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Izham N. S. A. S., H. Zuridah, *, S. Rashidah, O. Azizon and N. A. Zeenathul
Abstract: The role of Epstein-Barr virus (EBV) as a cofactor in breast cancer is controversial and its association with breast cancer varies. In this study, EBV was detected by using in situ hybridization technique (ISH) to detect Epstein-barr virus encoded RNA1 (EBER1) transcripts. Archival formalin-fixed paraffin embedded breast cancer tissue samples (n = 139) and normal breast tissue (n = 20) obtained from Hospital Tuanku Ja’afar were sectioned, stained and examined microscopically for nuclear staining and by DNA amplification of the same gene. By ISH, 83/139 (59.7%) and 12/20 (60%) were EBV positives in the breast cancer tissues and normal tissues, respectively. On the other hand, confirmation by polymerase chain reaction (PCR) found that additional six samples (89/139 or 64%) breast cancer tissues were positive for EBER1 gene. To further confirm the identity of these amplified products, two samples (UiTM-53 and UiTM-73) were sequenced, BLAST, analyzed phylogenetically and was found to be 100% similar to the EBV EBER1 gene sequences already deposited in the GenBank (accession numbers AB065135, FN545286, EF187853 and DQ883818). These preliminary findings suggest that there is a correlation between EBV and breast cancer but need further testing with more samples to confirm the role of EBV.[...] Read More.
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M. A. Latif,*, M. M. Rahman, M. S. Kabir, M. A. Ali, M. T. Islam and M. Y. Rafii
Abstract: Genetic diversity was studied for blast resistant and susceptible genotypes using 13 morphological characters. Plant height, days to flowering and maturity, panicle length, number of spikelet per panicle, number of filled grain per panicle, number of unfilled grain per panicle, 1000-grain weight and yield per hill were indicated as important contributors to genetic divergence in 14 rice genotypes. The first 3 principal components accounted for 78.72% of the total variation among resistant and susceptible rice genotypes. The genotypes were grouped into 7 clusters according to Mohalanobis’s D2 statistics and canonical vector analysis. On the basis of cluster distances, high yielding along with highly susceptible genotype, Bangladesh Rice Research Institute (BRRI) dhan29 could be crossed with resistant genotypes, BR 6017-3-3-4-1 and Zong-yu 7. Similarly, BRRI dhan28 could be crossed with Qing Liali No.1 for the development of blast resistant rice varieties with higher yield.[...] Read More.
Keywords: Blast disease, genetic diversity, quantitative traits, rice, resistant genotypes.
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Atanda S. A*, Pessu P. O., Agoda S., Isong I. U., Adekalu O. A., Echendu M. A. and Falade T. C.
Abstract: This review focused on the worldwide contamination of foods and feeds with mycotoxins as a significant problem. Mycotoxins are secondary metabolites of molds that have adverse effects on humans, animals, and crops that result in illnesses and economic losses. Aflatoxins, ochratoxins, trichothecenes, zearelenone, fumonisins, tremorgenic toxins, and ergot alkaloids are the mycotoxins of greatest agro-economic importance. Factors influencing the presence of mycotoxins in foods or feeds include environmental conditions related to storage that can be controlled. Other extrinsic factors such as climate or intrinsic factors such as fungal strain specificity, strain variation, and instability of toxigenic properties are more difficult to control. The economic impact of mycotoxins include loss of human and animal life, increased health care and veterinary care costs, reduced livestock production, disposal of contaminated foods and feeds, and investment in research and applications to reduce severity of the mycotoxin problem. Although efforts have continued internationally to set guidelines to control mycotoxins, practical measures have not been adequately implemented.[...] Read More.
Keywords: Fungi, mycotoxins, aflatoxins, contamination, relative humidity, safe moisture content.
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Aphidech Sangdee*, Sarawut Sachan and Surasak Khankhum
Abstract: Anthracnose disease is one of the major economic constraints to chilli production in tropical and subtropical regions. Ten isolates of Colletotrichum capsici causing chilli anthracnose were collected from 10 provinces in the northeast of Thailand. The isolates were evaluated for their morphological and cultural characteristics, pathogenic variability on chilli fruits and genetic characterization using random amplified polymorphic DNA (RAPD-PCR). Based on the morphological traits and cultural characteristics of the C. capsici populations, 10 isolates were categorized into six groups. These were designated, respectively, as CC-I, CC-II, CC-III, CC-IV, CC-V and CC-VI. In Potato Dextrose Agar culture, most of the isolates produced cottony colonies. However, differences were obtained in colony color, shape and size of conidia. Based on the effect of carbendazim, 10 isolates were classified into two groups designated as highly resistant group (90% inhibition). Three virulence degrees of 10 isolates on chilli fruits were evaluated. Molecular polymorphism generated by RAPD confirmed the variation of the different isolates and they were grouped into two clusters. However, morphological, pathological and RAPD grouping of isolates suggested no correlation among the test isolates.[...] Read More.
Keywords: Colletotrichum capsici, morphological and cultural characteristics, pathogenic variability, random amplified polymorphic DNA (RAPD).
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Hami Kaboosi
Abstract: According to the definition of probiotics by the Food and Agriculture Organization of the United Nations/World Health Organization, “probiotics are live microorganisms, which when administered in adequate amounts confer a health benefit on the host”. Microorganisms that are probiotics in humans include Enterococci, Bifidobacteria and lactic acid bacteria, such as Lactobacilli, Lactococci and Streptococci. This research was conducted to determine the presence of antibacterial effects among the probiotics isolated from different bioyoghurts against some common bacterial pathogens. Lactobacillus sp., Streptococcus sp. and Bifidobacterium sp. from yoghurts containing probiotics were isolated and examined for their antibacterial effects against Staphylococcus aureus, Escherichia coli, Salmonella typhi, and Pseudomonas aeruginosa. The modified agar overlay method was used for determination of the presence of antibacterial effects among the isolated probiotics. Results showed the presence of antibacterial effects among the probiotics that were isolated from bioyoghurts. The spectrum of their antibacterial effects varied against the selected pathogen. Antibacterial effects are one of the most important selection criteria for probiotics, and the verified antibacterial activity of the probiotics supports the development of these functional foods as a key to the improvement of health in the consuming public.[...] Read More.
Keywords: Probiotics, bioyoghurts, Lactobacillus spp., Bifidobacterium spp., antibacterial effects.
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Myriam BEN SAID*, Sana KHEFACHA, Lobna MAALEJ, Imen DALY and Abdennaceur HASSEN
Abstract: Pseudomonas aeruginosa is a commonly found nosocomial opportunistic pathogen that is characterized by its ability to form biofilm to protect itself from various external pressures. Biofilms are problematic in particular food industry sectors and in water distribution systems. In this study, we propose to explore the impact of UVC dose on biofilm production by P. aeruginosa strains. The biofilms densities were assessed by crystal violet assay. A relationship between UVC exposed dose and biofilm density was examined by establishment of new model linking between UVC dose and physiology bacteria response concerning biofilm production (UVC dose/ biofilm production).[...] Read More.
Keywords: UVC dose, biofilm, P. aeruginosa, quorum sensing, RecA, model.
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文章
M. Monajjemi*, R. Hasani, R. Rasoolzadeh and F. Mollaamin
Abstract: 14-O-demethyledelboxine is one of the most important alkaloids in Consolida species. It is toxic and has many effects in body. In this article, we used dipole moment and nuclear magnetic resonance (NMR) to describe this molecule properties. Nanoparameters and chemical shift calculations, geometry optimization and energies have been performed with ab initio method at HF/Sto-3G, B3Lyp/6-31G and B3Lyp/6-31G* levels with magnetic properties of the gauge-including atomic orbital method. According to this study this molecule has polarity, therefore it can highly react with microtubules. So it is recommended that more study in this molecule and the groups can be bound to evaluate these characters so it could be useful. Dipole moment calculations approve ab initio methods according to these results.[...] Read More.
Keywords: 14-o-demethyldelboxine, nuclear magnetic resonance (NMR), B3lyp, isotropy, anisotropy.
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Moussa I. M.*, Kh. F. Mohamed, Marwah Mohamed, Nasr E. A., Atef M. Shibl, Mounir M. Salem-Bekhit and Hatem M. E.
Abstract: Polymerase chain reaction (PCR) are rapid and simple means for the differentiation of members of Mycobacterium tuberculosis complex, especially Mycobacterium bovis and M. tuberculosis, where it is important to distinguish between zoonotic sources (cattle and unpasteurized dairy products) and human sources of tubercle disease. This study is aimed to evaluate the recent technique such as PCR and (BACTEC MGIT 960 TM system) for diagnosis of M. tuberculosis complex among cattle in Egypt. 1180 cattle were examined during the period of 2008 to 2010 by single intradermal tuberculin test. 29 animals (2.46%) were positive reactors, the results of isolation and identification using conventional culture method Lowenstein-Jensen medium were 22 mycobacterial isolates (75.9%), 20 (68.97%) M. bovis and 2 (6.9%) unidentified slow grower}. The recovery rate of BACTEC MGIT 960TM system was 82.8%, while in case of Lowenstein-Jensen medium was 75.9%. The mean time for detection of Mycobacterium was 17.8 ± 0.9 days and 46.5 ± 0.4 days for BACTEC MGIT 960TM system and Lowenstein-Jensen medium, respectively. While the contamination rate with BACTEC MGIT 960TM system was 6.9 and 10.3% in Lowenstein-Jensen medium. PCR technique in the present study could differentiates between M. bovis and M. tuberculosis within few h rather than the long period required for the biochemical identification tests. Therefore, the use of PCR in the diagnosis of Mycobacterium in clinical samples is as rapid, more reliable, sensitive and specific techniques and can be used for large scale screening of Mycobacterium in areas where the disease is still a public health hazard as in Egypt.[...] Read More.
Keywords: Bovine tuberculosis, tuberculin test, Lowenstein-Jensen medium, BACTEC system, PCR.
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文章
Abdul Rehman, Kishwar Sultana, Nisar Minhas, Muhammad Gulfraz, Ghazala Kaukab Raja* and Zahid Anwar
Abstract: To study most prevalent wheat seed-borne mycoflora and its effect on the nutritional value, freshly harvested three and six months old stored wheat grains were collected from various parts of Pakistan. For isolation of seed-borne fungi, agar plate method was found more effective as compared to standard blotter method. Alternaria alternata was found to be the most prevalent fungus. Chemical analysis of seeds clearly showed a decrease in the carbohydrate, fats and ash contents of stored wheat grains as compared to the freshly harvested seeds with no effect on total proteins. The growth of A. alternata on seeds during storage might have resulted in low nutritional contents.[...] Read More.
Keywords: Mycoflora, Alternaria alternata, seed-borne fungi, post-harvest storage, pathogens.
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文章
Qing-Ping Hu and Jian-Guo Xu*
Abstract: Siderophores, a high-affinity chelating agents for ferric iron, is one of the mechanisms by which biocontrol agents’ act in inhibiting the growth of phytopathogens. Bacillus subtilis QM3, a siderophore producer, is a potential biocontrol agent. In this study, a simple double-layered chrome azurol S agar (SD-CASA) plate assay was developed. We further studied optimal conditions for detection siderophore production by B. subtilis QM3 using various history cultures, pH value, amino acid, and different carbon-nitrogen ratio as four variables. After incubating different history cultures we found out that the LB medium presented strong significant CAS reaction even for 12 h, and with the help of changing variables on SD-CASA plate assisting paper-disc diffusion, strain QM3 from LB medium with Fe3+ (10 μmol/L) showed difference interestingly. In addition, CAS reaction and growth reaction also increased with pH 7.0, tryptophan 10 μmol/L and carbon-nitrogen ratio 5:1. The findings on solid medium were similar as that in liquid medium. The SD-CASA assay should be a useful tool for screening potential biocontrol microorganisms by siderophore production.[...] Read More.
Keywords: A simple double-layered CAS agar assay, paper-disc diffusion, siderophores, Bacillus subtilis QM3.
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