By: Anna Ilyina*, Anita J. Casas-Reyes, Karla M. Gregorio-Jauregui, Erika Nava-Reyna, Jose L. Martinez-Hernandez, Nagamani Balagurusamy, Federico Cerda-Ramirez and Yolanda Garza-Garcia
Abstract: The present study focuses on the improvement of limitations of the bioluminescent ATP assay. An attempt was made to improve on the following: non-selective detection, limited cell population density quantification, and to distinguish between bioluminescent noises data and cell ATP, as a suitable technique for coliforms and ESCHERICHIA COLI detection, as well as other cell strains. Results were achieved by combining growth kinetics of the cell population exponential phase, in 2% brilliant green lactose bile broth wi…
Keywords: Bioluminescence; coliforms; Escherichia coli; exponential phase of growth; adenosine triphosphate
AJMR 2020, 19(1), 1-6;