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文章
Short Communication
Abstract: Endophytic Fusarium species were isolated from roots of wild banana (Musa acuminata) collected randomly in several locations in Penang Island, Malaysia. A total of 54 isolates of Fusarium were recovered from 100 root fragments. Based on morphological features of macroconidia, microconidia and conidiogenous cells, three Fusarium species were identified in which the most common species was F. oxysporum (41.5%) followed by F. solani (32.1%) and F. semitectum (24.5%). The present study showed that there are diverse endophytic Fusarium species in the roots of wild banana and has the potential to be used as biological control agents.[...] Read More.
Keywords: Endophyte, Fusarium, root, wild banana.
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文章
Wang Chun-guang*, Zhang Tie, and Zhang Jian-lou
Abstract: Drug resistance is widely distributed in Escherichia coli coli, and related technology is urgently required to control its transmission. The Chinese pulsatilla extracts containing crude drug of 12.5 mg/ml was used to eliminate resistance plasmid against streptomycin in chicken E. coli. After screening and identification, plasmids were extracted from resistance-eliminated strain and electrophoresed in order to compare plasmid profiles before and after elimination. After the drug resistance was eliminated, the diameter of inhibition zone became larger and the minimum inhibitory concentration declined. These results indicated that Chinese pulsatilla extracts makes E. coli strain that was sensitive to streptomycin become resistant to this antibiotic. The alimentation rate was 15.8% (79/500). Compared with the control bacteria, the resistance-eliminated strain lost an approximately 20 kbp plasmid band, which showed that the Chinese pulsatilla extracts may convert drug resistance by eliminating plasmid against streptomycin.[...] Read More.
Keywords: Chinese pulsatilla, Escherichia coli, streptomycin, resistance plasmid.
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文章
H. Zuridah,*, N. Norazwin, M. Siti Aisyah, M. N. A. Fakhruzzaman and N. A. Zeenathul
Abstract: Lipase enzyme is an important group of biocatalyst for biotechnological application. One of the major industrial microorganism is the Bacillus species which are in abundance in the hot springs. The aim of this research was to isolate and characterize lipase producing bacteria from five hot springs in Malaysia. Eight bacterial strains from five hot springs were characterized by both biochemical and molecular techniques. Four strains were lipase producing thermophilic bacteria (coded as A1, A3, A4 and A14). The presence of lipase enzyme was confirmed by an orange fluorescence halos around the bacteria in rhodamine B olive oil agar plate. Based on molecular analysis, A1, A3 and A4 were assigned to the Bacillus and Geobacillus sp., and isolate A14 is found to be a nucleoside permease gene from Anoxybacillus flavithermus WK1.[...] Read More.
Keywords: Lipase enzyme, rhodamine B olive oil, polymerase chain reaction (PCR), hot springs, thermophilic bacteria.
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文章
Golnaz Shams*, Mohammad Hassan Safaralizadeh and Sohrab Imani
Abstract: Laboratory experiments were carried out in order to evaluate the insecticidal effect of diatomaceous earth against adults of Callosobruchus maculatus (F.) and Sitophilus granarius (L.), two important stored grain pests in darkness (30°C and 65% RH). Wheat and cowpea were treated with the diatomaceous earth formulation Silicosec® (Biofa, Germany), at five concentrations determined with preliminary tests and compared with untreated wheat and cowpea as control. Dead adults were counted 24, 36 and 48 h later. Results showed that increasing the concentration of Silicosec® and days after treatment (DAT) significantly increased the mortality rates of adults to above 90% in both experiments. Regarding Lc50 and Lc95 values, it was observed that C. maculatus adults are more susceptible to Silicosec® than S. granarius. The results showed that these two pests could be controlled successfully with diatomaceous earth.[...] Read More.
Keywords: Callosobruchus maculatus, diatomaceous earth, Silicosec®, Sitophilus granarius, stored grains, cowpea, wheat.
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Jumin Hao, Jiang Zheng*, Xuemin Tang, Qingpi Yan, Yubao Li and Zhongbao Li
Abstract: We have explored a simplified system without purification for systematic evolution of ligands by exponential enrichment (SELEX) against Vibrio alginolyticus to select single-stranded DNA ligands (aptamers) from a random 82-nt library. The DNA content was quantified by agarose gel electrophoresis and follow-up image analyses with the BandScan 5.0 software. Anti-digoxigenin/HRP system was used to determine the binding activity of aptamers for V. alginolyticus. The results showed that the affinity of aptamers increased gradually with the increase of screening rounds, indicating that the lack of purification did not affect screening results but rather made SELEX screening much more convenient and significantly increased the efficiency.[...] Read More.
Keywords: Aptamers, purification, systematic evolution of ligands by exponential enrichment (SELEX), Vibrio alginolyticus.
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文章
Sepideh Mostofi, Reza Mirnejad* and Faramaz Masjedian
Abstract: The aim of this study is to investigate multi-drug resistance in A. baumannii strains that was isolated from clinical samples of three highly large hospitals in Tehran-Iran. This descriptive-cross sectional study was performed in three large hospitals in Tehran on 70 samples of Acinetobacter which were isolated from patients during April to November 2010. After identifying the species level by using culture and biochemical methods, in order to determine sensitivity of 50 isolates of A. baumannii to 13 antibiotics, standard methods according to CLSI guidelines were performed. In this study, resistance to three or more of three classes of antibiotics multidrug resistance was defined. In this study, 50 A. baumannii strains, 12 A. lwoffii strains and other Acinetobacter species were isolated from patients. The majority of isolates were from blood specimens. Isolates of A. baumannii showed the highest resistance to cefepime, ceftazidime, aztreonam, norfloxacin, ofloxacin, ciprofloxacin and amikacin. Tobramycin and meropenem considered as effective drugs in this study. Multi-drug resistance in these strains was respectively 55.4%. Multi-drug resistant Acinetobacters are growing and considered as important threat for hospitalized patients, so change in consumption patterns of antibiotics and control of hospital infections seems to be necessary.[...] Read More.
Keywords: Acinetobacter baumannii, nosocomial infection, multi-drug resistance, antibiogram.
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文章
Rakib A. Al-Ani, Mustafa A. Adhab* and Kareem A. Hassan
Abstract: Eggplant (Solanum melongena) is an important vegetable and widely cultivated in polyethylene covered houses in Iraq. A new virus, Eggplant blister mottled virus (EBMV), was isolated in previous study, from naturally infected eggplant and was characterized as potyvirus. The virus is considered now as the most important virus infecting eggplants and causing heavy damage to yields. This study was conducted to evaluate the effect of three products, Vit-org nutrient, 2-nitromethyl phenol, and Thuja extract on the multiplication of EBMV in eggplants. Results of the study showed that the application of the three products on EBMV-inoculated eggplants at 2.5 and 1 mlL, and 6 gL caused a reduction in ELISA reactions absorbance to 0.073, 0.091, and 0.092, respectively, which reflect a reduction in virus concentration. The application of the products on eggplants at the same concentrations followed by virus inoculation, after 24 h of protecting the plants against virus infection for 18, 10 and 12 days, respectively accompanied by retardation of symptoms development in 26, 16 and 20 days for the three products, respectively. The treated eggplants neither developed visible symptoms nor contained detectable concentrations of virus during these periods. Vit-org nutrient was found to be more efficient in protecting the plants against EBMV in both post and pre-applications.[...] Read More.
Keywords: Eggplant blister mottled virus (EBMV), eggplant, Thuja extract, Vit-org, 2-nitromethyl phenol.
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文章
Abdulahi Mohamoud, Esaya Tessema and Hailu Degefu*
Abstract: A cross-sectional seroprevalence study of cattle foot and mouth disease (FMD) was conducted in Somalia Regional State (Awbere and Babille Districts) Western, Ethiopia. 384 blood samples were collected in the period of October 2009 to March 2010 from 384 animals and tested for antibodies against non-structural protein of FMD virus by using the 3ABC-ELISA. The overall individual animal antibody seroprevalence was 14.05% (95% CI = 11.2 to 18.13%). Statistically no significant variation (P > 0.05) was observed in the prevalence of FMD in Awbere (14.2%) and Babille (15.1%) Districts. Similarly there was no significant variation (P>0.05) in seroprevalence among male (19.4%) and female (13.6%) animals. Seroposetivity recorded for calves (Zero), young (13.2%) and adult (18.9%) age groups of animals showed a significance variation (χ2 = 8.45, DF = 2, P = 0.01). The results of this study showed that FMD is an important cattle disease in the study areas. This fact justifies the need of attention and subsequent study for identification of the FMD virus circulating in the area, which helps in the implementation of an effective control measures.[...] Read More.
Keywords: Cattle, Ethiopia, foot and mouth disease (FMD), Jijiga, Seroprevalence, 3ABC-ELISA.
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Full Length Research Paper
Abstract: Drymoglossum piloselloides (DP) is a small epiphytic fern commonly seen on trunks of older trees. The antimicrobial activity of water, ethanol and chloroform extracts of DP was tested against Trichophyton rubrum ATCC 40051 and Trichophyton mentagrophytes ATCC 40004 which are the two most commonly cause of Athlete’s foot, Candida albicans ATCC 14053, Candida tropicalis ATCC 14056, Microsporum canis (clinical isolate) and Aspergillus fumigatus ATCC 14109. The chloroform and ethanol extracts only had mild activity against the Trichophyton spp. and the water extract was devoid of any activity. The anti-fungal activity was statistically less potent than griseofulvin and fluconazole or itraconazole. The anti-bacterial activity of DP extracts was also tested against four bacterial strains using the disc diffusion and microdilution methods. Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Streptococcus pneumoniae ATCC 49619, Bacillus subtilis and Salmonella enteritidis (clinical isolates). The antibacterial activity against gram positive bacteria was detected only in water extract minimum inhibitory concentration (MIC) 12.5 mg/ml against S. aureus. The minimum bactericidal concentrations of 25 mg/ml against S. aureus. This antimicrobial activity was compared to standard antibiotics (ampicillin and chloramphenicol). The water extract had statistically less potent anti-bacterial activity when compared to ampicillin and chloramphenicol. The anti-fungal and anti-bacterial activity of DP was minimal this may be due to the testing of crude extracts. Future studies of purified or semi purified samples may produce better results and may potentially be used in the treatment of Athlete’s foot and the related secondary bacterial infections.[...] Read More.
Keywords: Drymoglossum piloselloides, antifungal, antibacterial, in vitro.
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Full Length Research Paper
Abstract: The yeast Malassezia pachydermatis is part of the normal cutaneous microflora of most homoeothermic vertebrates. However, under certain conditions as high humidity, seborrheic skin, corticotherapy and immunodeficiency, it can become pathogenic and cause dermatopathies. The pathogenic role of the genus Malassezia seems to be related to physical, chemical and immunological disturbances and to the production of enzymes, including lipases, phospholipases and hydrolases. The Api-Zym® system is a semi quantitative method designed for the study of 19 enzymatic activities of tissues, cell suspensions, biological fluids, microorganisms, soil and others. Herein, the use of the Api-Zym system in 30 M. pachydermatis isolates detected the presence of the phosphohydrolases: acid phosphatase and naphtol-AS-BI-phosphohydrolase among all isolates, independently of the clinical sign associated to the animal. The knowledge of the enzymatic profile of M. pachydermatis aims to contribute to the comprehension of the role of each enzyme in the pathogeny of this yeast.[...] Read More.
Keywords: Malassezia pachydermatis, enzymes, Api-Zym®, enzymatic profile, dogs.
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