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Full Length Research Paper
Abstract: The effect of soyabeans fortification on acceptability and shelf life of ogi was investigated. The pH of the fermenting ogi decreased steadily with a corresponding increase in titratable acidity (TA). The pH and TA ranged between 3.03 and 4.30; and 1.07 and 1.54 (mg/100 g) respectively. During fermentation, a significant increase in yeast and lactic acid bacteria (LAB) counts was observed. The aerobic plate count (log cfu/ml) of fermenting ogi samples ranged between 5.96 and 5.99. Sample A containing sorghum, millet and soybeans had the highest protein content (9.2%) while sample D containing sorghum only had the lowest (8.16%). Sample D was rated best in all the parameters tested. This study revealed that fortification with soybean at 10% level and a combination of sorghum and millet improved the nutrition quality of ogi without adverse effect on acceptability.[...] Read More.
Keywords: Nutritional quality, soybean, fortification, composite ogi, acceptability.
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文章
Noor-Zarina Abdul-Wahab, Saleha Shahar, Halimah Abdullah-Sani, Azimahtol Hawariah Lope Pihie and Nazlina Ibrahim*
Abstract: The antioxidant, antibacterial, and antiviral effects of crude methanol extract from the leaves of Goniothalamus umbrosus were studied. Good free radical scavenging activity using 1,1- diphenyl-2-picrylhydrazyl (DPPH) assay was shown by the extract at EC50 of 0.263 mg/ml compared to 0.01 mg/ml for trolox as the positive control. The extract showed greater antibacterial activity against Gram-positive bacteria than Gram-negative with methicillin resistant Staphylococcus aureus (MRSA) isolates were more sensitive than methicillin sensitive S. aureus. Cytotoxicity testing of the leaf extract and goniothalamin a styrylpyrone derivative (SPD) as a lead compound found mostly in the genus Goniothalamus was performed on Vero cells. From the test, the CC50 value for the extract was 125 µg/ml and for SPD 31.25 µg/mL (1.563x10-7 µM). Antiviral test was carried out against Herpes Simplex virus type 1 infected to Vero cells. The results showed that G. umbrosus leaf methanol extract was effective in showing the antiviral activity in all three treatments but SPD is effective only in the first treatment; 1) (C+V)+E, extract and SPD were able to control virus post-infection 2) (C+E)+V: the extract has prophylactic activity implied by treatment of cells with the extract for one day before viral infection and C+(V+E) refers to the ability of extract to control viral infectivity when extract and virus was added simultaneously to cell. This study showed the potential of non-cytotoxic G. umbrosus leaf methanol extract as herbal alternative against MRSA and herpes simplex viruse (HSV) -1.[...] Read More.
Keywords: Goniothalamus umbrosus, leaf, methanol extract, styrylpyrone derivative, antioxidant, antibacterial, cytotoxicity, antivirus.
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文章
Full Length Research Paper
Abstract: Isolation and characterization of new LACTOBACILLI strains with high level acetaldehyde production, from traditional fermented milk, in different provinces of Iran, are reported. In this study, a total of 112 local dairy products such as yoghurt and doogh were collected from four provinces in the west of Iran, namely Kordestan, Kermanshah, Ilam and Lorestan. Ninety three isolates with extensive diverse phenotypic and biochemical characteristics were identified. These isolates were screened for productions of acetaldehyde during fermentation of skim milk. 67 isolate were positive for acetaldehyde production. Two strains (KR43 and IL121) with the highest acetaldehyde production were further characterized by amplification and sequencing of 16S rRNA genes and their phylogenetic trees were constructed. The results showed that the two strains were similar to LACTOBACILLUS FERMENTUM. 2-dimensional electrophoresis analysis was used to investigate the proteome of the cytosolic fraction extracted from stationary phase cells of the KR43 strain. Also some spots of KR43 strain were analyzed by MS/MS subunits of acetoin dehydrogenase involved in the production of acetaldehyde from acetoin, which has not been reported in LACTOBACILLI yet. To our knowledge, this is the first report in LACTOBACILLI.[...] Read More.
Keywords: Acetaldehyde production, acetoin dehydrogenase, Lactobacillus, Iran, proteomics, fermented foods, dairy products.
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文章
Izham N. S. A. S., H. Zuridah, *, S. Rashidah, O. Azizon and N. A. Zeenathul
Abstract: The role of Epstein-Barr virus (EBV) as a cofactor in breast cancer is controversial and its association with breast cancer varies. In this study, EBV was detected by using in situ hybridization technique (ISH) to detect Epstein-barr virus encoded RNA1 (EBER1) transcripts. Archival formalin-fixed paraffin embedded breast cancer tissue samples (n = 139) and normal breast tissue (n = 20) obtained from Hospital Tuanku Ja’afar were sectioned, stained and examined microscopically for nuclear staining and by DNA amplification of the same gene. By ISH, 83/139 (59.7%) and 12/20 (60%) were EBV positives in the breast cancer tissues and normal tissues, respectively. On the other hand, confirmation by polymerase chain reaction (PCR) found that additional six samples (89/139 or 64%) breast cancer tissues were positive for EBER1 gene. To further confirm the identity of these amplified products, two samples (UiTM-53 and UiTM-73) were sequenced, BLAST, analyzed phylogenetically and was found to be 100% similar to the EBV EBER1 gene sequences already deposited in the GenBank (accession numbers AB065135, FN545286, EF187853 and DQ883818). These preliminary findings suggest that there is a correlation between EBV and breast cancer but need further testing with more samples to confirm the role of EBV.[...] Read More.
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文章
M. A. Latif,*, M. M. Rahman, M. S. Kabir, M. A. Ali, M. T. Islam and M. Y. Rafii
Abstract: Genetic diversity was studied for blast resistant and susceptible genotypes using 13 morphological characters. Plant height, days to flowering and maturity, panicle length, number of spikelet per panicle, number of filled grain per panicle, number of unfilled grain per panicle, 1000-grain weight and yield per hill were indicated as important contributors to genetic divergence in 14 rice genotypes. The first 3 principal components accounted for 78.72% of the total variation among resistant and susceptible rice genotypes. The genotypes were grouped into 7 clusters according to Mohalanobis’s D2 statistics and canonical vector analysis. On the basis of cluster distances, high yielding along with highly susceptible genotype, Bangladesh Rice Research Institute (BRRI) dhan29 could be crossed with resistant genotypes, BR 6017-3-3-4-1 and Zong-yu 7. Similarly, BRRI dhan28 could be crossed with Qing Liali No.1 for the development of blast resistant rice varieties with higher yield.[...] Read More.
Keywords: Blast disease, genetic diversity, quantitative traits, rice, resistant genotypes.
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文章
Atanda S. A*, Pessu P. O., Agoda S., Isong I. U., Adekalu O. A., Echendu M. A. and Falade T. C.
Abstract: This review focused on the worldwide contamination of foods and feeds with mycotoxins as a significant problem. Mycotoxins are secondary metabolites of molds that have adverse effects on humans, animals, and crops that result in illnesses and economic losses. Aflatoxins, ochratoxins, trichothecenes, zearelenone, fumonisins, tremorgenic toxins, and ergot alkaloids are the mycotoxins of greatest agro-economic importance. Factors influencing the presence of mycotoxins in foods or feeds include environmental conditions related to storage that can be controlled. Other extrinsic factors such as climate or intrinsic factors such as fungal strain specificity, strain variation, and instability of toxigenic properties are more difficult to control. The economic impact of mycotoxins include loss of human and animal life, increased health care and veterinary care costs, reduced livestock production, disposal of contaminated foods and feeds, and investment in research and applications to reduce severity of the mycotoxin problem. Although efforts have continued internationally to set guidelines to control mycotoxins, practical measures have not been adequately implemented.[...] Read More.
Keywords: Fungi, mycotoxins, aflatoxins, contamination, relative humidity, safe moisture content.
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文章
Aphidech Sangdee*, Sarawut Sachan and Surasak Khankhum
Abstract: Anthracnose disease is one of the major economic constraints to chilli production in tropical and subtropical regions. Ten isolates of Colletotrichum capsici causing chilli anthracnose were collected from 10 provinces in the northeast of Thailand. The isolates were evaluated for their morphological and cultural characteristics, pathogenic variability on chilli fruits and genetic characterization using random amplified polymorphic DNA (RAPD-PCR). Based on the morphological traits and cultural characteristics of the C. capsici populations, 10 isolates were categorized into six groups. These were designated, respectively, as CC-I, CC-II, CC-III, CC-IV, CC-V and CC-VI. In Potato Dextrose Agar culture, most of the isolates produced cottony colonies. However, differences were obtained in colony color, shape and size of conidia. Based on the effect of carbendazim, 10 isolates were classified into two groups designated as highly resistant group (90% inhibition). Three virulence degrees of 10 isolates on chilli fruits were evaluated. Molecular polymorphism generated by RAPD confirmed the variation of the different isolates and they were grouped into two clusters. However, morphological, pathological and RAPD grouping of isolates suggested no correlation among the test isolates.[...] Read More.
Keywords: Colletotrichum capsici, morphological and cultural characteristics, pathogenic variability, random amplified polymorphic DNA (RAPD).
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文章
Hami Kaboosi
Abstract: According to the definition of probiotics by the Food and Agriculture Organization of the United Nations/World Health Organization, “probiotics are live microorganisms, which when administered in adequate amounts confer a health benefit on the host”. Microorganisms that are probiotics in humans include Enterococci, Bifidobacteria and lactic acid bacteria, such as Lactobacilli, Lactococci and Streptococci. This research was conducted to determine the presence of antibacterial effects among the probiotics isolated from different bioyoghurts against some common bacterial pathogens. Lactobacillus sp., Streptococcus sp. and Bifidobacterium sp. from yoghurts containing probiotics were isolated and examined for their antibacterial effects against Staphylococcus aureus, Escherichia coli, Salmonella typhi, and Pseudomonas aeruginosa. The modified agar overlay method was used for determination of the presence of antibacterial effects among the isolated probiotics. Results showed the presence of antibacterial effects among the probiotics that were isolated from bioyoghurts. The spectrum of their antibacterial effects varied against the selected pathogen. Antibacterial effects are one of the most important selection criteria for probiotics, and the verified antibacterial activity of the probiotics supports the development of these functional foods as a key to the improvement of health in the consuming public.[...] Read More.
Keywords: Probiotics, bioyoghurts, Lactobacillus spp., Bifidobacterium spp., antibacterial effects.
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文章
Myriam BEN SAID*, Sana KHEFACHA, Lobna MAALEJ, Imen DALY and Abdennaceur HASSEN
Abstract: Pseudomonas aeruginosa is a commonly found nosocomial opportunistic pathogen that is characterized by its ability to form biofilm to protect itself from various external pressures. Biofilms are problematic in particular food industry sectors and in water distribution systems. In this study, we propose to explore the impact of UVC dose on biofilm production by P. aeruginosa strains. The biofilms densities were assessed by crystal violet assay. A relationship between UVC exposed dose and biofilm density was examined by establishment of new model linking between UVC dose and physiology bacteria response concerning biofilm production (UVC dose/ biofilm production).[...] Read More.
Keywords: UVC dose, biofilm, P. aeruginosa, quorum sensing, RecA, model.
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文章
M. Monajjemi*, R. Hasani, R. Rasoolzadeh and F. Mollaamin
Abstract: 14-O-demethyledelboxine is one of the most important alkaloids in Consolida species. It is toxic and has many effects in body. In this article, we used dipole moment and nuclear magnetic resonance (NMR) to describe this molecule properties. Nanoparameters and chemical shift calculations, geometry optimization and energies have been performed with ab initio method at HF/Sto-3G, B3Lyp/6-31G and B3Lyp/6-31G* levels with magnetic properties of the gauge-including atomic orbital method. According to this study this molecule has polarity, therefore it can highly react with microtubules. So it is recommended that more study in this molecule and the groups can be bound to evaluate these characters so it could be useful. Dipole moment calculations approve ab initio methods according to these results.[...] Read More.
Keywords: 14-o-demethyldelboxine, nuclear magnetic resonance (NMR), B3lyp, isotropy, anisotropy.
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