Advanced Journal of Microbiology Research

Abstract: Understanding the structure and dynamics of a docosa hexaenoic acid (DHA) containing phospholipid monomer within membranes is essential for recognizing the bilayer function in central nervous system. It has been recognized that the electrical impulses in nerve cells arise from the movement of electrical charges in the form of ions across the plasma membrane. In this study, we have modeled a novel DHA-containing phosphatidylcholine (PC) found in a marine single cell eukaryote, “Schizochytrium sp F26-b” and we were f…

Abstract: Plumbago species (Family-Plumbaginaceae) are one of the most important medicinal plants which are used for anti- inflammatory, antimicrobial diseases. Our work involves the study of anti inflammatory and cytotoxic effects of Plumbago zeylanica. The root of P. zeylanica extracted with methanol was used for determining the anti inflammatory effects. The methanolic extracts at 300 and 500 mg/kg produced 31.03 and 60.3% inhibition of acute inflammation, respectively, in Carrageenin induced raw paw oedema confirming tha…

Abstract: This study evaluated the effect of frequently used veterinary wound antimicrobials for their efficacy in killing mature in vitro Staphylococcus aureus biofilms and inhibiting planktonic cells. The predictiveness of the minimum biofilm eradication concentration (MBEC) assay as a tool for antibiotic susceptibility testing was also assessed. The minimum inhibitory concentration (MIC) and MBEC of tetracycline, tetracycline-based commercial wound spray, silver nitrate, gentian violet, iodine tincture, sucrose and a labo…

Abstract: Several mechanisms can inhibit mycelium formation which is an essential step for Candida albicans virulence. In this study, two essential oils of Melaleuca alternifolia and Eucalyptus globulus were tested for their ability to inhibit the mycelium formed on Lee medium by oral C. albicans. Lee medium was added with different concentrations of M. alternifolia essential oil ranging from 0.156 to 10 mg/ml and from 0.039 to 10 mg/ml for E. globulus. The results obtained showed that 5/17 of C. albicans strains were strong…

Abstract: Combined effects of milk non-fat dry matter content (4, 8 or 12%), incubation temperature (37, 40 or 44°C) and final pH of fermentation (4.2 or 4.5) on biochemical and microbiological characteristics of probiotic fermented milk (Lactobacillus acidophilus LA-5, Bifidobacterium lactis BB-12, and yogurt bacteria) during and immediately after fermentation were investigated. Treatments containing higher dry matter contents had slower pH and redox potential decline rates, faster acidity increase rate, longer incubation t…

Abstract: Bovine immunodeficiency virus (BIV), a member of the family Retroviridae, is an infectious pathogenic lentivirus in cattle. Although, BIV induced cattle infections are reported in several countries of the world, its prevalence in Iran is not clearly known. In this investigation, we report the detection of proviral DNA sequence of BIV in 300 blood samples of cattle by polymerase chain reaction (PCR) using oligonucleotiode primers specific for the gag gene region of the virus. Blood samples were taken from Chaharmaha…

Abstract: Polycystic ovarian syndrome is a term used to describe women who have a tendency, because of abnormal ovulation to develop multiple small cysts on their ovaries. In this study, we investigated the abnormal levels of serum inhibin B, follistatin and activin A in patients with polycystic ovary syndrome (PCOS). Serum levels of inhibin B, follistatin and activin A on cycle day 3 were measured both in PCOS group and in controls (women with regular cycle). The difference between the two groups and relationship of inhibin…

Abstract: The ethanol extracts obtained from Ballota acetabulosa (L.) Benth (Lamiaceae) were investigated for their antimicrobial activity against Bacillus cereus ATCC 7064, Bacillus subtilis ATCC 6633, Stapylococcus aureus ATCC 6538P, Escherichia coli ATCC 10538, Proteus vulgaris ATCC 6899, Salmonella typhimurium CCM 5445, Psuedomonas aeruginosa ATCC 27853, Debaryomyces hansenii DSM 70238, Kluyveromyces fragilis ATCC 8608 and Rhodotorula rubra DSM 70403 by disc diffusion method and micro dilution method. The extracts showed…

Abstract: Cucurbits ( Cucurbitaceae) are among the important plant families providing edible products to humans worldwide. It is known fact that various pathogenic fungi can attack the plants and cause diseases and resultantly yield losses. The aim of this study was to isolate and identify the plant pathogenic fungi from cucurbit plants and to evaluate their pathogenicity on cucurbits. A total of 101 cucurbit samples infected with diseases were collected from different places in Kermanshah Province, Iran. From these samples…

Abstract: Aeromonas producing extended-spectrum ß- lactamases (ESBLs) have been reported in many countries, but there is no information on the prevalence of ESBL-producing clinical Aeromonas in South African. A total of 230 isolates of Aeromonas hydrophila were isolated from 660 stool samples and 709 water samples collected in different municipalities in Limpopo province, South Africa over a period of three month. Isolates were screened for the production of ESBLs by the double disk diffusion test and for AmpC production by…

Abstract: Organic farming is increasing in proportion every year with almost all cash crops being produced by it. Pulses also can be produced using organic farming. Four different samples of moong dhal grown by conventional farming and organic farming were collected and analyzed qualitatively using FTIR (fourier transform infrared) spectroscopy. Samples were conventional moong dhal, cheaper and easily available in market, organic white, organic green leaf and organic green complete moong dhal have been taken. Peak difference…

Abstract: Fifty five thermophilic Campylobacter spp. strains were isolated from cecum of broilers, cecum and colon of pigs and from human feces. The strains were identified as Campylobacter jejuni and Campylobacter coli. The more prevalent species in broilers and humans was C. jejuni and in pigs C. coli. In the framework of this study, sensitivity to tetracycline in isolated strains of C. jejuni and C. coli was tested by E-test. In 16 tested strains isolated from broilers, 56.25% were resistant to tetracycline. Resistance oc…

Abstract: In order to find native strains of Bacillus thuringiensis, toxic to some agricultural pests, a survey was conducted in West Azerbaijan province. B. thuringiensis strains were isolated using acetate selection method with different concentrations. The morphology of crystals was studied using light microscopy. Bioassay tests were conducted on Culex pipiens (L.) as well as Pieris brassica (L.). Biochemical tests performed to identify the isolated strains. Based on the results, 48 B. thuringiensis strains were isolated…

Abstract: An Antarctic bacterial isolate was found to exhibit the extracellular xylanolytic activity. Based on 16S rRNA gene sequence analysis, the strain was named Paenibacillus sp. KIJ1. The maximum xylanase production was achieved by growing Paenibacillus sp. KIJ1 in media with 0.5% carboxymethylcellulose and 0.5% yeast extract, which were found to be the best sources of carbon and nitrogen, respectively. Optimal enzyme activity occurred at 50°C and pH 6.0. The xylanase was very specific for xylan with little or no activi…

Abstract: The purpose of the study was to evaluate the antagonistic activity of probiotics isolated from products to germination of Candida albicans in vitro. The spent culture supernatant, live bacteria, heat-killed bacteria of 16 strains of probiotics and main bacterial short-chain fatty acids were applied to inhibit the germination of C. albicans in vitro by crystal violet-based germ tube assay. Neutral SCS of all the probiotcis evaluated in this research could decrease germination significantly and live bacteria of Lacto…