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Cyril-Olutayo Christianah Mojisola*, Elujoba Adebolu Anthony and Durosinmi Muheez Alani
Abstract: The antisickling properties of fermented mixture of dried unripe fruit pulp of Carica papaya and dried Sorghum bicolor leaves, mixed in equal proportions in distilled water, was carried out using sodium metabisulphite sickled red blood cells and the result presented. Equal weight of dried C. papaya fruit pulp and S. bicolor leaves were fermented together in distilled water at room temperature and the aque-ous extract obtained and used for antisickling assays. The extract gotten from the materials incubated for 5 days indicated as SP5, was found to have the highest antisickling properties with 93% inhibitory and 84% reversal activities. The concentration of the day 5 extract was further varied. 0.2 ml was found to be the optimum volume of the test extracts.[...] Read More.
Keywords: Antisickling, reversal, inhibitory, Carica papaya, Sorghum bicolor, sickle cell anaemia.
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Hanaa H. Abd El-Baky*, Farouk K. El Baz and Gamal S. El-Baroty
Abstract: This study illustrates the process of enhancing phenolics synthesis in Spirulina maxima grown in Zarrouk’s medium supplemented with sodium nitrate (NaNO3) and or phenylalanine (L-PA), attaining highest production obtained in medium containing 3.77 g.L-1 NaNO3 and 100 mgL -1 L-PA. HPLC profile showed the presence of phenolic acids and flavonoids predominantly with gallate, chlorogenate, cinnamate, pinostrobate and p-OH- benzoates. The protective action of Spirulina phenolic compounds ( SPC) against CCl4-induced in vitro hepato-toxicity symptoms like microsomal lipid peroxidation and hydroxyl radical formation was studied. SPC exhibited antioxidant effects on DPPH radical scavenging with IC50 values ranging from 23.22 to 35.62 mgmL-1 and inhibit CCl4 induced lipid peroxidation in hepato- microsoms model, in dose-depended manner. Their protective potential was comparable to that of standard phenolic antioxidants such as BHT, BHA and – tocopherol (IC50 values ranged from 13.22 to 23.62 mgmL-1).[...] Read More.
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F. A. Fehintola
Abstract: The overall goal of this study is to reduce morbidity and mortality ascribable to bacterial infections by encouraging rational use of antibiotics. Antibiotics use prior to and prescriptions of antibiotics by the attending physicians were evaluated in a group of patients attending a secondary health facility. A quasi-exit interview was conducted using a structured questionnaire. The major presenting symptoms were sought from patients and/or parents and/or guardians; drug history was taken and doctors’ prescriptions were copied onto an already prepared format. All data were entered into EPI-INFO version 6 for analyses. The mean age of patients who were enrolled was 14 ± 16.96 [range: 0.08-78 years] but males patients were statistically younger than females: respectively 9.94 ± 15.48 years (0.08-78 years) and 18.43 ±17.10 years (range: 0.08 – 70 years); F: 122 P< 0.00. Pre-hospital use of antibiotics was documented in about a third of all the patients and cotrimoxazole was the most commonly used antibiotics accounting for 68.5% of antibiotics use in this group patients. Antibiotics were contained in more than half of all the prescriptions and erythromycin and cephalosporin were antibiotics of choice. This is contrary to the previous findings in the same area of study but different health facility. There is need for formulation of appropriate drug policy and establishment of continuing medical education for doctors as well as public enlightenment programmes on rational use of antibiotics.[...] Read More.
Keywords: Antibiotics Nigeria prescription cotrimoxazole use pre-hospital.
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Namrata Singh,* Aseem Bhatnagar
Abstract: 99mTc-INH of high labeling efficiency and stability has been developed using indirect method. In vitro studies and animal experiments indicated its advantages as a specific tuberculosis imaging agent. The objective of this study was to establish the efficacy of 99mTc-INH in humans with sensitive as well as resistant tuberculosis by conducting a phase I clinical trial. The biodistribution studies were done in normal subjects and phase I clinical trial was conducted in 20 patients. Whole body scan and spots were acquired at 1 and 4 h. Angiography, blood pool and 24 h spot images of the lesion bearing areas were also acquired. The biodistribution suggested absence of in vivo breakdown of radiotracer, with main excretory pathways being hepatobiliary and renal. The biodistribution of 99mTc-INH was similar to the unlabeled INH reported earlier. Out of 20 patients, 13 patients with sensitive tubercular lesions in the lungs or bone and 2 patients with resistant tubercular lesion in lungs concentrated the 99mTc-INH while in the other 5 cases with old healed lesions no concentration of 99mTc-INH was observed in scintigraphy. An unsuspected bony lesion was discovered in a patient with known pulmonary disease. Bone lesions were visualized within 1 h while pulmonary lesions accumulated 99mTc-INH very slowly with time and 24 h acquisition appeared essential for the diagnostic interpretation. No adverse reaction was observed in the patients post injection. 99mTc-INH developed is safe for human use and has potential to qualify as a specific tuberculosis imaging radiopharmaceutical.[...] Read More.
Keywords: Isoniazid (INH), 99mTc, scintigraphy, clinical trials, radiopharmaceutical.
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K.  Warda, M. Markouk*, K. Bekkouche, M. Larhsini A. Abbad, A. Romane and M. Bouskraoui
Abstract: In Morocco, most people particularly in rural areas use medicinal plants to treat diseases. In order to find new antipneumococcal extracts, an ethnobotanical survey has been conducted in different regions of Marrakesh (south of Morocco). Four plants often cited by traditional practitioners (Marrubium vulgare, Thymus pallidus, Eryngium ilicifolium and Lavandula stoechas) are tested against Streptococcus pneumoniae responsible for pharyngitis, rhinitis, otitis and sinusitis infections. Aqueous and methanol extracts have been prepared and tested on S. pneumoniae collected in four regions of Marrakesh. A significant activity has been observed with methanol extracts of three plants; M. vulgare, T. pallidus and L. stoechas (MIC= 256 µg/ml).[...] Read More.
Keywords: Streptococcus pneumoniae, antibacterial activity, medicinal plants, phytochemical screening.
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G. Arunachalam*, N. Subramanian, G. P. Pazhani and V. Ravichandran
Abstract: The methanolic extract of leaves of Eclipta prostrata Linn was investigated for anti-inflammatory activity in albino Wistar rats. The methanolic extract administered by the oral route at a concentration of 100 and 200 mgkg-1 showed the significant dose dependent anti-inflammatory activity in carrageenin and egg white induced hind paw oedema in rats. Anti-inflammatory activity of the tested extract was comparable with that of the standard drug indomethacin (10 mgkg-1) and cyproheptadine (8 mgkg-1) . The results lend support to the traditional use of E. prostrata in the treatment of inflammatory diseases.[...] Read More.
Keywords: Eclipta prostrate, methanolic extract, albino Wistar rats, anti-inflammatory activity.
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B. R. Ghalem* and B. Mohamed
Abstract: The essential oil from the gum of Pistacia atlantica Desf. grown in Algeria was obtained by the hydrodistillation method, and its antimicrobial activities against the growth of clinical isolates of Staphylococcus aureus, Escherichia coli and Streptococcus pyogenes were evaluated using three different methods; agar disc diffusion and dilution broth methods and minimum inhibitory concentration (MIC) which was subsequently, determined. The results of the study revealed that essential oil resin of P. atlantica has antimicrobial activity against gram-positive and -negative bacteria which are resistant to commonly used antimicrobial agents, and they were considerably dependent on concentration.[...] Read More.
Keywords: Gum of Pistacia atlantica Desf., essential oil, antimicrobial activities, Staphylococcus aureus, Escherichia coli, Streptococcus pyogenes.
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Gamal Khalafalla Mohamed Ali
Abstract: This paper presents a comprehensive evaluation of a country where a revolving drug fund (RDF) has lasted for fifteen years and serves more than three million patients annually, with more than SDG 2.8 million (Sudanese Pound) (US$11.1 million) annual turn over. Regardless of the fact that the supply of medicines and improvement in public health facilities utilization are among the main objectives of user fees policy, there is little information on the effect of RDF on accessibility of essential medicines and its impact on the utilization of public health services where RDF schemes have been introduced. We measured the percentage of prescribed medicines dispensed to patients in selected health facilities (both RDF and non-RDF), the availability of essential medicines in a twelve month period in order to determine whether the cost of the medicines is a barrier to utilization of public health services with RDF scheme in Khartoum State (KS). Structured interviews with users (186), personal observations, and archival as well as statistical records were used to capture data of interest relevant to the study objective. The average availability rate of key items was greater (93%) in the RDF facilities compared to 86% in non-RDF facilities. RDF records also showed that the availability of medicines in the RDF health facilities ranged from 95 to 100% in the twelve months period studied. Data from the household survey, demonstrated that over a third (36%) of respondents did not consult public health facilities when a member of a household was ill two weeks prior to the date of interview. Of note, only 9% of them said that this was because of unavailability of medicines. This study suggests that the RDF scheme adopted by KS made essential medicines available at its health facilities and increased health services utilization compared to those without RDF scheme. Therefore, sustained availability of low cost medicines near where people live that benefit previously disadvantaged poor population, particularly the vulnerable rural groups is achievable through RDF.[...] Read More.
Keywords: RDF, accessibility, availability, affordability.
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H.N. Wanyika, P.G. Kareru*, J.M. Keriko, A.N. Gachanja, G.M. Kenji, N.J Mukiira
Abstract: The contact toxicity of some selected fixed plant oils and stabilized natural pyrethrum (Chrysanthemum cinerariaefolium) blends against adult maize weevils (Sitophilus zeamais) were investigated. Natural pyrethrum extract was stabilized against ultraviolet (UV) light by blending with fixed oils extracted from Azadirachta indica A. Juss (neem tree), Thevetia peruviana (yellow oleander) and Gossypium hirsutum L. (cotton) seeds. Cottonseed oil had the highest stabilization effect on the pyrethrum blend exposed to UV light of 366 nm. The results indicated that the natural pyrethrum extract blended with cottonseed oil was the most potent against maize weevils and that the potency was concentration-time dependent. Cottonseed and neem seed oils enhanced the stabilization of the natural pyrethrum insecticide.[...] Read More.
Keywords: Contact toxicity, maize weevils, ultraviolet light, natural pyrethrum, cottonseed oil.
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文章
P. Thirupathy Kumaresan*, A. Saravanan
Abstract: Anticonvulsant activity for Morinda tinctoria was evaluated in albino mice of either sex at 3 different dose levels (200, 400 and 600 mg/kg ip) by MES and chemical methods. The extract showed significant (p[...] Read More.
Keywords: Morinda tinctoria, anticonvulsant, PTZ, MES.
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