Advanced Journal of Microbiology Research

Abstract: Nosema pernyi is the lethal pathogen of pebrine disease in Antheraea pernyi. We have developed a PCR-based method for detection of N. pernyi using specific primers. The primers were designed by the reported conserved regions of microsporidian SSU rRNA. When the genomic DNA of N. pernyi was used as the DNA template, the specific DNA sequences were amplified by PCR. It was observed that PCR diagnosis of N. pernyi using the three sets of primers provided increased specificity and sensitivity when compared with light m…

Abstract: Predicted highly expressed (PHX) genes were analyzed in five human pathogens with significant translation selection measured by within-group correspondence analysis (WCA). Functional analysis showed that in the five genomes PHX genes involved in protein synthesis, constitute the largest functional category, followed by categories of energy metabolism and protein fate. The gene encoding pyruvate kinase involved in glycolysis was PHX gene in most genomes except Bacillus anthracis Ames, which preferred strictly aerobi…

Abstract: RHIZOCTONIA SOLANI is one of the most important soil-borne fungal pathogens that attack the roots of plant and causes significant damage to different plants particularly to chickpeas (CICER ARIETINUM L.). The aim of this work is to study the antagonistic activity of different RHIZOBIUM strains against R. SOLANI in dual culture IN VITRO and under greenhouse conditions. The benefits of rhizobial inoculant in nitrogen fixation, phosphorous uptake and on plant growth promoting were demonstrated with 42 RHIZOBIUM strain…

Abstract: Soil reaction (pH) is important property soil for crop productions. Many chemicals as ameliorates are required to make soil productive. In organic agriculture, bio-ameliorates are prerequisite. Rhizobia are acid producers and Bradyrhizobia are alkali producers. Rhizobia and Bradyrhizobia are used as ameliorate to study their impact on soil reaction (pH) in laboratory conditions for 21 days. It was observed that Rhizobia decreased soil pH and Bradyrhizobia increased soil pH during incubation.[...]

Abstract: Polyhydroxyalkanoates (PHA) are the biodegradable polyesters produced by bacteria to overcome the environmental stress. Contaminated environments have a large number of bacteria which can accumulate PHA as their energy reserves. Molasses, oil/ghee and sewerage contaminated soils were selected for the screening of biodegradable plastic- producing bacterial strains. The selected contaminated environments showed great diversity of bacterial flora, PSEUDOMONAS (39.39%) being the most common. The 54 bacterial isolates w…

Abstract: Biodiesel is an alternative renewable fuel and its production rises rapidly. Microbial oils produced by oleaginous microorganisms can be used as a feedstock for biodiesel production so as to sustain the increasing demand for biodiesel and alleviate the competition with the food supply. This study investigated oil production by an oleaginous fungus, Mortierella sp., which was isolated from soils of Tamil Nadu in India. The flask culturing experiment shows that the optimal lipid production conditions were glucose as…

Abstract: Avian infectious laryngotracheitis (ILT) is a severe clinical respiratory disease of chickens and causes the clinical symptoms of difficulty in breathing and bloody coughing and as if involves laying hens affect the egg production. Two different regions of the infected cell protein 4 (ICP4) gene of infectious laryngotrucheitis virus (ILTV) were amplified and sequenced for characterization of field isolates and tissue culture-origin (TCO) and chicken embryo-origin (CEO) vaccine strains. Phylogenetic analysis of the…

Abstract: In our previous studies, Rat-1 cells treated with Vibrio parahaemolyticus thermostable direct hemolysin (TDH) developed morphological changes including shrinkage and rounding of the cells. Such alterations suggest that TDH stimulates reorganization of the cellular cytoskeleton. In the current study, the effects of TDH on actin cytoskeleton, chromatin, and caspase-3 were evaluated. Rat-1 cells treated with TDH 5 µg/ml showed redistribution of actin with loss of stress fibers, a floccular staining pattern, cellular m…

Abstract: The aim of the study was to isolate the exopolysaccharides (EPS) from the active biofilm bacterium Bacillus cereus GU 812900 which was isolated from the stainless steel test panel and deployed in the Vellar estuary for a period of about four weeks. The deployed stainless steel test panels when analyzed revealed a direct relationship between the rate of corrosion and the concentration of EPS that is, the rate of corrosion increased with an increase in the concentration of EPS and the period of immersion. Growth and…

Abstract: Lentil (Lens culnaris Medik.) is an invaluable source of protein for the vast majority of Ethiopian people. It also maintains soil fertility through symbiotic nitrogen-fixation in association with Rhizobium leguminosarum bv. viciae. The aim of this study was selecting symbiotically efficient native lentil nodulating rhizobia endowed with different plant growth-promoting (PGP) characteristics. Hence, 30 lentil nodulating rhizobial isolates were isolated from soils collected from different farmer fields found in Cent…

Abstract: GB virus C (GBV-C)/hepatitis G virus (HGV) by infecting and encourage proliferative process of the mononuclear cells, may implicate in lymphomagenesis including non-Hodgkin’s lymphoma (NHL). Therefore, in this study the prevalence of GBV-C/HGV and HBV infections were evaluated in patients with NHL and controls. In a cross sectional study, blood samples were collected from 70 patients with NHL and 100 healthy controls. The infective markers of GBV-C/HGV and HBV viruses were evaluated in both studied groups by enzyme…

Abstract: Dried meat (Tinko) samples obtained from the Oja-Oba market in Ilorin metropolis during the rainy season were investigated for their microbial flora and the sensitivity of the bacterial isolates to different chemotherapeutic antibiotics under two different storage conditions (Cupboard and Refrigerator) for a period of five weeks revealed that the moisture content of dried meat samples stored in the cupboard increased from 35 to 65%, while those in the refrigerator had an initial increase from 50 to 55% for the firs…

Abstract: The aim of this study was to screen the presence of antimicrobial activities in the marine mollusc Thais tissoti and Babylonia spirata. In this study, the human bacterial pathogen Klebsiella pneumonia (10.02 ± 0.11 mm) and fungal pathogen Aspergillus niger (12.09 ± 0.06 mm) showed most sensitive to ethyl acetate extracts of T. tissoti. In B. spirata extracts, bacterial pathogen Proteus mirabilis (7.02 ± 0.04 mm) and fungal pathogen Candida albicans (8.13 ± 0.15* mm) exhibit high zone of inhibition against n-butanol…

Abstract: The antifungal activity of the Polyhexamethylene-guanidine hydrochloride (PHMGH)-based disinfectant against fungi isolated from papaya fruit was evaluated. The aim of this work was to show that the PHMGH can be used as a disinfectant for papaya preservation. Thirty one strains of fungi were isolated as Mucor sp., Botrytis sp., Penicillium sp., Geotrichum sp., Aspergillus sp. and Colletotrichum sp. Mucor sp. was the most isolated with a frequency of 52.77% followed by Botrytis (47.22%), Aspergillus and Penicillium (…

Abstract: Bioremediation is a simple and effective technology for metal extraction from low-grade contaminated soils and mineral concentrates. Metal remove from sulfide minerals is based on the activity of mesophilic and chemolithotrophic bacteria, mainly ACIDITHIOBACILLUS FERROOXIDANS which convert insoluble metal sulfides into soluble metal sulfates. In this study bioremediation experiments carried out in 1 L Erlenmeyer flasks containing 300 ml basal medium of A. FERROOXIDANS and 5% (w/v) PbS with 45 and 75 meshes and also…