MOSP - Macao Scientific Publishers

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Isolation and partial characterization of bacteriocin produced from Lactobacillus plantarum

V. Karthikeyan* and S. W. Santosh

Abstract: Bacteriocin producing Lactobacillus plantarum strain isolated from marine shrimp (Penaeus monodon) gut, showed broad range of antibacterial activity against some major food born pathogens. Maximum bacteriocin production was observed at 50°C, pH 4 and 0.9% sodium chloride solution. The bacteriocin has purified by ammonium sulphate precipitate and ion exchange (DEAE cellulose) chromatography. Biochemically it was pure protein moiety and the molecular weight was 2.5 KDa based on the plasmid curing experiment, suggesting that the bacteriocin was a plasmid encoded protein molecule. The study revealed the possibility of using bacteriocin as a food preservative and the L. plantarum strain as probiotic.[...] Read More.

Keywords: Antagonistic activity, bacteriocin, chromatography, Lactobacillus plantarum.

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Influence of growth medium on diagnostic characters of aspergillus and penicillium species

M. E. Zain, A. A. Razak, H. H. El-Sheikh, H. G. Soliman and A. M. Khalil

Abstract: Three fungal strains; namely, Aspergillus terreus, Penicillium janthinellum and Penicillium duclauxii were cultured on different growth media including yeast extract, malt extract, yeast-malt extract, Czapek's Dox, Sabourod's, Harrlod's, and potato dextrose. The growth and secondary metabolites of the three fungal strains were greatly affected by the growth medium. The colour of the culture and secondary metabolites were noticeably altered and changed according to the growth medium used.[...] Read More.

Keywords: Growth medium, Aspergillus, Penicillium, Secondary metabolites, Culture characteristics.

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Beneficial uses of probiotics in mass scale production of marine ornamental fish

Shanmugam Velmurugan* and Santhanam Rajagopal

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Production dynamics of Bacillus subtilis strain AG-1 and EAG-2, producing moderately alkaline proteases

Afia Ghafoor* and Shahida Hasnain

Abstract: Bacillus strains isolated from different habitats were screened and identified for high protease activity. Two of the bacillus strains genetically identified as Bacillus subtilis AG-1 and Bacillus subtilis EAG-2 exhibited tremendous potential for protease production. The strains were optimized with respect to production media and its components. Culture conditions were critically investigated with reference to other key determinants, involving inoculum concentration, incubation time, initial pH of the medium and temperature. The optimum production pH and temperature was found to be 7.0 and 35oC for B. subtilis AG-1, while 7.2 and 37oC were the optimum conditions for B. subtilis EAG-2. The inoculation ratio and incubation time were determined for both of the strains as 1% (v/v) and 24-32 h respectively. The study reveals that, high protease yield can be achieved over a moderate fermentation period with relatively economical production costs. Hence these strains might be useful for commercial exploitation.[...] Read More.

Keywords: Bacillus subtilis, protease, production medium, pharmamedia, optimum growth.

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Screening for bacteria agents responsible for otitis media and their antibiogram

Oyeleke, S. B.

Abstract: 30 samples (male and female) of ear swab were collected from patients at general hospital Minna for examination of bacteria that causes otitis media. 26 samples were positive for these organisms. The organisms isolated include Pseudomonas sp., Kleblesella sp., Proteus sp., Escherichia coli and Staphylococcus aureus. Pseudomonas sp., (54%) had the highest incidence of occurrence followed by Kleblesella sp. (19%) and Proteus sp (15%) while the least was S. aureus (4%). The organisms’ sensitivity test reveals that they were highly sensitive to gentamycin. The bacteria responsible for otitis media are pathogenic, therefore effort should be made to reduce the factors militating its occurrence of these pathogens in the community.[...] Read More.

Keywords: Otitis media, eustachian tube, salpingitis, gentamicin, prevalence, pathogenic.

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Evaluation of Securidaca longipenduculata leaf and root extracts for antimicrobial activities

Junaid S. A. *, Abubakar A. , Ofodile A. C. , Olabode A. O. , Echeonwu G. O. N. , Okwori A. E. J. and Adetunji J. A.

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Multidrug resistance in Pseudomonas aeruginosa: Insights into the molecular mechanisms

Abstract: Pseudomonas aeruginosa is commonly found in chronic lung infections such as cystic fibrosis and others. Intrinsic antibiotic resistance of P. aeruginosa accompanied by its ability to acquire resistance via mutations and adapt to the heterogeneous and dynamic environment of chronically infected lungs are major threats and reasons for the ultimate failure of the current antibiotic therapies in eradicating the infection from lungs. New insights at molecular levels in the process of accumulating such beneficial mutations at faster rates, termed as hypermutation have allowed us to understand the high acquired resistance of this opportunistic pathogen. Also, these understandings will allow us to develop new therapeutic strategies to combat chronic infections.[...] Read More.

Keywords: Pseudomonas aeruginosa, chronic lung infections, multi drug resistance, hypermutation.

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Molecular investigation of Streptococcus agalactiae isolates from environmental samples and fish specimens during a massive fish kill in Kuwait Bay

Qasem A. Jafar *, Al-Zinki Sameer , Al-Mouqati Salwa , Al-Amad Samee , Al-Marzouk Ahmed and Al -Sharifi Faisal

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Differentiation of parasitic and saprophytic leptospirae by oxidase test â€“ A hasty modus operandi

Differentiation of parasitic and saprophytic leptospirae by oxidase test – A hasty modus operandi

Abstract: The use of the cytochrome oxidase test reagent, as a differential agent to study the pathogenic and saprophytic leptospires was studied. Growth of the leptospiral strains of the saprophytic group was almost involved in the conversion of colorless tetramethyl p-phenelene diamine dihydrochloride solution to dark brown color whereas pathogenic leptospirae strains showed reddish brown color. Saprophytic strains required 20-35 min for oxidation of cytochrome, however, pathogenic strains required more time, about 45 min for cytocrome oxidation. By this preliminary investigation, it is now possible to differentiate and report the saprophytic and parasitic strains based on the differential observation of the cytochrome oxidase test.[...] Read More.

Keywords: Pathogenic, leptopsiral strains, saprophytic strain, 1% oxidase reagent, rapid detection.

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Evidence for probiotic potential of a capsular-producing Streptococcus thermophilus CHCC 3534 strain

Rowaida K. Khali

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