Advanced Journal of Microbiology Research

Abstract: In this study, effects of chlorine dioxide (0.3 and 0.5%), lactic acid (0.5 and 1.0%) and fumaric acid (0.5 and 1.0%) on the shelf-life of broiler wings were investigated. The samples were dipped into the experimental solutions for 10 min. and stored at +4°C for 9 days. Microbiologically, the counts of total aerobic mesophilic bacteria (TAMB), total psychrophilic bacteria (TPB) and ESCHERICHIA COLI were determined and pH values were measured. Compared to the control samples, the TAMB counts were reduced approximate…

Abstract: Eddy pumping is a process by which mesoscale eddies induce isopycnal displacements that lift nutrient-replete waters into the euphotic zone, driving a set of biogeochemical processes in the ocean. To investigate the potential effect of physical processes on the microbial community functions, the microbial community metaproteomic profiles were determined in a cold-core cyclonic eddy in the South China Sea (SCS). The significant differences of metaproteomic patterns accurately predicted their distinct functional resp…

Abstract: An extra-cellular thermo-alkaliphillic protease producing Bacillus was isolated from the soil and identified to be Bacillus tequilensis MTCC 9585 by microscopic, colony morphology, biochemical and 16S ribotyping. B. tequilensis MTCC 9585 produces protease up to 21 h of growth but interestingly 90% of the protease production occurred, just after 6 h of growth. The organism grew as well as and produced enzyme at wide pH (5 to 12) and temperature range (4, 25, 37 and 50°C), though optimum temperature and pH for the gr…

Abstract: Brucellosis is an important disease affecting mainly sheep and goats. Diagnosis based on isolation of Brucella organisms from the suspected animals is the golden standard but has a limited sensitivity, expensive and unpractical to apply on a large scale in control campaigns. Accordingly, the indirect diagnosis of disease based on serological tests is the method of choice in the eradication programs. In this study, a single step polymerase chain reaction (PCR) was used to diagnose brucellosis using sheep whole blood…

Abstract: In vitro antifungal activity of methanol, acetone and ethyl-acetate extracts from Equisetum telmateia Ehrh., Allium flavum L., Sedum acre L., Sideritis montana L., Marubium peregrinum L. and Xeranthemum anuum L. grown in Serbia, were investigated by microdilution method. Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) have been determined. Testing was conducted against four Aspergillus species - A. flavus, A. fumigatus, A. niger and A. restrictus. All statistical analyses were perf…

Abstract: Indirect Haemaglutination test is a reliable quantitative serological test for measuring antibodies against angara disease. The etiological agent contained in the liver of affected chick did not directly haemaglutinate erythrocyte of chickens. The antibody titre measured through IHA varied from 1:8-1: 128, in the sera (samples) of experimentally infected chickens at days 7, 14 and 21 post vaccination.[...]

Abstract: A total of 34 isolates of bacteria, 20 Staphylococus aureus and 14 Clostridium perfringens from leachate samples, were investigated for virulence factors and antibiotic resistance. Majority (>70%) of the isolates produced enterotoxins. Many of the S. aureus isolates tested positive for deoxyribonuclease, haemolysins and slime production. Staphylococcal enterotoxin A (SEA) was the predominant enterotoxin produced by the S. aureus. None of the S. aureus was resistant to ciprofloxacin, novobiocin and vancomycin. Six i…

Abstract: This study aims at investigating the status of bacterial contamination of four daily used objects, computer keyboards, computer mice, elevator buttons and shopping carts handles. A total of 400 samples were collected from 4 different objects; 100 from each. Samples were collected from different places (offices, internet cafes, homes, buildings and supermarkets) in the city of Jeddah, Saudi Arabia. 95.5% of the total samples collected were contaminated with mixed bacterial growth. Coagulase-negative staphylococci do…

Abstract: The gene encoding a thermophilic group 3 alcohol dehydrogenase from Thermotoga lettingae TMO was cloned and over-expressed in Escherichia coli. The full-length DNA sequence of TlADH was 1086-bp encoding a polypeptide of 361 amino acids. Comparative and bioinformatic analysis revealed that TlADH showed high similarity to group 3 ADHs from thermophilic and mesophilic alcohol dehydrogenases, but relatively highest similarity to T. maritime 1, 3-propanediol dehydrogenase. The optimal pH-values of butanol oxidation and…

Abstract: The influence of bacterial suspension concentration on biocontrol efficacies and storage stabilities of three biocontrol agents, including BACILLUS CEREUS AR156, PANTOEA ANANATIS YT11 and PSEUDOMONAS FLUORESCENS ABc9, were studied. The results indicated that biocontrol efficacy and storage stability did not increase along with the enhancement of the concentration of biocontrol agents, and 109 CFU ml-1 of the strain P. FLUORESCENS ABc9, 108 CFU ml-1 of B. CEREUS AR156 and 108 CFU ml-1 of P. ANANATIS YT11 provided th…

Abstract: Due to the production of abundant capsular polysaccharides and or exopolysaccharides, some bacteria form mucoid and opaque colonies on solid agar medium. Polysaccharides interfere with the isolation and characterization of DNA. Extraction of polysaccharide-free DNA from the mucoid bacteria is required for genetic and biochemical studies. In this study, mucoid Vibrio parahaemolyticus and Klebsiella pneumonia were used as model organisms for comparing the effectiveness of two DNA extraction methods. The two methods w…

Abstract: Microsatellite as one of the most popular molecular markers provides a useful tool for population genetic structure analysis in insects. But few microsatellite loci have been developed for Lepidoptera. Here we isolated and characterized 9 polymorphic microsatellite loci in Rhyacionia leptotubula (Lepidoptera: Tortricidae). The number of alleles of these loci ranged from 2 to 11. Observed and expected heterozygosities ranged from 0.0000 to 0.5294 and from 0.3905 to 0.8719, respectively. Seven loci show significant H…

Abstract: Lactic acid bacteria (LAB) are one of the most applied bacteria in the production of fermented foods, from dairy to fruits and vegetables products. They make food durable, improve food safety, flavor, and texture as well as to enhance food physiological and hygienic value due to the presence of viable cells and valuable. Besides, products obtained by LAB fermentation processes are of special importance for functional foods such as probiotics. Lactobacillus acidophilus is the best well-known species of this Lactobac…

Abstract: The methanol and dichloromethane extracts of Inula cappa (Ham. ex D. Don) DC. were evaluated for inhibition of herpes simplex virus infection. Higher anti- herpes simplex virus (HSV) activity of I. cappa methanol extract was observed comparing with I. cappa dichloromethane extract since more than 50% of the virus was inhibited by I. cappa methanol extract and 50% effective doses of I. cappa methanol extract on HSV-1and HSV-2 were 720.1±32.7 and 529.2±5.2 µg/ml respectively. However, when the virus particles were tr…

Abstract: Penicillium sp. was isolated from Daqu as a beginner strain. In order to investigate the role of Penicillium sp. in Luzhou-flavor liquor, Penicillium sp. was operated in Koji seeds and then simulated solid-state fermentation for liquor testing under different conditions. They included adding of different amounts of koji seeds and Daqu. The results showed that, when the amount of Daqu added was 20%, Penicillium sp. koji added increased from 0.5 to 2%, compared to blank control group. Liquor yield was reduced by 2.23…