Advanced Journal of Microbiology Research

Abstract: The effect of the mutagen (acridine orange) on the multi-resistant antibiotics beta lactamase producing Staphylococcus aureus strain of bovine and human origin was investigated and tested individually using iodiometric (cell supervision) method for the level of beta-lactamase produced by each strain and antibiotic – sensitivity screening using single disc agar diffusion method. Analyses revealed that 80% of the isolates from bovine origin produced beta-lactamase while only 20% showed absence of the enzyme, while 68…

Abstract: Isoniazid resistant Mycobacterium tuberculosis strains are a serious threat for successful tuberculosis control programs. Therefore, present study was aimed to figure out the pattern and level of resistance of M. tuberculosis against isoniazid. A total of 172 specimens of sputum, pus and bronchial washings; 70.9% males and 29.1% females with 84.30% pulmonary and 15.69% extrapulmonary tuberculosis were collected from six different sources. The inoculums were prepared using 0.5 Mac Farland turbidity standards. Five c…

Abstract: Bensulfuron-methyl (BSM) is a widely applicable sulfonylurea herbicides. However, BSM remains in environment for extended period of time (over 100 days) and causes a series of environmental problems. In this study, BX2, a strain presents high BSM degradation abilities, was isolated from an activated sludge sample using a continuous enrichment method and determined to be a member of the genus Rhodococcus based on its physiobiochemical characteristics and 16S rRNA gene sequence. Strain BX2 degradated 92% BSM from an…

Abstract: Rhizobial strains that nodulate Vigna radiata (mungbean) were examined for their resistance to several stress conditions. One strain of mungbean rhizobia was selected to construct a collection of transposon mutants based on resistance to high temperature (40°C), alkalinity (pH 8.0) and several heavy metals. One mutant that is deficient in resistance to high temperature, alkalinity and zinc was selected to estimate the relation between resistance to stress conditions and symbiotic performance. Southern hybridization…

Abstract: In order to study domestic wastewater influence on secondary metabolite in thyme (Thymus vulgaris L.) under field condition, this experiment was carried out in Iran. In the field, 15 lysimeters having thyme (T. vulgaris L.) were used, where 1 to 5 lysimeters were irrigated by domestic wastewater and primary drainage water was accumulated. 6 to 9 lysimeters were irrigated by primary drainage water and then, were accumulated by secondary drainage water and we have irrigation 10, 11 and 12 lysimeters by secondary drai…

Abstract: Tolerance of the resting cysts of protozoa against gastric acid and proteases is a strategy for surviving in the digestive tract of animals. The present study aimed to examine the protection mechanism against HCl in resting cysts of Colpoda cucullus, which were surrounded by a cyst wall, which is composed of an outermost layer (ectocyst) and several inner layers (endocyst). In addition to water, paraformaldehyde, hydrogen peroxide (H2O2) and ethanol may diffuse across the cyst wall, because the cysts hardly showed…

Abstract: Siderophores, a high-affinity chelating agents for ferric iron, is one of the mechanisms by which biocontrol agents’ act in inhibiting the growth of phytopathogens. Bacillus subtilis QM3, a siderophore producer, is a potential biocontrol agent. In this study, a simple double-layered chrome azurol S agar (SD-CASA) plate assay was developed. We further studied optimal conditions for detection siderophore production by B. subtilis QM3 using various history cultures, pH value, amino acid, and different carbon-nitrogen…

Abstract: To study most prevalent wheat seed-borne mycoflora and its effect on the nutritional value, freshly harvested three and six months old stored wheat grains were collected from various parts of Pakistan. For isolation of seed-borne fungi, agar plate method was found more effective as compared to standard blotter method. Alternaria alternata was found to be the most prevalent fungus. Chemical analysis of seeds clearly showed a decrease in the carbohydrate, fats and ash contents of stored wheat grains as compared to th…

Abstract: Polymerase chain reaction (PCR) are rapid and simple means for the differentiation of members of Mycobacterium tuberculosis complex, especially Mycobacterium bovis and M. tuberculosis, where it is important to distinguish between zoonotic sources (cattle and unpasteurized dairy products) and human sources of tubercle disease. This study is aimed to evaluate the recent technique such as PCR and (BACTEC MGIT 960 TM system) for diagnosis of M. tuberculosis complex among cattle in Egypt. 1180 cattle were examined durin…

Abstract: 14-O-demethyledelboxine is one of the most important alkaloids in Consolida species. It is toxic and has many effects in body. In this article, we used dipole moment and nuclear magnetic resonance (NMR) to describe this molecule properties. Nanoparameters and chemical shift calculations, geometry optimization and energies have been performed with ab initio method at HF/Sto-3G, B3Lyp/6-31G and B3Lyp/6-31G* levels with magnetic properties of the gauge-including atomic orbital method. According to this study this mole…

Abstract: Pseudomonas aeruginosa is a commonly found nosocomial opportunistic pathogen that is characterized by its ability to form biofilm to protect itself from various external pressures. Biofilms are problematic in particular food industry sectors and in water distribution systems. In this study, we propose to explore the impact of UVC dose on biofilm production by P. aeruginosa strains. The biofilms densities were assessed by crystal violet assay. A relationship between UVC exposed dose and biofilm density was examined…

Abstract: According to the definition of probiotics by the Food and Agriculture Organization of the United Nations/World Health Organization, “probiotics are live microorganisms, which when administered in adequate amounts confer a health benefit on the host”. Microorganisms that are probiotics in humans include Enterococci, Bifidobacteria and lactic acid bacteria, such as Lactobacilli, Lactococci and Streptococci. This research was conducted to determine the presence of antibacterial effects among the probiotics isolated fr…

Abstract: Anthracnose disease is one of the major economic constraints to chilli production in tropical and subtropical regions. Ten isolates of Colletotrichum capsici causing chilli anthracnose were collected from 10 provinces in the northeast of Thailand. The isolates were evaluated for their morphological and cultural characteristics, pathogenic variability on chilli fruits and genetic characterization using random amplified polymorphic DNA (RAPD-PCR). Based on the morphological traits and cultural characteristics of the…

Abstract: This review focused on the worldwide contamination of foods and feeds with mycotoxins as a significant problem. Mycotoxins are secondary metabolites of molds that have adverse effects on humans, animals, and crops that result in illnesses and economic losses. Aflatoxins, ochratoxins, trichothecenes, zearelenone, fumonisins, tremorgenic toxins, and ergot alkaloids are the mycotoxins of greatest agro-economic importance. Factors influencing the presence of mycotoxins in foods or feeds include environmental conditions…

Abstract: Genetic diversity was studied for blast resistant and susceptible genotypes using 13 morphological characters. Plant height, days to flowering and maturity, panicle length, number of spikelet per panicle, number of filled grain per panicle, number of unfilled grain per panicle, 1000-grain weight and yield per hill were indicated as important contributors to genetic divergence in 14 rice genotypes. The first 3 principal components accounted for 78.72% of the total variation among resistant and susceptible rice genot…