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Kacou-N’douba, A., *, Kazali, A.,  Koffi, K.S., Ekaza, E., Kouablan, A., Kangah, T., Okpo, S., Elogne-Kouamé, C. and Dosso, M.,
Abstract: The worldwide spread of community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) skin infections is becoming an emerging problem. These bacteria can produce virulence factors. The objectives of this study were to determine the rate of MRSA and frequency of mecA gene by PCR and detect genes eta and etb encoding exfoliatin A and B. This is a prospective study focused on school children of 5 to 15 years with a cutaneous infection from October 2007 to June 2008. The skin samples taken were allowed to isolate strains of S. aureus by standard bacteriological methods. Susceptibility testing was performed to detect strains of MRSA with cefoxitin disk. Detection of mecA, eta and etb genes was performed using PCR. The rate of MRSA was 14.8%. The mecA gene was present in 2 strains of S. aureus (3.7%). The genes encoding the exfoliatin were detected in 5.6%.[...] Read More.
Keywords: Community-acquired skin infection, MRSA, exfoliatin A and B.
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Mukesh Sharma* and Meenakshi Sharma
Abstract: Culture media significantly affected the growth, sporulation and conidial discharge of any microorganisms. The present investigation was conducted to examine the effect of broth and agar media on the mycelial growth and fungal sporulation of six species belonging to five genera of fungi. Soil fungal dermatophytes (Trichophyton mentagrophytes, Microsporum gypseum and Microsporum fulvum) were isolated from soil using hair-baiting technique and clinical isolates (Epidermophyton floccosum, Trichophyton schoenleinii and Scopulariopsis sp.) were procured from superficial skin of dermatomycoses patients under the supervision of dermatologists. Variation in mycelial growth and fungal sporulation was observed with media tested. Colony radial growth and sporulation of soil fungi were optimal on Sabouraud’s dextrose medium (SDM) followed by Potato dextrose medium (PDM) and Richard medium (RM). For clinical isolates, the suitable broth media were Potato dextrose medium (PDM) and Yeast extract medium (YEM). Among agar media studied, Sabouraud’s dextrose agar (SDA) and Potato dextrose agar (PDA) media were found most favored for both soil and clinical isolates.[...] Read More.
Keywords: Clinical isolates, culture media, mycelial growth, soil dermatophytes, sporulation.
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K. R. Sanjay*, M. N. Nagendra Prasad and G. S. Vijaykumar
Abstract: Isolation and identification of the drug resistant gram negative bacilli bacteria were carried out from post operative wound infections. Out of 214 samples collected, 167 showed bacterial growth and gram negative bacilli (GNB) growth was observed in 121 samples. In the present study E. coli was found to be the predominant agent isolated from wound infections (37.3%), followed by Pseudomonas (20.9%), Klebsiella (17.2%), Acinetobacter (14.2%) and other agents were less common. It was found that out of 121 gram negative bacilli isolates, 28 were found to be extended spectrum beta lactamase (ESBL) producers and 16 were mettallo beta lactamase (MBL) producers. Out of 52 isolates tested for minimum inhibitory concentration (MIC), 7 isolates showed MIC of 8 µg/ml, 42 isolates showed MIC of 0.5 µg/ml and other isolates showed intermediate concentration. All the ESBL and MBL positive isolates showed phantom zone in E-test.[...] Read More.
Keywords: Antibiotic resistance, post operative wound infection, beta lactamase, minimum inhibitory concentration.
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Ajayi Adedayo Olajide
Abstract: Water samples were collected from wells, bore holes and some streams at various locations within Akungba-Akoko, Ondo state. Pour plate technique was used for the estimation of Total bacterial count and Coliforms count of the water sources. Mean heterotrophic bacterial counts (cfu.ml) ranged from 1.0 x 104 in A.A.U.A storage tank to 122 x 104 in Oroke well. Total coliforms counts (cfu/ml) ranged from 0.01 x 104 in A.A.U.A storage tank to 36 x 104 and 38 x 104 in Oroke well and stream, respectively. Microorganisms isolated from the different water sources include Escherichia coli, Klebsiella spp, Bacillus spp, Bacillus cereus, Proteus spp, Streptococcus spp, Pseudomonas spp., Flavobacterium spp., Streptococcus feacalis, Pseudomonas aeroginosa, and Staphylococcus aureus. This study thus revealed that some indicator and pathogenic group of organisms like E. coli, S. feacalis and B. cereus constituting 13.34% each of the total number of microorganism isolated were largely encountered in the water sources from this area, hence, there is need for proper monitoring of the water sources to avert outbreak of epidemic disease. The pH of water sources ranged from pH 5.52 and 5.87 in Ilale borehole 2 and Ilale well respectively to pH 7.67 in Igbelu well. The temperature recorded range from 23 - 28°C for the sample sources. The wide variation in Physico-chemical parameters like pH from the neutral point signifies presence of contaminants that may favour the spread of some aetiologic agents of diseases that could be controlled through proper health delivery systems.[...] Read More.
Keywords: Akungba- Akoko, microbiological, Ondo state, ground water, surface water.
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Anthony, A. Adegoke *, Adebayo-Tayo, C. Bukola , Inyang, U. Comfort, Aiyegoro, A. Olayinka and Komolafe, O. Amos
Abstract: The microbiological (epidemiological), proximate and mineral element composition of the different species of snails (Achatina fulica, Limcolaria sp. and Helix pomatia) obtained from three different market in Uyo, Akwa Ibom state were investigated. Total bacterial count ranged from 1.00 - 1.50 x 108 cfu/g, Coliform count ranged from 1.68 - 2.20 x 107 cfu/g, Salmonella/Shigella count ranged from 5.2-8.2 x107 cfu/g, lactic acid bacteria count ranged from 1.03 - 1.30 x 108 cfu/g and fungi count ranged from 7.3 x 107 to 1.00 x 108 cfu/g. The organisms isolated were Bacillus subtilis, Staphylococcus aureus, Lactobacillus spp., Escherichia coli, Micrococcus luteus and Bacillus cereus while the fungal isolates were Aspergillus terrus, Aspergillus fumigatus, Absidia sp., Fusarium oxysporum, Eurotium sp. and Aspergillus flavus. The results showed that Helix pomata have the highest microbial load of 2.20 x 108 cfu/g. The proximate analysis showed that African giant snail (Achatina fulica) was nutritionally richer than the other snails. Mineral determination also showed that African giant snail had the highest amount of minerals. It was generally observed that snails though nutritionally rich are reservoirs of pathogenic microorganisms which are of public health importance.[...] Read More.
Keywords: Microbiological, proximate, mineral composition, coliform, Helix pomata
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Pradeep Parihar*, Leena Parihar and Achaleshwar Bohra
Abstract: The main objective of this research work is to screen various unexploited plants for their antimicrobial activity as these unexploited or pteridophytic plants are being used ethanomedicinally but, very little work has been done on antimicrobial aspects. So, to explore the efficacy of these plants, the following research has been carried out. Bacterial strains of Agrobacterium tumefaciens, Escherichia coli, Salmonella arizonae, Salmonella typhi and Staphylococcus aureus were procured from the Institute of Microbial Technology (IMTECH), Chandigarh and the aqueous and alcoholic leaves extract of twelve important pteridophytic plants were prepared and tested for their antimicrobial activity against the bacteria selected by Disc diffusion method as suggested by Bauer et al. (1966). It has been observed that, nearly all the leaves extracts have shown inhibitory effect against the bacterial strains selected and some of the extracts were more competent than the selected antibiotic. Our findings provide the novel insights with regards to antimicrobial agents and these could be further enhanced through in vivo studies and isolation and characterization of active constituents for human health. In the present scenario, the use of herbs and herbal medicine is at its peak and majority of researchers are screening higher plants for the same but, very few researchers are considering the lower plants for their antimicrobial potential. Since, these pteridophytic plants are considered to be the disease free plants and are being used ethanobotanically by various tribal communities. These plants are further screened for their in vivo potential as well as for their drug properties.[...] Read More.
Keywords: Antimicrobial activity, pteridophytic plants, leaves extracts, bacteria.
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Mercy Aparna L. Lingala, Aparna Srikantam*, Suman Jain, K. V. S. M. Rao and P. V. Ranganadha Rao
Abstract: Present study aims to study the geographical profile of rpoB mutations of Mycobacterium tuberculosis clinical isolates and their correlation with the therapeutic outcome. All the isolates were tested for drug susceptibility testing on Lowenstein-Jensen medium and PCR and DNA sequencing for rpoB gene mutations. Fifty two out of 101(52%) isolates were pan susceptible of which 79% were associated with cure. Thirty five isolates (35%) showed a combined resistance to isoniazid and rifampicin, out of which 63% were associated with treatment failure. Majority (94%) of the isolates from cured patients showed wild type of rpoB sequence; where as 77% of patients who failed the treatment were associated with mutations. Twenty eight out of 38(74%), rifampicin resistant isolates showed commonly occurring mutations such as 531, 526 and 516. Multiple silent mutations between the codons 145 - 184 (out side the hot spot region) are being reported for the first time in this study. Information on the geographical profile of rpoB mutations in M. tuberculosis may therefore facilitate for an improved diagnosis of rifampicin resistance, by increasing the efficacy of the gene sequencing based tests.[...] Read More.
Keywords: DNA sequencing, LJ culture, Mycobacterium tuberculosis, rpoB mutations.
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E. U. Umeh* and B. I. Umeakanne
Abstract: To assess the association between candida and HIV infections in women from different socio-economic backgrounds, vaginal swabs were collected from 510 women (aged 9 - 83 years) attending secondary healthcare units in southern part of Benue State, Nigeria. CANDIDA ALBICANS was identified by microscopy. Candida infection rate was higher among HIV-infected women (88.8%, n = 116) than among HIV negative subjects (58.6%, n = 394) (Χ2 = 36.077, p < 0.05; odds ratio = 5.59, 95% confidence interval (CI) = 3.03 - 10.297). Candida (yeast) infection was significantly associated with HIV infection in pregnant subjects (r = 0.504, p < 0.05), married (r = .290, p < 0.05), and unmarried women (r = 0.259, p < 0.05); in married women (and also unmarried women) vaginal yeast infection was significantly correlated/associated with HIV infection (p < 0.05). In other words, the higher the number of married women who were HIV+, the higher the number with candidal yeast infection; in women ≤ 39 years old (r =0.399, p < 0.05), civil servants (r = 0.328, p < 0.05), self/unemployed (r = 0.281, p < 0.05), and among university and secondary school students (r = 0.263, p < 0.05). All diabetic women (100%, n=106) had yeast infection, although only 3.8% of them were HIV-infected. Intervention programmes against yeast infections in HIV-infected women should be targeted at the vulnerable groups of women.[...] Read More.
Keywords: HIV, candida infection, HIV/Candida co-infection, pregnancy, diabetes, Idoma women.
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Full Length Research Paper
Abstract: Crude extracts of sixteen seeds belonging to different plant species were tested for their antiviral activity against herpes simplex virus-1 (HSV-1) and hepatitis A virus-27 (HAV-27). Non-toxic concentration (20 µg/ml) of Dianthus caryophyllus and Lupinus termes seed extracts to both Vero and HepG2 cells showed potent antiviral activity against HSV-1 and HAV-27 using plaque infectivity count assay. The mechanism of action D. caryophyllus revealed its virucidal activity against HSV-1 and HAV-27 as 92.3 and 92.6%, respectively, while, the virucidal activity of L. termes was observed only against HAV-27 giving 93.7% of inhibition. No effect was detected for both extracts on adsorption or on the stages of virus replication. A comparison has been done between the antiviral activity of two therapeutic drugs (Acyclovir and Amentadine used as controls for HSV-1 and HAV-MBB, respectively) and the two tested seed extracts. The results revealed that these seed extracts were more efficient in their inhibitory activity than synthetic chemical drugs against the same viruses. This may open the way to give more attention to use the natural botanical origin in treatmenting viral infection with or without therapeutic agents to obtain better recovery with least side effects.[...] Read More.
Keywords: Antiviral seed extract, herpes virus infection, hepatitis virus infection, amantadine, acyclovir.
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Full Length Research Paper
Abstract: Acinetobacter baumannii has emerged as a significant nosocomial pathogen, particularly in intensive care units. Isolates of A. baumannii resistant to major groups of antibiotics have been identified. These multi-drug resistant (MDR) organisms are limiting the treatment options. The study was performed to determine the in vitro activity of aminoglycosides, lactam- lactamase inhibitor combinations and tetracyclines against MDR A. baumannii, isolated from a tertiary care hospital. The study was carried out from January, 2009 - August, 2009, at the Department of Microbiology, Army Medical College/National University of Sciences and Technology, Rawalpindi, Pakistan looking after an 1100 bedded tertiary care hospital. Routine clinical specimens were received from various wards. A. baumannii was identified by using standard microbiological procedures. Antimicrobial susceptibility test (gentamicin, amikacin, tobramycin, ampicillin-sulbactam, piperacillin-tazobactam, cefoperazone-sulbactam, tetracycline, doxycycline, minocycline, tigecycline,) was performed according to CLSI guidelines using Kirby- Bauer disc diffusion technique. Resistance to carbapenems, fluoroquinolones and the beta-lactams were observed in significant proportion of fifty isolates. Among the aminoglycosides, the isolates were more susceptible to tobramycin than gentamicin and amikacin. Cefoperazone-sulbactam was superior to piperacillin-tazobactam and ampicillin -sulbactam in activity against MDR A. baumannii. Both tigecycline and minocycline were the active agents against most isolates. Multidrug resistant Acinetobacter infections are posing an increasing threat to our population. Minocycline, tobramycin and cefoperazone-sulbactam provide an effective option against infections caused by resistant A. baumannii.[...] Read More.
Keywords: Aminoglycosides, -lactam/ -lactamase inhibitor combinations, multi-drug resistant Acinetobacter, tetracyclines.
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