Advanced Journal of Microbiology Research

Abstract: A total of 150 ready to use food thickeners were randomly collected from various markets in both urban and rural settings. Four of the samples tested Achi (Brachystegia eurycoma), Akpalata ( Afzelia africana), Ofor (Detarium microcarpum), and Ukpo (Mucuna flagellipes) were contaminated with fungal flora. The most common and prevalent fungi observed on incubated powdered form of food thickeners on media, were the Aspergillus group namely Aspergillus flavus, Aspergillus parasiticus and Aspergillus niger. Pure isolate…

Abstract: A bacteriophage infecting Vibrio harveyi was isolated from shrimp pond water, it was designated PW2. It infected all strains of V. harveyi but not other bacteria used in this study. The phage adsorption rate increased rapidly in the first 15 min of infection to 80% and continued to increase to 90% within 30 min of infection. The stability of phage PW2 was dependent on temperature and pH. It was inactivated by heating at 90°C for 30 min and by treating at pH 2, 3, 11and 12. From its one step growth curve, latent and…

Abstract: A rapid high-throughput, specific and sensitive (polymerase chain reaction) PCR-based assay coupled with DNA hybridization technique for the detection of deep bark canker (DBC) pathogen Brenneria rubrifaciens directly from mixed bacterial cells and in planta was developed. These result shows that the specific genetic markers have a powerful potential to detect B. rubrifaciens directly from crude samples at field conditions.[...]

Abstract: Skin and digestive fat content (DFC) oils from Rhynchophorus palmarum L. larva (Curculionidae) were extracted and their physicochemical properties were characterized. Water content (0.41 %) of skin oil was higher than the amount of DFC (0.04 %). While, the lipid fraction of the skin (35.16%) was slightly lower than the DFC (49.05%). The fatty acid compositions of the both oils were determined. Results showed that the most abundant fatty acids in skin and DFC oils were palmitic and oleic acids. In both oils, oleic f…

Abstract: A lignolytic basidiomycete fungus, Funalia trogii (Berk.), was isolated from decayed wood in coastal Tanzania and cultivated in submerged culture. Initially screened crude enzyme filtrate showed complete rhemazol brilliant blue - R (RBBR) decolorization 2,2’-azino-bis (3-ethylbenzthiazoline)-6-sulfonate and guaiacol oxidation after 7 days of incubation. The fungal filtrate had maximum laccase activity of 593 U/ml after 15 days of incubation. A laccase was purified by anion exchange and size exclusion chromatography…

Abstract: This study investigated the antioxidant capacity and the levels of enhanced total carotenoids (TCAR), tocopherols (TOC) and phenolic (TPC) and protein (PC) contents in whole grains of wheat plants irrigated 10 and 20% (v/v) seawater (SW) in response to water extracts of microalgae Spirulina maxima (SME) and Chlorella ellipsoida (CEE) and exogenous plant growth enhancers of ascorbic acid (Vit. C) and benzyladinin (BA) treatments. Significant differences (P < 0.05) in amounts of TCAR (ranged 80 to 140 µg/g), TOC (ran…

Abstract: A major feature of infection with trypanosomes is the development of anaemia. In this study, the effect of Scoparia dulcis ( Atiotiousha in Akoko-Edo) on Trypanosoma brucei induced anaemia was investigated in fifteen rabbits divided into three groups of n = 5 over a period of twenty eight days. Changes in Packed cell volume (PCV), Haemoglobin (Hb) concentration, Red blood cell count (RBC), Mean cell haemoglobin (MCH), Mean cell haemoglobin concentration, (MCHC) and Mean cell volume (MCV) were monitored over the per…

Abstract: Herbal drug therapy is a common practice adopted in traditional and alternative medicine and has been used in the treatment of neonatal jaundice or hyperbilirubinemia from ancient times. This is done with the help of different herbal extracts and using different treatment methods. The knowledge of new possibilities and the fact that herbal drugs have been used in some communities for a long time, clarifies the importance of studying herbal drugs. Herbal drug therapies are used commonly which amplifies the need to e…

Abstract: Cardiovascular disease (CVD) is a leading cause of mortality globally. Reducing dietary fat and cholesterol intake is central to the reduction in the risk of CVD. In Nigeria and other developing countries, meat is a good source of protein and other nutrients, and its consumption is increasing. This study investigates the cholesterol concentration of ten parts of bovine meat – rib muscle, fore-limb muscle, hind-limb muscle, lungs, large intestine, small intestine, colon, liver, kidney and heart. Standard biochemical…

Abstract: Industrialization has excessively modified the discharge and distribution of arsenic in the environment through natural and anthropogenic activities. Gastrointestinal tract, Lung and Dermal absorptions account for various adverse effects associated with arsenic toxicity. The knowledge of arsenic biotransformation holds the trivalent species (DMA3+ and MMA3+) accountable for most arsenic toxicity with mechanisms of action such as the inhibition of DNA replicating or repair enzymes, interference with tissue respirati…

Abstract: Study was undertaken to determine the efficacy of defatted soyflour mix levels in Gulabjamun (sweet dessert comprised of fried milk balls dipped in sugar syrup) and its impact on the quality parameters. Soy flour was fortified in three levels (3.33, 6.66 and 9.99%) w/w to prepare different compositions of Gulabjamuns by replacing wheat-flour in control recipe. 10 g spherical shape balls were made using thoroughly mixed ingredients dough and these were deep -fried in oil before soaking in sugar syrup (50°Brix for 4…

Abstract: Sickle cell disease (SCD) results from a mutation in the hemoglobin inside the red blood cells, where a glutamic acid at position 6 is replaced by a valine. Many phytomedicines have been identified as potential antisickling agents, stemming from reported usage as ethnomedicines by the local folk. This research examined methanolic leaf extracts of Carica papaya L. (Caricaceae) for possible in vitro antisickling and membrane-stabilizing activities involving the use of positive (p-hydroxybenzoic acid 5 mg/ml) and nega…

Abstract: In the present study, the hepatoprotective activity of methanolic extract of Apium graveolens L. (celery) seeds was tested against Di-(2- ethylhexyl) phthalate (DEHP) induced hepatotoxicity in rats. Oral administration of DEHP (1000 mg/kg b.wt/day) for 6 weeks in rats caused a significant increase in the levels of serum marker enzymes like serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), alkaline phosphatases (ALP) and the levels of total bilirubin and hepatic lipid pe…

Abstract: Herbal drug therapy is a common practice adopted in traditional and alternative medicine and has been used in the treatment of neonatal jaundice or hyperbilirubinemia from ancient times. This is done with the help of different herbal extracts and using different treatment methods. The knowledge of new possibilities and the fact that herbal drugs have been used in some communities for a long time, clarifies the importance of studying herbal drugs. Herbal drug therapies are used commonly which amplifies the need to e…

Abstract: Paraoxonase/arylesterases (EC.3.1.8.2) is an enzyme found tightly associated with high density lipoprotein particle in serum. Because of its unique enzyme activity, antioxidant property and its role as an anti atherosclerotic molecule, various methods are used for its purification from human serum. Methods involved in its purification are elaborate and complicated. Also the yield and final activity are highly variable. Here, we report a 2 step method of purification involving affinity chromatography on cibacron blu…