Advanced Journal of Microbiology Research

Abstract: The role of Epstein-Barr virus (EBV) as a cofactor in breast cancer is controversial and its association with breast cancer varies. In this study, EBV was detected by using in situ hybridization technique (ISH) to detect Epstein-barr virus encoded RNA1 (EBER1) transcripts. Archival formalin-fixed paraffin embedded breast cancer tissue samples (n = 139) and normal breast tissue (n = 20) obtained from Hospital Tuanku Ja’afar were sectioned, stained and examined microscopically for nuclear staining and by DNA amplific…

Abstract: Isolation and characterization of new LACTOBACILLI strains with high level acetaldehyde production, from traditional fermented milk, in different provinces of Iran, are reported. In this study, a total of 112 local dairy products such as yoghurt and doogh were collected from four provinces in the west of Iran, namely Kordestan, Kermanshah, Ilam and Lorestan. Ninety three isolates with extensive diverse phenotypic and biochemical characteristics were identified. These isolates were screened for productions of acetal…

Abstract: The antioxidant, antibacterial, and antiviral effects of crude methanol extract from the leaves of Goniothalamus umbrosus were studied. Good free radical scavenging activity using 1,1- diphenyl-2-picrylhydrazyl (DPPH) assay was shown by the extract at EC50 of 0.263 mg/ml compared to 0.01 mg/ml for trolox as the positive control. The extract showed greater antibacterial activity against Gram-positive bacteria than Gram-negative with methicillin resistant Staphylococcus aureus (MRSA) isolates were more sensitive than…

Abstract: The effect of soyabeans fortification on acceptability and shelf life of ogi was investigated. The pH of the fermenting ogi decreased steadily with a corresponding increase in titratable acidity (TA). The pH and TA ranged between 3.03 and 4.30; and 1.07 and 1.54 (mg/100 g) respectively. During fermentation, a significant increase in yeast and lactic acid bacteria (LAB) counts was observed. The aerobic plate count (log cfu/ml) of fermenting ogi samples ranged between 5.96 and 5.99. Sample A containing sorghum, mille…

Abstract: The study was aimed at investigating the antimelanogenic and antioxidative effects of Bifidobacterium infantis culture filtrate. The efficacy of the bacterial culture filtrate was evaluated spectrophotometrically. The results revealed that B. infantis culture filtrate effectively scavenge 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) ABTS radicals, and show potent reducing power in a dose-dependent pattern. Additionally, the bacterial culture filtrate suppresses mu…

Abstract: Antimicrobial resistance has become a serious public health concern all over the world. The objective of this study was to determine susceptibility patterns of microorganisms to antibiotics in 11 hospital laboratories in Kurdistan province. During one month period (February, 2010), all the clinical specimens which were received from the laboratories were processed for isolation and identification of bacteria to the species level by standard methods. Testing procedures were validated following the Kirby-Bauer disc d…

Abstract: The objective of the present work was to perform the efficacy of one attenuated live vaccine Nobilis CAV P4 (Intervet Co., Netherland) in broiler breeder and their progenies compared to naturally infected flock. The vaccine was administrated to Ross 308 broiler breeder at 6 week old through S.C. rout. A total of 352 serum samples were collected from vaccinated and unvaccinated breeder flocks (that infected naturally) from 6 to 33 weeks old and 2 times in their progenies at one-day old. Sera were analyzed using indi…

Abstract: The occurrence of drug-resistant Vibrio cholerae is being reported with increasing frequency worldwide. Spread of resistant strains has been attributed, in part, sulfamethoxazole trimethoprim-constin (SXT-C). Sixty V. cholerae isolates obtained from cholera patients from different provinces in Iran during 2004 to 2006 and thirty-seven V. cholerae strains from surface water sources at 5 different locations in Tehran, Iran during 2006 were subjected to antibiotic susceptibility testing and polymerase chain reaction a…

Abstract: The anti-mitochondrial antifungal hypothesis implies a link between mitochondrial activity, fungal fruiting structures and susceptibility towards mitochondrial inhibitors. Here it is shown that the oomycete, Phytophthora nicotianae fits the hypothesis. Fruiting structures (zoosporangia) of this oomycete showed increased beta ( )-oxidation when probing levels of 3-hydroxy fatty acids (3-OH oxylipins) with specific polyclonal antibodies. In addition, increased mitochondrial activity was also observed in the zoosporan…

Abstract: Carbohydrates are the major nutrients of fruits and vegetables in human nutrition. Sucrose is one of the important parameters for the assessment of the commercial quality of the fruit, since consumers prefer the sweetest fruits. Results showed that fructose, glucose, sucrose contents were high in Cordia myxa (9.38, 12.75 and 29.09%) respectively and the starch content was high in Alocacia indica (60.41%). Alocacia has high calorie and nutritional value because it contains high carbohydrates contents (72.66%) and Co…

Abstract: Measurement of glycosylated hemoglobin has been proposed to be a very sensitive index for glycemic control as various proteins, including hemoglobin, albumin, collagen, low-density lipoprotein cholesterol (LDL), or crystalline proteins has been reported to undergo non-enzymatic glycation in diabetes. In the present study an attempt was made to elucidate the effect of aqueous root extract of Treculia africana on levels of haemoglobin glycosylation and plasma lipid peroxidation in streptozotocin (STZ),-induced diabet…

Abstract: A phylogenetic analysis of the Prorocentrum species is presented, that includes the sequences of the large and small ribosomal RNA subunits from 19 cultures from 13 of the 20 species reported in the Pacific coast of Mexico; the results showed that P. micans, P. gracile and P. mexicanum were the closest of species, that planktonic may be more recent than epibenthonic species and it is suggested that the probable ancestor of the Prorocentrum genus could be a round cell without apical spine, toxic and epibenthonic.[…

Abstract: It is well documented that aluminum (Al) toxicity is the most important constraint of crop production on acid soils. Chelation of Al in the rhizosphere with root secreted organic acid anions is a common mechanism of Al tolerance in most plants including soybean. Phenolic substances are recently implicated as additional physiological mechanism of plant Al tolerance. This study was undertaken to investigate the role of phenolics in soybean Al tolerance and to examine protein expression changes in soybean roots under…

Abstract: Six species of Sesbania were fingerprinted using RAPD and ISSR markers. Both markers yielded a total of 249 bands out of which 243 were polymorphic in nature indicating high degrees of genetic diversity in the genus. Cluster analysis using the combined data revealed segregation of the lone species Sesbania grandiflora from the rest of the species. High boot strap values in the dendrograms show the accuracy and authenticity of the result. All other five species got separated to a distinct cluster. This supports the…

Abstract: Random amplified polymorphic DNA (RAPD), Inter simple sequence repeat (ISSR) and Amplified fragment length polymorphism (AFLP) markers were used to verify the segregation of the genus Cassia L. (sens. lat.) into three distinct genera namely, Chamaecrista Moench., Senna P. Mill. and Cassia L. (sens.str.). Eighteen representatives of the three taxa were characterized using the molecular markers. 25 RAPD, six ISSR primers and six AFLP primer combinations resulted in the amplification of 612, 115 and 622 bands (loci)…