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I. B. Omamor, E. I Eziashiand A. A. Adekunle
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Dewang Deng, Allan Zipf, Y. Tilahun, G.C.Sharma, J. Jenkins  and K. Lawrence
Abstract: A new nematode extraction technique was established, which is based on an iso-osmotic density- gradient medium (OptiPrepTM). This technique resulted in significantly higher numbers of clean eggs and vermiform nematodes that retain higher viability (48.6%) than samples processed with the sucrose method (28.7%). Nematodes survived exposure to OptiPrepTM for 22 hours without significant mortality whereas all nematodes died in the sucrose medium. OptiPrepTM provided a suitable, non-toxic alternative to the traditional density gradient material for the isolation of nematodes. This technique is convenient and relatively simple, with the added benefit of yielding cleaner samples compared to traditional isolation techniques.[...] Read More.
Keywords: nematode extraction, density gradient, nematology.
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Mutlag Al.Otaibi* and Hassan El.Demerdash
Abstract: Three essential oils, namely thyme, marjoram and sage, were added to concentrated yoghurt (labneh) at concentrations of 0.2, 0.5 and 1.0 parts per million (ppm). Subsequently, the chemical, microbiological and organoleptic properties of freshly prepared labneh and of the labneh stored at 5˚C ± 1 for up to 21 days were determined. Addition of essential oils affected the pH, soluble nitrogen -to-total nitrogen, total volatile fatty acid and acetaldehyde values of the prepared labneh. On the other hand, total solids and fat-to-dry matter values were only slightly affected. Total viable counts, as well as counts of Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus in the treated labneh increased and reached a maximum after 7 days of storage where after it decreased until the end of the storage period. Yeasts and moulds, coliform bacteria and spore-forming bacteria were not detected in the treated labneh. Of the different treated labneh, labneh containing 0.2 ppm thyme, marjoram or sage oils were organoleptically the most acceptable, and it had a good body and texture that was similar to that of the untreated control. From the results of this study, it can be concluded that 0.2 ppm of thyme, marjoram or sage can be used in order to increase the shelf life of labneh for up to 21days.[...] Read More.
Keywords: Labneh, essential oils, shelf life, chemical properties, microbiological properties
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Echeonwu G. O. N*, Iroegbu C. U, Echeonwu B. C, Ngene A, Nwosuh C. I, Joannis T. M and Ndako J.
Abstract: Cassava granules coated with Newcastle disease virus (NDV) strain V4-UPM was used to vaccinate free-range chickens in their natural habitat. Immune response, vaccine virus excretion and the efficacy of the food vaccine were assessed by standard methods. Results show that out of 218 chickens given initial food vaccine in the four locations, 138 (63.3%) produced detectable HI antibody while 202 (92.7%) had titres < 3.0. However, only 16 (7.3%) attained log2 3.0 with GMT of 3.2. This was made up of Nchara- Akanu 7(12.7%), Vandekya 0(0.0%), Fadan Karshi 1(1.7%), and Turu 8(15.1%) with GMTs of 3.3, 2.9, 3.0, and 3.6 in that order. Following the administration of a booster dose of vaccine on 194 birds in the same flocks, 170(87.6%) sero-converted with 118(60.8%) attaining log2 3.0 and GMT of 9.7. Chickens attaining HI titres up to log2 3.0 from the locations were as follows, Nchara-Akanu 26(51.0%), Vandekya 22 (51.2%), Fadan Karshi 28(53.8%), and Turu 33(68.8%) with GMTs of 12.8, 7.5, 7.0, and 12.6 respectively. Vaccinated birds excreted infective vaccine virus. Out of 55 buyback chickens challenged, 15(27.3%) died while 40(72.7%) survived. Twenty two (22) out of 24 (91.7%) unvaccinated birds challenged died and only 2(8.3%) survived. It is therefore concluded that cassava granules could be good carrier for food-borne ND vaccine delivery to village chickens in Nigeria.[...] Read More.
Keywords: Cassava, V4-UPM virus, village chickens, Newcastle disease.
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文章
Ng Hong Jing, Fatin Hanani Sulaiman, Roswanira Ab. Wahab, Rolando V. Pakinging Jr. , Noor Aini Abdul Rashid and Fahrul Huyop*
Abstract: The bacterial isolate HJ1, which was identified as a Methylobacterium sp., grew on 2, 2-dichloropropionic acid as the sole carbon source and produced a 2-haloalkanoic acid hydrolytic dehalogenase. This non-stereospecific dehalogenase E (DehE) catalysed the hydrolytic dechlorination of 2, 2-dichloropropionic acid and D, L-2-chloropropionic acid to produce pyruvate and lactate, respectively. The enzyme was purified to homogeneity and characterized. The molecular weight was 36 kDa by SDS-polyacrylamide gel electrophoresis and 72 kDa by gel filtration, suggesting that the enzyme is a protein dimer. The purified enzyme was only inhibited by HgSO4 and was non-stereospecific to haloalkanoic acids. The Km value for the hydrolysis of 2, 2- dichloropropionic acid was 0.25 mM. The enzyme removes chloride present on the -position, but not on the -position, of a number 2–carbon alkanoic acids.[...] Read More.
Keywords: haloalkanoic acid, dichloropropionate, 2, 2-dichloropropionic acid.
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Salem, A. Mahfouz F. M. Shaieb and A.E.K Elzen
Abstract: Drinking water systems supplied by untreated groundwater were examined to determine whether coliform or heterotrophic plate count bacteria are capable of growing. Filterable bacteria were present in 42% of the 46 groundwater sources examined by using nonselective media (R2A and full strength m-HPC agars). Pseudomonads were the most frequently identified group of filterable bacteria detected. Flavobacterium, Alcaligenes, Acinetobacter and Achromobacter isolates were also identified. Total coliforms were recovered only from six samples taken from wells near the wastewater lagoon, following filtration through 0.45-µm-pore-size membrane filters by using selective M- Endo LES agar or mT7 agar. In addition, none of the isolates identified from nonselective media were coliforms. Similarly, neither total coliforms nor specifically Escherichia coli were detected in these filtrates when Colilert P/A medium was used[...] Read More.
Keywords:  ground water, contamination, Flavobacterium, Alcaligenes, Acinetobacter, Achromobacterand E. coli
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G. A. Ayoola*, F. M. Lawore, T. Adelowotan, I. E. Aibinu, E. Adenipekun, H. A. B. Coker and T. O. Odugbemi
Abstract: Steam distillation of the dry flower buds of Syzigium aromaticum (clove) yielded 7% (w/w) of the pure light yellow oil. Gas chromatography-mass spectrometry (GC-MS) analysis of the oil revealed that the components were eugenol, caryophyllene, eugenol acetate and alpha-humelene, with eugenol being the main component. The antimicrobial sensitivity of the volatile oil against some Gram-negative bacteria (Escherichia coli ATCC 35218, Escherichia coli, Klebsiella pneumoniae, Salmonella paratyphi, Citrobacter spp. and Enterobacter cloacae), a Gram-positive bacterium (Staphylococcus aureus ATCC 25923), and a fungus (Candida albicans) showed a broad spectrum of activity. The minimum inhibitory concentration (MIC) was determined for each organism as 2.4, 1.6, 0.27, 0.016, 0.23, 1.63, 0.73 and 0.067 mg/ml for S. aureus ATTC 25923, E. cloacae, S. paratyphi, K. pneumoniae, E. coli ATTC 35218, E. coli, Citrobacter spp. and C. albicans, respectively. Antioxidant screening of clove oil with 2,2-diphenyl-picryl-hydrazyl radical (DPPH) was positive, indicating the presence of free radical scavenging molecules which can be attributed to the presence of eugenol, a phenolic compound.[...] Read More.
Keywords:  Syzigium aromaticum, clove oil, antimicrobial, antifungal, Gram-negative bacteria, Gram-positive bacteria, Gas chromatography, mass spectrometry.
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Neveen S. M. Soliman and Salwa A. Aly*
Abstract: This study aims at identifying the diversity and abundance of yeast associated with Egyptian Karish cheese, employing comparison between conventional laboratory techniques and API20 kits techniques in yeast identification. A total of one hundred samples (fifty each) of Egyptian raw and pasteurized Karish cheese milk were randomly collected from farmers and markets in Cairo and Giza Districts. The occurrence of yeast in raw and pasteurized Karish cheese milk were 100 and 38% with a mean value of 7 ± 1.1 l and 1 ± 0.31 log10 cfu g−1, respectively. Yeast strains isolated from both raw and pasteurized karish cheese samples were identified and characterized using both conventional methods and API 20 C AUX as a commercial identification system. The most prevalent isolates belonged to Trichosporon cutaneum (25%), Candida catenulata (23%), Yarrowia lipolytica (13%), Debaryomyces hansenii (13%), Kluyveromyces lactis (6%), Geotrichum candidum (7%), Candida zeylanoides (5%), Candida lambica (3%), Candida albicans (2%), Cryptococcus formans (1%), Rhodotorula glabrata (1%) and Saccharomyces cerevisiae (1%). There was no significant difference (P 0.05) between the conventional method and the API20 kits test. However, the results of this study reveal that API 20 kits are simple, highly useful and are commercially available kits that considerably shorten the time required for the identification of yeast in cheese.[...] Read More.
Keywords: Yeast identification, Karish cheese, API 20 kits.
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Hattem H. E.*, Manal A. Naeim, Hanaa S. Sakr and Elham H. Abouel-Einin
Abstract: The present study was carried out to investigate the effect of different thermal treatments on the composition and chemical properties of camel milk. The rennet clotting time of camel milk was also investigated. Camel milk samples were thermal treated at 63, 80 and 90°C for 30 min and 72°C for 15 s, whereas raw milk sample was served as a control. We found that the fat content was not affected by the applied treatments (3.2±0.189%), but the protein contents average ± SD values were found to be 3.2±0.148, 3.4±0.136, 3.4±0.149, 3.3±0.049 and 3.1±0.157%, respectively. The ash contents were also affected by the thermal treatments and their average ± SD values were 0.70±0.065, 0.71±0.056, 0.73±0.052, 0.71±0.088 and 0.68±0.096%, respectively. The thermal treatments affected also the total solids in the samples; 10.0±1.168, 10.10±1.057, 10.16±1.089, 10.05±1.055 and 9.9±1.189%, respectively. The non protein nitrogen (NPN), non casein nitrogen (NCN) and whey protein nitrogen (WPN) gradually decreased as thermal treatments were increased but casein number and the percentage of denaturation were increased. Rennet clotting time in the presence of different concentrations of CaCl2 (0 to 20 mg /100 ml) was found to be increasing by raising the temperature. However, increasing the amount of calcium chloride was found to be decreasing the rennet clotting time at all thermal treatment. Incubation of milk with yoghurt culture at 40°C for 12 h revealed a significant increase to the acidity level and a substantial decrease in the pH level at all the applied thermal treatments.[...] Read More.
Keywords: Camel milk, heat treatments, chemical composition, some properties.
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Idise, Okiemute Emmanuel
Abstract: Wine was produced at 1:4 (must: sugar) from coconut (Cocos nucifera) using natural yeast (Recipe A), natural yeast augmented with granulated sugar (Recipe B), natural yeast augmented with Baker’s yeast, granulated sugar (Recipe C), a control consisting of granulated sugar and Baker’s yeast (Recipe D). On fermentation for 120 h, pH values were 4.68±0.191, 4±0.029, 4.08±0.023, 3.65±0.058, temperature values (°C) were 28.75±0.61,  27.75±0.26, 28.25±0.43,  27.5±0.58,  specific gravity values were 1.003±0.0006, 1.012±0.00171,   1.001±0.0006,   0.99±0.0023,   optical   density   values   were   0.6918±0.019,     0.715±0.017, 0.774±0.0023,    0.752±0.005,    %   alcohol   (v/v)   values   were    1.359±0.002,    1.371±0.006,     1.357±0.007, 1.354±0.008,% titratable acidity values were 0.16±0.001, 0.809±0.044, 0.302±0.002, 0.382±0.015, Rf values were 4.7±0.017, 4.9±0.058, 4.25±0.052, 4.95±0.058 and total aerobic counts were 7.308±0.036, 7.183±0.067, 6.72±0.046, 7.175±0.014 for Recipes A to D respectively. Malo-lactic fermentation after 48h was evident. Taste testing showed very little differences in wines from Recipes A to C. Statistical analyses at 95% confidence level showed no significant differences in coconut wine produced with different recipes for the tested parameters. The wine from the control had similar taste and characteristics with natural palm wine. Coconut wine could thus be produced for immediate consumption within 48 h using the Recipes A - C.[...] Read More.
Keywords: Coconut, flora, fermentation, sugar, wine, flavor, yeast.
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