of this mutation by molecular approach in other parts of the country is necessary.[...] Read More.
Keywords: Polymerase chain reaction, clarithromycin, resistance, 23S rRNA, polymerase chain reaction - restriction fragment length polymorphism.
  2024, 23(1), 1-6; 
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Research Article
Hang’ombe Bernard Mudenda*, Ulaya William, Mwansa James C. L., Mubita Charles, Isogai Nayuta, Mulenga Evans, Moonga Ladslav, Isogai Hiroshi and Isogai Emiko
Abstract: Pathogenesis of Salmonella depends upon a large number of factors controlled by an array of genes that synergise into actual virulence. The goal of this study was to detect Salmonella invA, spiC and sipC directly from clinical specimens, using the dot blot hybridization assay. We detected invA , spiC and sipC as a one combination from 4.5% (95% CI: 2.21 to 8.64) human feacal and 35.2% (95% CI: 26.4 to 45.0) poultry samples after enrichment. Furthermore the dot blot method had a higher sensitivity than routine culture, before and after enrichment. These results indicate that dot blot hybridization may be used to directly detect Salmonella invA, spiC and sipC in clinical samples.[...] Read More.
Keywords: Salmonella, dot-blot hybridization, spiC, sipC, invA, clinical-samples.
  2024, 23(1), 1-6; 
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